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In this strain, propane is oxidized to 2-propanol by propane monooxygenase (Prm), and 2-propanol is further metabolized to methyl acetate, which is subsequently converted into acetate and methanol. However, methane and methanol metabolism in strain TY-5 has remained unclear. In this study, draft genome analysis revealed that strain TY-5 possesses the one-carbon (C1) dissimilation pathway, in which methanol is oxidized to CO2, but lacks a C1 assimilation pathway, suggesting that methanol is not utilized as the carbon source but is used as an energy source. Furthermore, we found that strain TY-5 can oxidize methane by Prm. Gene disruption analysis revealed that N, N-dimethyl-4-nitrosoaniline-dependent methanol dehydrogenase contributes to methanol oxidation in strain TY-5. Our findings provide new insights into the physiological significance of C1 and hydrocarbon metabolism for adapting the natural environment where methane and propane coexist.<\/jats:p>","DOI":"10.1093\/bbb\/zbaf185","type":"journal-article","created":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T13:05:47Z","timestamp":1765285547000},"page":"276-284","source":"Crossref","is-referenced-by-count":1,"title":["Methane oxidation and C1 metabolism in a propane-utilizer\n                    <i>Gordonia<\/i>\n                    sp. strain TY-5"],"prefix":"10.1093","volume":"90","author":[{"given":"Oktay","family":"Gafarov","sequence":"first","affiliation":[{"name":"Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University , Kitashirakawa-Oiwake, Sakyo-ku, Kyoto ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kohei","family":"Kida","sequence":"additional","affiliation":[{"name":"Division of Applied Life Sciences, Graduate 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