{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T01:51:38Z","timestamp":1789609898128,"version":"build-2803163510"},"reference-count":16,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T00:00:00Z","timestamp":1770249600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001863","name":"New Energy and Industrial Technology Development Organization","doi-asserted-by":"publisher","award":["P20011"],"award-info":[{"award-number":["P20011"]}],"id":[{"id":"10.13039\/501100001863","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,4,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Peptone is a promising raw material for culturing microorganisms and mammalian cells, but its composition remains unclear. Here, 36 different peptones were comprehensively quantified using five approaches: gas chromatography\/tandem mass spectrometry, liquid chromatography\/mass spectrometry, ion chromatography, postcolumn detection\u2013high-performance liquid chromatography, and inductively coupled plasma mass spectrometry (named multimodal targeting analysis). Seventy-eight chemical compounds\/ions were detected among 121 targets, including amino acids, nucleic acids, organic acids, sugars, vitamins, and minerals. The sum of the quantitated component weights exceeded 65% of the total weight in all cases (mean 88%). Principal component and cluster dendrogram analyses revealed clear distinctions between not only peptone brands but also production lots. Plant peptones exhibited greater variety than casein and meat peptones. Partial least squares analysis identified components specific to particular manufacturing processes and peptone sources. Acid-digested peptones contained more free amino acids, including Ala, Cys, Gly, Thr, Ser, Asp, Glu, and Pro, than enzyme-digested types.<\/jats:p>","DOI":"10.1093\/bbb\/zbag023","type":"journal-article","created":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T12:43:59Z","timestamp":1770209039000},"page":"702-710","source":"Crossref","is-referenced-by-count":1,"title":["High-resolution profiling for the composition of various peptones by multimodal targeting analysis"],"prefix":"10.1093","volume":"90","author":[{"given":"Takuto","family":"Nakajima","sequence":"first","affiliation":[{"name":"Graduate School of Engineering, Kitami Institute of Technology , Kitami, Hokkaido ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuki","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Graduate School of Engineering, Kitami Institute of Technology , Kitami, Hokkaido ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoko","family":"Kagenishi","sequence":"additional","affiliation":[{"name":"Graduate School of Engineering, Kitami Institute of Technology , Kitami, Hokkaido ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0616-073X","authenticated-orcid":false,"given":"Masaaki","family":"Konishi","sequence":"additional","affiliation":[{"name":"Kitami Institute of Technology Department of Applied Chemistry, , Kitami, Hokkaido ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,2,5]]},"reference":[{"key":"2026042213030771800_bib1","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1111\/j.1432-1033.1978.tb12574.x","article-title":"The composition, structure and origin of proteose-peptone component 5 of bovine milk","volume":"90","author":"Andrews","year":"1978","journal-title":"Eur J Biochem"},{"key":"2026042213030771800_bib7","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1016\/j.procbio.2020.12.004","article-title":"Farmed salmon rest raw materials as a source of peptones for industrial fermentation media","volume":"102","author":"Broli","year":"2021","journal-title":"Proc Biochem"},{"key":"2026042213030771800_bib2","doi-asserted-by":"publisher","first-page":"557","DOI":"10.1016\/j.tifs.2009.07.002","article-title":"Metabolomic analysis in food science:a review","volume":"20","author":"Cevallos-Cevallos","year":"2009","journal-title":"Trends Food Sci Technol"},{"key":"2026042213030771800_bib3","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1016\/j.jchromb.2014.03.025","article-title":"Analysis of amino acid composition in proteins of animal tissues and foods as pre-column o-phthaldialdehyde derivatives by HPLC with fluorescence detection","volume":"964","author":"Dai","year":"2014","journal-title":"J Chromatogr B"},{"key":"2026042213030771800_bib4","doi-asserted-by":"publisher","first-page":"194","DOI":"10.7508\/ibj.2015.04.002","article-title":"Effects of peptone supplementation in different culture media on growth, metabolic pathway and productivity of CHO DG44 cells; 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