{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T20:14:06Z","timestamp":1790108046462,"version":"4.0.1"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T00:00:00Z","timestamp":1662508800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82104722"],"award-info":[{"award-number":["82104722"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Suzhou Youth Science and Education Project","award":["KJXW2018075"],"award-info":[{"award-number":["KJXW2018075"]}]},{"name":"Suzhou Science and Technology Department","award":["SKJY2021129"],"award-info":[{"award-number":["SKJY2021129"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,10,20]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Garlic is a popular culinary herb for the prevention and treatment of alcoholic liver disease (ALD). Diallyl Trisulfide (DATS) is the major organosulfur compound of garlic. Latest studies indicated that the hepatocyte pyroptosis serves a primary role in the pathogenesis of ALD. The present study aims to assess the inhibitory effect of DATS on alcohol-induced hepatocyte pyroptosis, and to elucidate the potential mechanism by using the hepatocyte cell line HL-7702. Our study found that DATS inhibited alcohol-induced pyroptosis by decreasing gasdermin D (GSDMD) activation. Results illuminated that DATS inhibited alcohol-induced (NOD)-like receptor containing pyrin domain 3 (NLRP3) inflammasome activation by reducing intracellular reactive oxygen species (ROS) accumulation. Furthermore, DATS upregulated hydrogen sulfide (H2S) to resist ROS overproduction. The present study demonstrated that DATS mitigated alcohol-induced hepatocyte pyroptosis by increasing the intracellular level of H2S.<\/jats:p>","DOI":"10.1093\/bbb\/zbac149","type":"journal-article","created":{"date-parts":[[2022,9,8]],"date-time":"2022-09-08T09:01:15Z","timestamp":1662627675000},"page":"1552-1561","source":"Crossref","is-referenced-by-count":12,"title":["Diallyl Trisulfide attenuates alcohol-induced hepatocyte pyroptosis via elevation of hydrogen sulfide"],"prefix":"10.1093","volume":"86","author":[{"given":"Xiaojing","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine , Suzhou, Jiangsu , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongxin","family":"Lu","sequence":"additional","affiliation":[{"name":"Clinical Pharmaceutical Laboratory of Traditional Chinese Medicine, Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine , Suzhou, Jiangsu , China"},{"name":"Clinical Pharmaceutical Laboratory of Traditional Chinese Medicine, Suzhou Academy of Wumen Chinese Medicine , Suzhou, Jiangsu , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Genrong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine , Suzhou, Jiangsu , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine , Suzhou, Jiangsu , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongjiu","family":"Zhan","sequence":"additional","affiliation":[{"name":"Department of Dermatologists, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine , Suzhou, Jiangsu , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoxin","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine , Suzhou, Jiangsu , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0188-2044","authenticated-orcid":false,"given":"Liang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Clinical Pharmaceutical Laboratory of Traditional Chinese Medicine, Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine , Suzhou, Jiangsu , China"},{"name":"Clinical Pharmaceutical Laboratory of Traditional Chinese Medicine, Suzhou Academy of Wumen Chinese Medicine , Suzhou, Jiangsu , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,9,7]]},"reference":[{"key":"2022102012373501500_bib1","doi-asserted-by":"crossref","first-page":"955S","DOI":"10.1093\/jn\/131.3.955S","article-title":"Intake of garlic and its bioactive components","volume":"131","author":"Amagase","year":"2001","journal-title":"J