{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T17:26:12Z","timestamp":1789579572265,"version":"build-2803163510"},"reference-count":22,"publisher":"Informa UK Limited","issue":"4","license":[{"start":{"date-parts":[[2020,12,22]],"date-time":"2020-12-22T00:00:00Z","timestamp":1608595200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP19ae0101045"],"award-info":[{"award-number":["JP19ae0101045"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,3,24]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>A novel methymycin analog, 12-ketomethymycin N-oxide, was produced by the heterologous expression of the pikromycin\/methymycin biosynthetic gene cluster of Streptomyces sp. AM4900 together with 12-ketomethymycin, which was only isolated by the biotransformation of the synthetic intermediate before. Their structures were determined by the spectroscopic data and the chemical derivatization. 12-Ketomethymycin showed a weak cytotoxicity against SKOV-3 and Jurkat cells, although its N-oxide analog did not show any activity. Both showed no antibacterial activities against Escherichia coli and Micrococcus luteus.<\/jats:p>","DOI":"10.1093\/bbb\/zbaa111","type":"journal-article","created":{"date-parts":[[2021,2,8]],"date-time":"2021-02-08T10:15:54Z","timestamp":1612779354000},"page":"890-894","source":"Crossref","is-referenced-by-count":4,"title":["A novel methymycin analog, 12-ketomethymycin <i>N<\/i>-oxide, produced by the heterologous expression of the large pikromycin\/methymycin biosynthetic gene cluster of <i>Streptomyces<\/i> sp. 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