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The nonmotile Sphingomonas sp. strain A1 imports alginate, a major component of brown algae, by alginate-binding proteins AlgQ1\/Q2 and an ATP-binding cassette transporter. Previously, we showed that metabolically engineered ethanol-fermentative strains A1 (A1-EPv14 and A1-EPv104) produced bioethanol from alginate and that repeated subculturing of strain A1 on soft agar plates inducibly activated motility, resulting in expression of chemotaxis toward alginate. This study examined the possibility of bioethanol production directly from brown algae, and what triggers chemotaxis and how chemotaxis affects ethanol yield. Strain A1-EPv104 produced ethanol from brown algae as well as alginate. Gene disruption and complementation revealed that AlgQ2, not AlgQ1, was involved in alginate chemotaxis. Compared with nonmotile strain A1-EPv14, strain A1-EPv14c exhibiting alginate chemotaxis produced ethanol from alginate more rapidly. This is the first report on chemotaxis-associated biotechnology for the rapid production of bioethanol from blue carbon alginate.<\/jats:p>","DOI":"10.1093\/bbb\/zbag001","type":"journal-article","created":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T12:29:52Z","timestamp":1767356992000},"page":"414-421","source":"Crossref","is-referenced-by-count":0,"title":["Chemotaxis-associated biotechnology for the bacterial production of biofuel using blue carbon alginate"],"prefix":"10.1093","volume":"90","author":[{"given":"Mamoru","family":"Hio","sequence":"first","affiliation":[{"name":"Laboratory of Basic and Applied Molecular Biotechnology, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryuichi","family":"Takase","sequence":"additional","affiliation":[{"name":"Laboratory of Basic and Applied Molecular Biotechnology, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kohei","family":"Ogura","sequence":"additional","affiliation":[{"name":"Laboratory of Basic and Applied Molecular Biotechnology, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0185-2371","authenticated-orcid":false,"given":"Wataru","family":"Hashimoto","sequence":"additional","affiliation":[{"name":"Laboratory of Basic and Applied Molecular Biotechnology, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2026,1,5]]},"reference":[{"key":"2026022401214838100_bib1","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J Mol Biol"},{"key":"2026022401214838100_bib2","doi-asserted-by":"publisher","first-page":"12653","DOI":"10.1038\/s41598-022-16540-5","article-title":"Substrate size-dependent conformational changes of bacterial pectin-binding protein crucial for chemotaxis and assimilation","volume":"12","author":"Anamizu","year":"2022","journal-title":"Sci Rep"},{"key":"2026022401214838100_bib4","doi-asserted-by":"publisher","first-page":"1242","DOI":"10.1038\/s41467-025-56410-y","article-title":"An atlas of metabolites driving chemotaxis in prokaryotes","volume":"16","author":"Brunet","year":"2025","journal-title":"Nat Commun"},{"key":"2026022401214838100_bib5","doi-asserted-by":"publisher","first-page":"1823","DOI":"10.1111\/1751-7915.14292","article-title":"Sensing preferences for prokaryotic solute binding protein families","volume":"16","author":"Cerna-Vargas","year":"2023","journal-title":"Microb Biotechnol"},{"key":"2026022401214838100_bib6","doi-asserted-by":"publisher","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"fastp: an ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"2026022401214838100_bib7","doi-asserted-by":"crossref","unstructured":"Danecek P, Bonfield JK, Liddle J \u00a0et al. \u00a0Twelve years of SAMtools and BCFtools. 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