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Although many bacterial strains possess HLDs or HLD homologues, LinB is the only HLD known to be involved in the utilization of \u03b3-hexachlorocyclohexane (\u03b3-HCH), a man-made chlorinated pesticide. In this study, to gain insight into the functional evolution of HLDs toward \u03b3-HCH utilization, the linB gene in \u03b3-HCH-degrading Sphingobium japonicum strain UT26 was replaced by 7 other HLD or HLD homologous genes, including 3 putative ancestral enzymes. Interestingly, strains carrying genes for DmmA from a marine metagenome or Rluc_anc, an ancestor of Renilla-luciferin 2-monooxygenase and LinB, produced 2,5-dichlorophenol and 2,5-dichlorohydroquinone from \u03b3-HCH, which are indicators of LinB activity, and grew in minimal medium supplied with \u03b3-HCH as a sole carbon source. These results indicated that other HLDs, in addition to LinB, can play roles in the \u03b3-HCH utilization.<\/jats:p>","DOI":"10.1093\/bbb\/zbaf022","type":"journal-article","created":{"date-parts":[[2025,3,5]],"date-time":"2025-03-05T02:30:07Z","timestamp":1741141807000},"page":"898-909","source":"Crossref","is-referenced-by-count":3,"title":["Haloalkane dehalogenases other than LinB can contribute to the \u03b3-hexachlorocyclohexane utilization"],"prefix":"10.1093","volume":"89","author":[{"given":"Nannan","family":"Chen","sequence":"first","affiliation":[{"name":"Tohoku University Department of Molecular and Chemical Life Sciences, , Sendai ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kouhei","family":"Kishida","sequence":"additional","affiliation":[{"name":"Tohoku University Department of Molecular and Chemical Life Sciences, , Sendai 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