{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T15:38:30Z","timestamp":1774021110271,"version":"3.50.1"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100007834","name":"Ningbo Natural Science Foundation","doi-asserted-by":"publisher","award":["2023J323"],"award-info":[{"award-number":["2023J323"]}],"id":[{"id":"10.13039\/100007834","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Zhu Xiu Shan Talent Project of Ningbo No. 2 Hospital","award":["2023HMJQ34"],"award-info":[{"award-number":["2023HMJQ34"]}]},{"name":"Zhejiang Province Key Clinical Specialty Construction Project","award":["2024023"],"award-info":[{"award-number":["2024023"]}]},{"name":"Ningbo Medical & Health Brand Discipline","award":["PPXK2024-04"],"award-info":[{"award-number":["PPXK2024-04"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Thrombopoietin (TPO) is essential for treating thrombocytopenia, but its clinical use is limited by immunogenicity and short half-life. TMP, a TPO mimetic peptide, addresses these issues but requires fusion with carriers to improve pharmacokinetics. This study developed ELP-TMP fusion proteins (ELP120-2TMP and 2TMP-ELP120) to extend half-life and enhance activity via elastin-like polypeptide (ELP). Results showed EC50 values of 5.81\u00a0n\u2009m for ELP120-2TMP and 10.88\u00a0n\u2009m for 2TMP-ELP120, compared to 2.65\u00a0n\u2009m for recombinant human TPO (rhTPO). At a dose of 600\u00a0nmol\/kg, ELP120-2TMP resulted in peak platelet counts in mice on day 20, exhibiting a half-life of 22.9\u00a0h. Conversely, 2TMP-ELP120 achieved peak platelet counts on day 12, with a half-life of 25.4\u00a0h. The half-lives of both fusion proteins were significantly longer than that reported 2TMP alone (1\u00a0h). Area under the curve indicated superior platelet stimulation over rhTPO (P\u00a0&amp;lt;\u00a00.01).<\/jats:p>","DOI":"10.1093\/bbb\/zbag014","type":"journal-article","created":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T12:43:44Z","timestamp":1769863424000},"page":"535-542","source":"Crossref","is-referenced-by-count":0,"title":["Elastin-like polypeptide enhances the therapeutic activity of thrombopoietin mimetic peptide"],"prefix":"10.1093","volume":"90","author":[{"given":"Xiansheng","family":"Zhao","sequence":"first","affiliation":[{"name":"Ningbo No. 2 Hospital Department of Liver Disease, , Ningbo ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yutuo","family":"Zheng","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital of Wenzhou Medical University Department of Neurosurgery, , Wenzhou 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