{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T20:56:38Z","timestamp":1765486598268,"version":"3.37.3"},"reference-count":29,"publisher":"Informa UK Limited","issue":"7","license":[{"start":{"date-parts":[[2021,4,30]],"date-time":"2021-04-30T00:00:00Z","timestamp":1619740800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["17K07735"],"award-info":[{"award-number":["17K07735"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,6,24]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The fungus Exophiala jeanselmei strain KUFI-6N produces a unique cycloalkanone monooxygenase (ExCAMO) that displays an uncommon substrate spectrum of Baeyer\u2013Villiger oxidation of 4-10-membered ring ketones. In this study, we aimed to identify and sequence the gene encoding ExCAMO from KUFI-6N and overexpress the gene in Escherichia coli. We found that the primary structure of ExCAMO is most closely related to the cycloalkanone monooxygenase from Cylindrocarpon radicicola ATCC 11011, with 54.2% amino acid identity. ExCAMO was functionally expressed in E. coli and its substrate spectrum and kinetic parameters were investigated. Substrate profiling indicated that ExCAMO is unusual among known Baeyer\u2013Villiger monooxygenases owing to its ability to accept a variety of substrates, including C4-C12 membered ring ketones. ExCAMO has high affinity and catalytic efficiency toward cycloalkanones, the highest being toward cyclohexanone. Five other genes encoding Baeyer\u2013Villiger monooxygenases were also cloned and expressed in E. coli.<\/jats:p>","DOI":"10.1093\/bbb\/zbab079","type":"journal-article","created":{"date-parts":[[2021,4,30]],"date-time":"2021-04-30T17:19:32Z","timestamp":1619803172000},"page":"1675-1685","source":"Crossref","is-referenced-by-count":4,"title":["Cloning, expression, and characterization of Baeyer\u2013Villiger monooxygenases from eukaryotic <i>Exophiala jeanselmei<\/i> strain KUFI-6N"],"prefix":"10.1080","volume":"85","author":[{"given":"Taisei","family":"Yamamoto","sequence":"first","affiliation":[{"name":"Department of Life Science and Biotechnology, Kansai University, Suita, Osaka, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kento","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Life Science and Biotechnology, Kansai University, Suita, Osaka, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshie","family":"Hasegawa","sequence":"additional","affiliation":[{"name":"Department of Life Science and Biotechnology, Kansai University, Suita, Osaka, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroaki","family":"Iwaki","sequence":"additional","affiliation":[{"name":"Department of Life Science and Biotechnology, Kansai University, Suita, Osaka, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"301","published-online":{"date-parts":[[2021,4,30]]},"reference":[{"key":"2021070811181080700_bib1","doi-asserted-by":"crossref","first-page":"3625","DOI":"10.1002\/cber.189903203151","article-title":"Einwirkung des Caro\u2019schen Reagens auf Ketone","volume":"32","author":"Baeyer","year":"1899","journal-title":"Ber Dtsch Chem Ges"},{"key":"2021070811181080700_bib2","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1093\/nar\/7.6.1513","article-title":"A rapid 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