{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:13:44Z","timestamp":1787008424389,"version":"build-2736575974"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2024,4,10]],"date-time":"2024-04-10T00:00:00Z","timestamp":1712707200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009950","name":"Ministry of Education","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009950","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,6,21]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>We confirmed that the hexane layer of Hydrangea macrophylla leaf extract (HLH) is rich in phyllodulcin (PD), an alternative sweetener, through high performance liquid chromatography (HPLC) analysis. To investigate in vivo activity of HLH and its PD, acute toxicity and growth rate of Caenorhabditis elegans were tested and there are no clinical abnormalities at 125-500\u00a0\u00b5g\/mL of HLH. HLH decreased the total lipid and triglyceride contents dose-dependently in glucose-induced obese worms. Also, HLH increased survival rates under oxidative and thermal stress and decreased body reactive oxygen species (ROS) contents significantly. Such antioxidant properties of HLH were attributed to the enhanced activity of the antioxidant enzyme catalase. To determine whether the effect of HLH was due to PD, worms were treated with PD (concentration contained in HLH), and inhibitory effects on total lipids and ROS were observed. Our results suggest that HLH and its PD as a natural alternative sweetener can be used as materials to improve metabolic diseases.<\/jats:p>","DOI":"10.1093\/bbb\/zbae043","type":"journal-article","created":{"date-parts":[[2024,4,10]],"date-time":"2024-04-10T20:26:35Z","timestamp":1712780795000},"page":"789-797","source":"Crossref","is-referenced-by-count":10,"title":["Phyllodulcin from the hexane fraction of\n                    <i>Hydrangea macrophylla<\/i>\n                    inhibits glucose-induced lipid accumulation and reactive oxygen species generation in\n                    <i>Caenorhabditis elegans<\/i>"],"prefix":"10.1093","volume":"88","author":[{"given":"Myogyeong","family":"Cho","sequence":"first","affiliation":[{"name":"Department of Smart Food & Drug, Inje University , Gimhae , Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harin","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Digital Anti-aging Healthcare, Inje University , Gimhae , Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang Hyun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Smart Food & Drug, Inje University , Gimhae , Korea"},{"name":"Department of Food Technology and Nutrition, Inje University , Gimhae , Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6985-0957","authenticated-orcid":false,"given":"Myo-Jeong","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Smart Food & Drug, Inje University , Gimhae , Korea"},{"name":"Department of Digital Anti-aging Healthcare, Inje University , Gimhae , Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5934-982X","authenticated-orcid":false,"given":"Miran","family":"Jang","sequence":"additional","affiliation":[{"name":"Department of Smart Food & Drug, Inje University , Gimhae , Korea"},{"name":"Department of Digital Anti-aging Healthcare, Inje University , Gimhae , Korea"},{"name":"Department of Food Technology and Nutrition, Inje University , Gimhae , Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,4,10]]},"reference":[{"key":"2024062111405539100_bib1","first-page":"82","article-title":"Hepatoprotective role of Hydrangea macrophylla against sodium arsenite-induced mitochondrial-dependent oxidative stress via the inhibition of MAPK\/Caspase-3 pathways","volume":"1418","author":"Akanda","year":"2017","journal-title":"Int J Mol Sci"},{"key":"2024062111405539100_bib3","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/bs.vh.2022.09.002","article-title":"The antioxidant glutathione","volume":"121","author":"Averill-Bates","year":"2023","journal-title":"Vitam Horm"},{"key":"2024062111405539100_bib2","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1002\/biof.1666","article-title":"C. elegans ACAT regulates lipolysis and its related lifespan in fasting through modulation of the genes in lipolysis and insulin\/IGF-1 signaling","volume":"46","author":"Bai","year":"2020","journal-title":"Biofactors"},{"key":"2024062111405539100_bib4","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/j.etap.2012.12.012","article-title":"Water extract of processed Hydrangea macrophylla (Thunb.)Ser. leaf attenuates the expression of pro-inflammatory mediators by suppressing Akt-mediated NF-\u03bab activation","volume":"35","author":"Dilshara","year":"2013","journal-title":"Environ Toxicol Pharmacol"},{"key":"2024062111405539100_bib5","doi-asserted-by":"crossref","first-page":"1752","DOI":"10.1172\/JCI21625","article-title":"Increased oxidative stress in obesity and its impact on metabolic syndrome","volume":"114","author":"Furukawa","year":"2017","journal-title":"J Clin Invest"},{"key":"2024062111405539100_bib6","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1534\/genetics.115.174631","article-title":"Glucose induces sensitivity to oxygen deprivation and modulates insulin\/IGF-1 signaling and lipid biosynthesis in Caenorhabditis elegans","volume":"200","author":"Garcia","year":"2015","journal-title":"Genetics"},{"key":"2024062111405539100_bib7a","doi-asserted-by":"crossref","first-page":"104139","DOI":"10.1016\/j.jff.2020.104139","article-title":"Immune-enhancing activity of Hydrangea macrophylla subsp. serrata leaves through TLR4\/ROS-dependent activation of JNK and NF-\u03baB in RAW264.7 cells and immunosuppressed mice","volume":"73","author":"Geum","year":"2020","journal-title":"J Funct Food"},{"key":"2024062111405539100_bib8","doi-asserted-by":"crossref","first-page":"2672","DOI":"10.1039\/D0FO02185G","article-title":"Standardized hot water extract from the leaves of Hydrangea serrata (Thunb.) 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