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Fustin is a flavanonol found in young fustic (Cotinus coggygria). However, little is known about its antimelanoma effects. Our study demonstrates that fustin suppresses the growth of B16 melanoma cells. Phalloidin staining of cytoskeletal actin revealed that fustin induced a conformational change in the actin structure of melanoma cells, accompanied by suppressed phosphorylation of myosin regulatory light chain 2 (MLC2), a regulator of actin structure. Furthermore, the protein kinase A (cAMP-dependent protein kinase) inhibitor H89 completely attenuated fustin-induced downregulation of phosphorylated myosin phosphatase targeting subunit 1, which is involved in dephosphorylation of MLC2. In a mouse model, administration of fustin suppressed tumor growth in B16 melanoma cells without adverse effects. In conclusion, our findings suggest that fustin effectively suppresses melanoma cell growth both in vitro and in vivo.<\/jats:p>","DOI":"10.1093\/bbb\/zbae072","type":"journal-article","created":{"date-parts":[[2024,6,5]],"date-time":"2024-06-05T04:20:59Z","timestamp":1717561259000},"page":"900-907","source":"Crossref","is-referenced-by-count":3,"title":["Fustin suppressed melanoma cell growth via cAMP\/PKA-dependent mechanism"],"prefix":"10.1093","volume":"88","author":[{"given":"Motofumi","family":"Kumazoe","sequence":"first","affiliation":[{"name":"Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University , Fukuoka , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshinori","family":"Fujimura","sequence":"additional","affiliation":[{"name":"Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University , Fukuoka , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Shimada","sequence":"additional","affiliation":[{"name":"Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University , Fukuoka , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroaki","family":"Onda","sequence":"additional","affiliation":[{"name":"Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University , Fukuoka , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yui","family":"Hatakeyama","sequence":"additional","affiliation":[{"name":"Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University , Fukuoka , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirofumi","family":"Tachibana","sequence":"additional","affiliation":[{"name":"Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University , Fukuoka , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,6,4]]},"reference":[{"key":"2024072211454575900_bib1","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/S1470-2045(22)00687-8","article-title":"Overall survival with first-line atezolizumab in combination with vemurafenib and cobimetinib in BRAFV600 mutation-positive advanced melanoma","volume":"24","author":"Ascierto","year":"2023","journal-title":"Lancet Oncol"},{"key":"2024072211454575900_bib2","doi-asserted-by":"crossref","first-page":"1569","DOI":"10.1002\/jbm.a.36670","article-title":"Modulating cancer cell mechanics and actin cytoskeleton structure by chemical and mechanical stimulations","volume":"107","author":"Azadi","year":"2019","journal-title":"J Biomedi Mater Res"},{"key":"2024072211454575900_bib3","doi-asserted-by":"crossref","first-page":"154970","DOI":"10.1016\/j.phymed.2023.154970","article-title":"67-kDa laminin receptor mediates oolonghomobisflavan B-induced cell growth inhibition in melanoma","volume":"118","author":"Bae","year":"2023","journal-title":"Phytomedicine"},{"key":"2024072211454575900_bib4","doi-asserted-by":"crossref","first-page":"1900986","DOI":"10.1002\/mnfr.201900986","article-title":"Procyanidin C1 inhibits melanoma cell growth by activating 67-kDa laminin receptor signaling","volume":"64","author":"Bae","year":"2020","journal-title":"Mol Nutr Food Res"},{"key":"2024072211454575900_bib5","doi-asserted-by":"crossref","first-page":"e93265","DOI":"10.1172\/jci.insight.93265","article-title":"Combination central tolerance and peripheral checkpoint blockade unleashes antimelanoma immunity","volume":"2","author":"Bakhru","year":"2017","journal-title":"JCI Insight"},{"key":"2024072211454575900_bib6","doi-asserted-by":"crossref","first-page":"E6240","DOI":"10.1073\/pnas.1703939114","article-title":"Analyses of PDE-regulated phosphoproteomes reveal unique and specific cAMP-signaling modules in T cells","volume":"114","author":"Beltejar","year":"2017","journal-title":"Proc Natl Acad. 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