{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T13:58:12Z","timestamp":1785160692624,"version":"3.55.0"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T00:00:00Z","timestamp":1708905600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,4,22]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Human lysozyme (hLYZ) has attracted considerable research attention due to its natural and efficient antibacterial abilities and widespread uses. In this study, hLYZ was modified to enhance its enzyme activity and expressed in a Pichia pastoris expression system. A combination mutant HZM(2R-K)-N88D\/V110S demonstrated the highest enzyme activity (6213\u00a0\u00b1\u00a0164 U\/mL) in shake flasks, which was 4.07-fold higher when compared with the original strain. Moreover, the recombinant P. pastoris was inducted in a 3\u00a0L bioreactor plus methanol\/sorbitol co-feeding. After 120\u00a0h induction, the antibacterial activity of hLYZ reached 2.23\u00a0\u00b1\u00a00.12\u00a0\u00d7\u00a0105 U\/mL, with the specific activity increasing to 1.89\u00a0\u00d7\u00a0105 U\/mg, which is currently the highest specific activity obtained through recombinant expression of hLYZ. Also, hLYZ supernatants showed 2-fold inhibitory effects toward Staphylococcus aureus and Micrococcus lysodeikticus when compared with HZM(2R-K). Our research generated a hLYZ mutant with high antibacterial capabilities and provided a method for screening of high-quality enzymes.<\/jats:p>","DOI":"10.1093\/bbb\/zbae023","type":"journal-article","created":{"date-parts":[[2024,2,27]],"date-time":"2024-02-27T07:26:10Z","timestamp":1709018770000},"page":"546-554","source":"Crossref","is-referenced-by-count":4,"title":["The molecular modification, expression, and the antibacterial effects studies of human lysozyme"],"prefix":"10.1093","volume":"88","author":[{"given":"Kewei","family":"Xu","sequence":"first","affiliation":[{"name":"SINOPEC Key Laboratory of Petroleum Accumulation Mechanisms , Wuxi, Jiangsu , China"},{"name":"Wuxi Research Institute of Petroleum Geology, Research Institute of Petroleum Exploration & Production, SINOPEC , Wuxi, Jiangsu , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanyuan","family":"Ma","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Industrial 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