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Here, we identified and characterized CbGTS1 from the methylotrophic yeast Candida boidinii. Deletion of CbGTS1 reduced yeast proliferation on plant leaves and decreased tolerance to high-salt stress. These findings demonstrate that CbGts1 contributes to yeast adaptation and survival under challenging phyllosphere environments.<\/jats:p>","DOI":"10.1093\/bbb\/zbaf186","type":"journal-article","created":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T12:58:05Z","timestamp":1765630685000},"page":"438-442","source":"Crossref","is-referenced-by-count":0,"title":["Molecular characterization of\n                    <i>Candida boidinii GTS1<\/i>\n                    and its role in yeast proliferation in the phyllosphere"],"prefix":"10.1093","volume":"90","author":[{"given":"Kosuke","family":"Shiraishi","sequence":"first","affiliation":[{"name":"Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University , Sakyo-ku, Kyoto ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kana","family":"Shigeta","sequence":"additional","affiliation":[{"name":"Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University , Sakyo-ku, Kyoto ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Delia","family":"Saffian","sequence":"additional","affiliation":[{"name":"Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University , Sakyo-ku, Kyoto ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuyoshi","family":"Sakai","sequence":"additional","affiliation":[{"name":"Graduate School of Advanced Integrated Studies in Human Survivability, Kyoto University \u00a0 , Sakyo-ku, Kyoto ,","place":["Japan"]},{"name":"Kyoto University of Advanced Science Department of Applied Biological Sciences, Faculty of Bioenvironmental Sciences, , 1-1 Sogabecho Nanjo Otani, Kameoka ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7506-6184","authenticated-orcid":false,"given":"Hiroya","family":"Yurimoto","sequence":"additional","affiliation":[{"name":"Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University , Sakyo-ku, Kyoto ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,12,15]]},"reference":[{"key":"2026022401213919500_bib1","doi-asserted-by":"publisher","first-page":"6236","DOI":"10.1038\/s41467-023-41589-9","article-title":"Green leaf volatile sensory calcium transduction in Arabidopsis","volume":"14","author":"Aratani","year":"2023","journal-title":"Nat Commun"},{"key":"2026022401213919500_bib2","doi-asserted-by":"publisher","first-page":"108508","DOI":"10.1016\/j.ijfoodmicro.2020.108508","article-title":"Bacterial and fungal diversity in Laphet, traditional fermented tea leaves in Myanmar, analyzed by culturing, DNA amplicon-based sequencing, and PCR-DGGE methods","volume":"320","author":"Bo","year":"2020","journal-title":"Int J Food Microbiol"},{"key":"2026022401213919500_bib3","doi-asserted-by":"publisher","first-page":"422","DOI":"10.3389\/fpls.2013.00422","article-title":"\u201cBreath figures\u201d on leaf surfaces-formation and effects of microscopic leaf wetness","volume":"4","author":"Burkhardt","year":"2013","journal-title":"Front Plant Sci"},{"key":"2026022401213919500_bib4","doi-asserted-by":"publisher","first-page":"16428","DOI":"10.1073\/pnas.0905240106","article-title":"Community proteogenomics reveals insights into the physiology of phyllosphere bacteria","volume":"106","author":"Delmotte","year":"2009","journal-title":"Proc Natl Acad Sci USA"},{"key":"2026022401213919500_bib5","doi-asserted-by":"publisher","first-page":"296","DOI":"10.1016\/S1360-1385(96)88175-0","article-title":"Leaf methanol\u2014the simplest natural product from plants","volume":"1","author":"Fall","year":"1996","journal-title":"Trends Plant Sci"},{"key":"2026022401213919500_bib6","doi-asserted-by":"publisher","first-page":"1027","DOI":"10.1128\/jb.01483-07","article-title":"PhyR is involved in the general stress response of Methylobacterium extorquens AM1","volume":"190","author":"Gourion","year":"2008","journal-title":"J Bacteriol"},{"key":"2026022401213919500_bib7","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1007\/s00203-013-0922-6","article-title":"Stress resistance and C1 metabolism involved in plant colonization of a methanotroph Methylosinus sp. 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