{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T14:21:51Z","timestamp":1789222911477,"version":"build-2803163510"},"reference-count":23,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2024,5,11]],"date-time":"2024-05-11T00:00:00Z","timestamp":1715385600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institutions","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,6,21]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Polyethylene glycol modification (PEGylation) is a widely used strategy to improve the physicochemical properties of various macromolecules, especially protein drugs. However, its application in enhancing the performance of enzymes for molecular biology remains underexplored. This study explored the PEGylation of Bst DNA polymerase, determining optimal modification reaction conditions. In comparison to the unmodified wild-type counterpart, the modified Bst DNA polymerase exhibited significantly improved activity, thermal stability, and inhibitor tolerance during loop-mediated isothermal amplification. When applied for the detection of Salmonella in crude samples, the modified enzyme demonstrated a notably accelerated reaction rate. Therefore, PEGylation emerges as a viable strategy for refining DNA polymerases, helping in the development of novel molecular diagnostic reagents.<\/jats:p>","DOI":"10.1093\/bbb\/zbae059","type":"journal-article","created":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T05:25:18Z","timestamp":1715491518000},"page":"768-775","source":"Crossref","is-referenced-by-count":9,"title":["PEG modification increases thermostability and inhibitor resistance of Bst DNA polymerase"],"prefix":"10.1093","volume":"88","author":[{"given":"Mengxia","family":"Yang","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of Marine Biological Resources and Environment, Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Jiangsu Ocean University , Lianyungang , China"},{"name":"Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University , Lianyungang , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhixing","family":"Li","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Marine Biological Resources and Environment, Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Jiangsu Ocean University , Lianyungang , China"},{"name":"Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University , Lianyungang , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongjie","family":"Ren","sequence":"additional","affiliation":[{"name":"Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University , Lianyungang , China"},{"name":"Jiangsu BestEnzymes Biotech Co. 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