Nutr"},{"key":"2022102012373501500_bib2","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1002\/biof.5520260201","article-title":"Antithrombotic and anticancer effects of garlic-derived sulfur compounds: a review","volume":"26","author":"Ariga","year":"2006","journal-title":"Biofactors"},{"key":"2022102012373501500_bib3","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1016\/j.bbamcr.2018.02.010","article-title":"Nrf2-Keap1 signaling in oxidative and reductive stress","volume":"1865","author":"Bellezza","year":"2018","journal-title":"Biochim Biophys Acta Mol Cell Res"},{"key":"2022102012373501500_bib4","first-page":"127","article-title":"Spectrophotometric analysis of human CYP2E1-catalyzed p-nitrophenol hydroxylation","volume":"320","author":"Chang","year":"2006","journal-title":"Methods Mol Biol"},{"key":"2022102012373501500_bib5","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.biopha.2016.01.009","article-title":"Diallyl trisulfide attenuates ethanol-induced hepatic steatosis by inhibiting oxidative stress and apoptosis","volume":"79","author":"Chen","year":"2016","journal-title":"Biomed Pharmacother"},{"key":"2022102012373501500_bib6","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.intimp.2016.04.015","article-title":"Diallyl trisulfide protects against ethanol-induced oxidative stress and apoptosis via a hydrogen sulfide-mediated mechanism","volume":"36","author":"Chen","year":"2016","journal-title":"Int Immunopharmacol"},{"key":"2022102012373501500_bib7","doi-asserted-by":"crossref","first-page":"114140","DOI":"10.1016\/j.jep.2021.114140","article-title":"Protection of Ficus pandurata Hance against acute alcohol-induced liver damage in mice via suppressing oxidative stress, inflammation, and apoptosis","volume":"275","author":"Dai","year":"2021","journal-title":"J Ethnopharmacol"},{"key":"2022102012373501500_bib8","doi-asserted-by":"crossref","first-page":"110610","DOI":"10.1016\/j.ecoenv.2020.110610","article-title":"Caffeic acid phenethyl ester against cadmium induced toxicity mediated by CircRNA modulates autophagy in HepG2 cells","volume":"197","author":"Hao","year":"2020","journal-title":"Ecotoxicol Environ Saf"},{"key":"2022102012373501500_bib9","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.1016\/j.tibs.2016.09.002","article-title":"Mechanism and regulation of NLRP3 inflammasome activation","volume":"41","author":"He","year":"2016","journal-title":"Trends Biochem Sci"},{"key":"2022102012373501500_bib10","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1136\/gutjnl-2017-315123","article-title":"Alcohol dysregulates miR-148a in hepatocytes through FoxO1, facilitating pyroptosis via TXNIP overexpression","volume":"68","author":"Heo","year":"2019","journal-title":"Gut"},{"key":"2022102012373501500_bib11","doi-asserted-by":"crossref","first-page":"7606","DOI":"10.1073\/pnas.1321232111","article-title":"Reactive cysteine persulfides and S-polythiolation regulate oxidative stress and redox signaling","volume":"111","author":"Ida","year":"2014","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2022102012373501500_bib12","doi-asserted-by":"crossref","DOI":"10.3390\/ijms21239100","article-title":"Garlic derived diallyl trisulfide in experimental metabolic syndrome: metabolic effects and cardioprotective role","volume":"21","author":"Jeremic","year":"2020","journal-title":"Int J Mol Sci"},{"key":"2022102012373501500_bib13","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.freeradbiomed.2020.03.031","article-title":"Oroxylin a promotes PGC-1alpha\/Mfn2 signaling to attenuate hepatocyte pyroptosis via blocking mitochondrial ROS in alcoholic liver disease","volume":"153","author":"Kai","year":"2020","journal-title":"Free Radical Biol Med"},{"key":"2022102012373501500_bib14","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1038\/nature15541","article-title":"Caspase-11 cleaves gasdermin D for non-canonical inflammasome signalling","volume":"526","author":"Kayagaki","year":"2015","journal-title":"Nature"},{"key":"2022102012373501500_bib15","doi-asserted-by":"crossref","first-page":"1737","DOI":"10.1002\/hep.29645","article-title":"Pyroptosis