{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T15:21:31Z","timestamp":1788016891313,"version":"build-2784847793"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T00:00:00Z","timestamp":1722556800000},"content-version":"vor","delay-in-days":1,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP20H02906"],"award-info":[{"award-number":["JP20H02906"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP20J12091"],"award-info":[{"award-number":["JP20J12091"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007802","name":"Institute for Fermentation, Osaka","doi-asserted-by":"publisher","award":["L-2020-3-021"],"award-info":[{"award-number":["L-2020-3-021"]}],"id":[{"id":"10.13039\/100007802","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,9,20]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Methanol reportedly stimulates citric acid (CA) production by Aspergillus niger and A. tubingensis; however, the underlying mechanisms remain unclear. Here, we elucidated the molecular functions of the citrate exporter gene cexA in relation to CA production by A. tubingensis WU-2223L. Methanol addition to the medium containing glucose as a carbon source markedly increased CA production by strain WU-2223L by 3.38-fold, resulting in a maximum yield of 65.5\u00a0g\/L, with enhanced cexA expression. Conversely, the cexA-complementing strain with the constitutive expression promoter Ptef1 (strain LhC-1) produced 68.3 or 66.7\u00a0g\/L of CA when cultivated without or with methanol, respectively. Additionally, strain LhC-2 harboring two copies of the cexA expression cassette produced 80.7\u00a0g\/L of CA without methanol addition. Overall, we showed that cexA is a target gene for methanol in CA hyperproduction by A. tubingensis WU-2223L. Based on these findings, methanol-independent CA-hyperproducing strains, LhC-1 and LhC-2, were successfully generated.<\/jats:p>","DOI":"10.1093\/bbb\/zbae099","type":"journal-article","created":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T01:15:23Z","timestamp":1722561323000},"page":"1203-1211","source":"Crossref","is-referenced-by-count":3,"title":["Generation of citric acid-hyperproducers independent of methanol effect by high-level expression of <i>cexA<\/i> encoding citrate exporter in <i>Aspergillus tubingensis<\/i>"],"prefix":"10.1093","volume":"88","author":[{"given":"Isato","family":"Yoshioka","sequence":"first","affiliation":[{"name":"R , Shinjuku, Tokyo , Japan"},{"name":"esearch Institute for Science and Engineering, Waseda University , Shinjuku, Tokyo , Japan"},{"name":"Medical Mycology Research Center, Chiba University , Chiba, Chiba , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4169-4486","authenticated-orcid":false,"given":"Kohtaro","family":"Kirimura","sequence":"additional","affiliation":[{"name":"R , Shinjuku, Tokyo , Japan"},{"name":"esearch Institute for Science and Engineering, Waseda University , Shinjuku, Tokyo , Japan"},{"name":"Department of Applied Chemistry, Faculty of Science and Engineering, Waseda University , Shinjuku, Tokyo , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,8,1]]},"reference":[{"key":"2024092012360026600_bib1","doi-asserted-by":"crossref","first-page":"e00446","DOI":"10.1128\/mSphere.00446-17","article-title":"A simple and universal system for gene manipulation in Aspergillus fumigatus: in vitro -assembled Cas9-guide RNA ribonucleoproteins coupled with microhomology repair templates. 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