{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T23:42:42Z","timestamp":1787614962119,"version":"build-2736575974"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2022,12,15]],"date-time":"2022-12-15T00:00:00Z","timestamp":1671062400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["20H00570"],"award-info":[{"award-number":["20H00570"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["21H04863"],"award-info":[{"award-number":["21H04863"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Fordays Co., Ltd."}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,24]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The protamine-derived peptide arginine-proline-arginine (RPR) can ameliorate lifestyle-related diseases such as obesity and hypercholesterolemia. Thus, we hypothesized that the hypolipidemic activity of RPR could attenuate events leading to non-alcoholic fatty liver disease. Addition of 2\u00a0m\u2009m oleic acid (OA) to the culture medium induced fatty liver conditions in HepG2 cells. The OA\u00a0+\u00a0RPR group showed significantly decreased cellular or medium triglyceride (TG) level compared with the OA group. Stearoyl-CoA desaturase-1 (SCD1) or sterol regulatory element-binding protein 1 (SREBP1) protein level was significantly lower in the OA\u00a0+\u00a0RPR group than in the OA group. In the R\u00a0+\u00a0P\u00a0+\u00a0R amino acid mixture-treated group, the TG level was not significantly different from that in the OA-treated group. The OA\u00a0+\u00a0RP- or OA\u00a0+\u00a0PR-treated groups showed significantly decreased cellular TG level compared with the OA group. Moreover, the effect of RPR disappeared when the peptide transporter 1 (PepT1) was knocked down with a siRNA. Collectively, our results demonstrated that RPR effectively ameliorated hepatic steatosis in HepG2 cells via the PepT1 pathway.<\/jats:p>","DOI":"10.1093\/bbb\/zbac197","type":"journal-article","created":{"date-parts":[[2022,12,16]],"date-time":"2022-12-16T01:36:25Z","timestamp":1671154585000},"page":"197-207","source":"Crossref","is-referenced-by-count":7,"title":["Protamine-derived peptide RPR (Arg-Pro-Arg) ameliorates oleic acid-induced lipogenesis via the PepT1 pathway in HepG2 cells"],"prefix":"10.1093","volume":"87","author":[{"given":"Maihemuti","family":"Mijiti","sequence":"first","affiliation":[{"name":"Department of Applied Life Science, Faculty of Applied Biological Sciences, Gifu University , Gifu , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryosuke","family":"Mori","sequence":"additional","affiliation":[{"name":"Department of Applied Life Science, Faculty of Applied Biological Sciences, Gifu University , Gifu , 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