by caspase11\/4-gasdermin-D pathway in alcoholic hepatitis in mice and patients","volume":"67","author":"Khanova","year":"2018","journal-title":"Hepatology"},{"key":"2022102012373501500_bib16","doi-asserted-by":"crossref","first-page":"697","DOI":"10.3350\/cmh.2020.0152","article-title":"Oxidative stress and glutamate excretion in alcoholic steatosis: metabolic synapse between hepatocyte and stellate cell","volume":"26","author":"Kim","year":"2020","journal-title":"Clin Mol Hepatol"},{"key":"2022102012373501500_bib17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1089\/ars.2008.2282","article-title":"Hydrogen sulfide increases glutathione production and suppresses oxidative stress in mitochondria","volume":"12","author":"Kimura","year":"2010","journal-title":"Antioxid Redox Signal"},{"key":"2022102012373501500_bib18","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1038\/nri3452","article-title":"Activation and regulation of the inflammasomes","volume":"13","author":"Latz","year":"2013","journal-title":"Nat Rev Immunol"},{"key":"2022102012373501500_bib19","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.intimp.2019.03.024","article-title":"Diallyl Trisulfide can induce fibroblast-like synovial apoptosis and has a therapeutic effect on collagen-induced arthritis in mice via blocking NF-\u03baB and Wnt pathways","volume":"71","author":"Liang","year":"2019","journal-title":"Int Immunopharmacol"},{"key":"2022102012373501500_bib20","doi-asserted-by":"crossref","DOI":"10.3390\/nu10111665","article-title":"Ginseng (Panax ginseng Meyer) oligopeptides protect against binge drinking-induced liver damage through inhibiting oxidative stress and inflammation in rats","volume":"10","author":"Liu","year":"2018","journal-title":"Nutrients"},{"key":"2022102012373501500_bib21","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1111\/jgh.12389","article-title":"Effects of treatment with hydrogen sulfide on methionine-choline deficient diet-induced non-alcoholic steatohepatitis in rats","volume":"29","author":"Luo","year":"2014","journal-title":"J Gastroenterol Hepatol"},{"key":"2022102012373501500_bib22","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.niox.2014.02.006","article-title":"Hydrogen sulfide and the liver","volume":"41","author":"Mani","year":"2014","journal-title":"Nitric Oxide"},{"key":"2022102012373501500_bib23","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/bs.acc.2015.12.007","article-title":"Medical functions of hydrogen sulfide","volume":"74","author":"Olas","year":"2016","journal-title":"Adv Clin Chem"},{"key":"2022102012373501500_bib24","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1007\/s11906-019-1009-9","article-title":"Effect of garlic's active constituents in inflammation, obesity and cardiovascular disease","volume":"22","author":"Quesada","year":"2020","journal-title":"Curr Hypertens Rep"},{"key":"2022102012373501500_bib25","doi-asserted-by":"crossref","first-page":"11104","DOI":"10.1021\/jf303800p","article-title":"Transcriptome analysis of garlic-induced hepatoprotection against alcoholic fatty liver","volume":"60","author":"Raghu","year":"2012","journal-title":"J Agric Food Chem"},{"key":"2022102012373501500_bib26","doi-asserted-by":"crossref","first-page":"486","DOI":"10.2174\/1389200216666150812123554","article-title":"Diallyl sulfide: potential use in novel therapeutic interventions in alcohol, drugs, and disease mediated cellular toxicity by targeting cytochrome P450 2E1","volume":"16","author":"Rao","year":"2015","journal-title":"Curr Drug Metab"},{"key":"2022102012373501500_bib27","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10010049","article-title":"Disrupted H2S signaling by cigarette smoking and alcohol drinking: evidence from cellular, animal, and clinical studies","volume":"10","author":"Read","year":"2021","journal-title":"Antioxidants (Basel)"},{"key":"2022102012373501500_bib28","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1017\/S0007114520001403","article-title":"Effect of garlic powder supplementation on hepatic steatosis, liver enzymes and lipid profile in patients with non-alcoholic fatty liver disease: a double-blind randomised controlled clinical trial","volume":"124","author":"Sangouni","year":"2020","journal-title":"Br J Nutr"},{"key":"2022102012373501500_bib29","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.tibs.2016.10.004","article-title":"Pyroptosis: gasdermin-mediated programmed necrotic cell death","volume":"42","author":"Shi","year":"2017","journal-title":"Trends Biochem Sci"},{"key":"2022102012373501500_bib30","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1038\/nature15514","article-title":"Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death","volume":"526","author":"Shi","year":"2015","journal-title":"Nature"},{"key":"2022102012373501500_bib31","first-page":"5084","article-title":"Chemoprevention of 1,2-dimethylhydrazine-induced colon cancer in mice by naturally occurring organosulfur compounds","volume":"50","author":"Sumiyoshi","year":"1990","journal-title":"Cancer Res"},{"key":"2022102012373501500_bib32","doi-asserted-by":"crossref","first-page":"3541","DOI":"10.1038\/s41598-017-03941-0","article-title":"A long-term and slow-releasing hydrogen sulfide donor protects against myocardial ischemia\/reperfusion injury","volume":"7","author":"Sun","year":"2017","journal-title":"Sci Rep"},{"key":"2022102012373501500_bib33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.niox.2018.09.001","article-title":"Donor heart preservation with a novel long-term and slow-releasing hydrogen sulfide system","volume":"81","author":"Sun","year":"2018","journal-title":"Nitric Oxide"},{"key":"2022102012373501500_bib34","doi-asserted-by":"crossref","first-page":"e25943","DOI":"10.1371\/journal.pone.0025943","article-title":"Hydrogen sulfide attenuates carbon tetrachloride-induced hepatotoxicity, liver cirrhosis and portal hypertension in rats","volume":"6","author":"Tan","year":"2011","journal-title":"PLoS One"},{"key":"2022102012373501500_bib35","doi-asserted-by":"crossref","DOI":"10.3390\/molecules23123305","article-title":"Overview on the effects of N-Acetylcysteine in neurodegenerative diseases","volume":"23","author":"Tardiolo","year":"2018","journal-title":"Molecules"},{"key":"2022102012373501500_bib36","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1016\/j.jhep.2011.05.015","article-title":"Pathways of liver injury in alcoholic liver disease","volume":"55","author":"Tilg","year":"2011","journal-title":"J Hepatol"},{"key":"2022102012373501500_bib37","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1038\/nature22393","article-title":"Chemotherapy drugs induce pyroptosis through caspase-3 cleavage of a gasdermin","volume":"547","author":"Wang","year":"2017","journal-title":"Nature"},{"key":"2022102012373501500_bib38","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1080\/13880209.2018.1479868","article-title":"Effects of garlic polysaccharide on alcoholic liver fibrosis and intestinal microflora in mice","volume":"56","author":"Wang","year":"2018","journal-title":"Pharm Biol"},{"key":"2022102012373501500_bib39","doi-asserted-by":"crossref","first-page":"1582","DOI":"10.3389\/fimmu.2019.01582","article-title":"The roles of liver-resident lymphocytes in liver diseases","volume":"10","author":"Wang","year":"2019","journal-title":"Front Immunol"},{"key":"2022102012373501500_bib40","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1016\/S0278-6915(00)00171-X","article-title":"Effects of organosulfur compounds from garlic oil on the antioxidation system in rat liver and red blood cells","volume":"39","author":"Wu","year":"2001","journal-title":"Food Chem Toxicol"},{"key":"2022102012373501500_bib41","doi-asserted-by":"crossref","first-page":"1906","DOI":"10.1089\/ars.2012.4645","article-title":"Hydrogen sulfide protects against cellular senescence via S-sulfhydration of Keap1 and activation