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While the zoospores were significantly attracted to several amino acids, they did not respond to their typical nutrients such as sugars or organic acids, suggesting that the chemotaxis may not be directly involved in the search for their nutrition. Zoospores were also attracted to vanillin, which is a component of lignin and a major component of land plant cell walls. This suggests that Aurantiochytrium sp. may be involved in the cycling of carbons in terrestrial plant biomass between land and sea via lignin. Structural comparisons of responding and nonresponding vanilloids indicated that the hydrocarbon chain at position 1, the alkoxyl group at position 3, and the hydroxyl group at position 4 may be particularly important in vanilloid recognition.<\/jats:p>","DOI":"10.1093\/bbb\/zbaf151","type":"journal-article","created":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T12:27:10Z","timestamp":1761222430000},"page":"120-127","source":"Crossref","is-referenced-by-count":0,"title":["Chemotaxis of zoospores of\n                    <i>Aurantiochytrium limacinum<\/i>\n                    SR21"],"prefix":"10.1093","volume":"90","author":[{"given":"Kenshi","family":"Watanabe","sequence":"first","affiliation":[{"name":"Graduate School of Integrated Sciences for Life, Hiroshima University , Higashi-Hiroshima ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hikaru","family":"Tatsuta","sequence":"additional","affiliation":[{"name":"Graduate School of Integrated Sciences for Life, Hiroshima University , Higashi-Hiroshima ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moeko","family":"Arai","sequence":"additional","affiliation":[{"name":"Graduate School of Integrated Sciences for Life, Hiroshima University , Higashi-Hiroshima ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yusuke","family":"Hashima","sequence":"additional","affiliation":[{"name":"Graduate School of Integrated Sciences for Life, Hiroshima University , Higashi-Hiroshima ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsunehiro","family":"Aki","sequence":"additional","affiliation":[{"name":"Graduate School of Integrated Sciences for Life, Hiroshima University , Higashi-Hiroshima ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,10,24]]},"reference":[{"key":"2025121804020134400_bib1","doi-asserted-by":"publisher","first-page":"789","DOI":"10.1007\/s11746-003-0773-2","article-title":"Thraustochytrid as a potential source of carotenoids","volume":"80","author":"Aki","year":"2003","journal-title":"J Americ Oil Chem Soc"},{"key":"2025121804020134400_bib2","doi-asserted-by":"publisher","first-page":"9207","DOI":"10.1007\/s00253-014-5980-4","article-title":"Value-added lipid production from brown seaweed biomass by two-stage fermentation using acetic acid bacterium and thraustochytrid","volume":"98","author":"Arafiles","year":"2014","journal-title":"Appl Microbiol Biotechnol"},{"key":"2025121804020134400_bib3","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1007\/BF00029115","article-title":"Lower marine fungi (labyrinthulomycetes) and the decay of mangrove leaf litter","volume":"295","author":"Breamer","year":"1995","journal-title":"Hydrobiologia"},{"key":"2025121804020134400_bib4","doi-asserted-by":"publisher","first-page":"fuab038","DOI":"10.1093\/femsre\/fuab038","article-title":"Multiple functions of flagellar motility and chemotaxis in bacterial physiology","volume":"45","author":"Colin","year":"2021","journal-title":"FEMS Microbiol Rev"},{"key":"2025121804020134400_bib5","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1006\/fgbi.1999.1148","article-title":"Soybean isoflavones trigger a calcium influx in Phytophthora sojae","volume":"28","author":"Connolly","year":"1999","journal-title":"Fungal Genet Biol"},{"key":"2025121804020134400_bib6","doi-asserted-by":"publisher","first-page":"690","DOI":"10.1038\/nmeth.2075","article-title":"Icy: an open bioimage informatics platform for extended reproducible research","volume":"9","author":"De\u00a0Chaumont","year":"2012","journal-title":"Nat Methods"},{"key":"2025121804020134400_bib7","doi-asserted-by":"publisher","first-page":"16012","DOI":"10.1038\/s41598-017-16332-2","article-title":"The directed evolution of ligand specificity in a GPCR and the unequal contributions of efficacy and affinity","volume":"7","author":"Di\u00a0Roberto","year":"2017","journal-title":"Sci Rep"},{"key":"2025121804020134400_bib8","doi-asserted-by":"publisher","first-page":"0159","DOI":"10.1007\/S10267-002-0089-5","article-title":"Chemotactic and chemokinetic activities of Saprolegnia parasitica toward different metabolites and fish tissue extracts","volume":"44","author":"El-Feki","year":"2003","journal-title":"Mycoscience"},{"key":"2025121804020134400_bib9","doi-asserted-by":"publisher","first-page":"569","DOI":"10.1080\/15572536.2003.11833185","article-title":"Zoospore chemotaxis of mangrove thraustochytrids from Hong Kong","volume":"94","author":"Fan","year":"2002","journal-title":"Mycologia"},{"key":"2025121804020134400_bib10","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1016\/j.protis.2019.02.004","article-title":"Genomics, biology and phylogeny Aurantiochytrium acetophilum sp. nov. 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