{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T00:11:07Z","timestamp":1758672667704,"version":"3.44.0"},"reference-count":10,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T00:00:00Z","timestamp":1752710400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21H02126","24K01689"],"award-info":[{"award-number":["21H02126","24K01689"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,9,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Labor shortages threaten global apple production, thereby encouraging new strategies to improve orchard management. The growth of columnar apples, controlled by the MdDOX-Co gene, enables vertical growth with minimal lateral branching, allowing for high-density planting and easier harvesting. MdDOX-Co encodes 2-oxoglutarate-dependent dioxygenase (2ODD, DOX). This study aimed to identify selective chemical inhibitors of MdDOX-Co. We synthesized the parental C6-based analogs featuring a heterocyclic 1,3,4-oxathiazol-2-one ring and evaluated their inhibitory activity. Compounds retaining the 1,3,4-oxathiazol-2-one core exhibited strong in vitro inhibition and promoted seedling elongation in MdDOX-Co overexpressing Arabidopsis. Structure-activity analysis confirmed that the 1,3,4-oxathiazol-2-one ring was essential, with tolerance for side-chain variations, including bulky groups. Selectivity assays indicated minimal off-target effects on the related 2ODD enzymes. Molecular modeling suggested the compatibility of the lead compounds with the MdDOX-Co active site. These findings encourage us to develop MdDOX-Co-targeted agrochemicals to chemically regulate tree architecture and enhance productivity during apple cultivation.<\/jats:p>","DOI":"10.1093\/bbb\/zbaf103","type":"journal-article","created":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T15:29:42Z","timestamp":1752766182000},"page":"1456-1463","source":"Crossref","is-referenced-by-count":0,"title":["Shaping apple tree architecture: 1,3,4-oxathiazol-2-one derivatives as inhibitors of MdDOX-Co activity"],"prefix":"10.1093","volume":"89","author":[{"given":"Yuta","family":"Kitajima","sequence":"first","affiliation":[{"name":"Graduate School of Agricultural and Life Sciences, The University of Tokyo Department of Applied Biological Chemistry, , 1-1-1 Yayoi, Bunkyo-ku, Tokyo ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taiki","family":"Inoue","sequence":"additional","affiliation":[{"name":"Graduate School of Agricultural and Life Sciences, The University of Tokyo Department of Applied Biological Chemistry, , 1-1-1 Yayoi, Bunkyo-ku, Tokyo ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kojiro","family":"Kawada","sequence":"additional","affiliation":[{"name":"Graduate School of Agricultural and Life Sciences, The University of Tokyo Department of Applied Biological Chemistry, , 1-1-1 Yayoi, Bunkyo-ku, Tokyo ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7477-1674","authenticated-orcid":false,"given":"Tatsuo","family":"Saito","sequence":"additional","affiliation":[{"name":"Tokyo University of Agriculture Department of Chemistry for Agriculture and Life Sciences, Faculty of Life Science, , 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ikuo","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Graduate School of Agricultural and Life Sciences, The University of Tokyo Department of Applied Biological Chemistry, , 1-1-1 Yayoi, Bunkyo-ku, Tokyo ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kohji","family":"Murase","sequence":"additional","affiliation":[{"name":"Graduate School of Agricultural and Life Sciences, The University of Tokyo Department of Applied Biological Chemistry, , 1-1-1 Yayoi, Bunkyo-ku, Tokyo ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadao","family":"Asami","sequence":"additional","affiliation":[{"name":"Graduate School of Agricultural and Life Sciences, The University of Tokyo Department of Applied Biological Chemistry, , 1-1-1 Yayoi, Bunkyo-ku, Tokyo ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9679-341X","authenticated-orcid":false,"given":"Masatoshi","family":"Nakajima","sequence":"additional","affiliation":[{"name":"Graduate School of 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