of Nrf2","volume":"18","author":"Yang","year":"2013","journal-title":"Antioxid Redox Signal"},{"key":"2022102012373501500_bib42","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1007\/s00204-015-1543-4","article-title":"Experimental models of liver fibrosis","volume":"90","author":"Yanguas","year":"2016","journal-title":"Arch Toxicol"},{"key":"2022102012373501500_bib43","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1093\/toxres\/tfac005","article-title":"Hepatoprotective effect of diallyl trisulfide against lipopolysaccharide and D-galactosamine induced acute liver failure in mice via suppressing inflammation and apoptosis","volume":"11","author":"Yu","year":"2022","journal-title":"Toxicol Res (Camb)"},{"key":"2022102012373501500_bib44","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.tox.2008.07.062","article-title":"Diallyl trisulfide (DATS) effectively attenuated oxidative stress-mediated liver injury and hepatic mitochondrial dysfunction in acute ethanol-exposed mice","volume":"252","author":"Zeng","year":"2008","journal-title":"Toxicology"},{"key":"2022102012373501500_bib45","doi-asserted-by":"crossref","first-page":"1406726","DOI":"10.1155\/2017\/1406726","article-title":"Diallyl trisulfide suppresses oxidative stress-induced activation of hepatic stellate cells through production of hydrogen sulfide","volume":"2017","author":"Zhang","year":"2017","journal-title":"Oxid Med Cell Long"},{"key":"2022102012373501500_bib46","doi-asserted-by":"crossref","first-page":"2976","DOI":"10.3892\/mmr.2017.6856","article-title":"Involvement of exogenous 3deoxyglucosone in betacell dysfunction induces impaired glucose regulation","volume":"16","author":"Zhang","year":"2017","journal-title":"Mol Med Rep"},{"key":"2022102012373501500_bib47","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1530\/EJE-19-0179","article-title":"Association between dietary raw garlic intake and newly diagnosed nonalcoholic fatty liver disease: a population-based study","volume":"181","author":"Zhang","year":"2019","journal-title":"Eur J Endocrinol"},{"key":"2022102012373501500_bib48","doi-asserted-by":"crossref","first-page":"873","DOI":"10.21037\/atm-21-2158","article-title":"Butyrate ameliorates alcoholic fatty liver disease via reducing endotoxemia and inhibiting liver gasdermin D-mediated pyroptosis","volume":"9","author":"Zhang","year":"2021","journal-title":"Ann Transl Med"},{"key":"2022102012373501500_bib49","doi-asserted-by":"crossref","first-page":"643149","DOI":"10.3389\/fimmu.2021.643149","article-title":"Inflammasomes and Fibrosis","volume":"12","author":"Zhang","year":"2021","journal-title":"Front Immunol"},{"key":"2022102012373501500_bib50","doi-asserted-by":"crossref","first-page":"106968","DOI":"10.1016\/j.intimp.2020.106968","article-title":"NLRP12 negatively regulates EtOH-induced liver macrophage activation via NF-kappaB pathway and mediates hepatocyte apoptosis in alcoholic liver injury","volume":"88","author":"Zhang","year":"2020","journal-title":"Int Immunopharmacol"}],"container-title":["Bioscience, Biotechnology, and Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/bbb\/advance-article-pdf\/doi\/10.1093\/bbb\/zbac149\/46355514\/zbac149.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bbb\/article-pdf\/86\/11\/1552\/46579752\/zbac149.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bbb\/article-pdf\/86\/11\/1552\/46579752\/zbac149.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T12:30:05Z","timestamp":1727958605000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bbb\/article\/86\/11\/1552\/6693931"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,7]]},"references-count":50,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2022,9,7]]},"published-print":{"date-parts":[[2022,10,20]]}},"URL":"https:\/\/doi.org\/10.1093\/bbb\/zbac149","relation":{},"ISSN":["1347-6947"],"issn-type":[{"value":"1347-6947","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2022,11]]},"published":{"date-parts":[[2022,9,7]]}}}