{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T03:48:42Z","timestamp":1782186522488,"version":"3.54.5"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T00:00:00Z","timestamp":1776729600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Clinical Medicine Special Fund Project of Zhejiang Medical Association","award":["2024ZYC-Z16"],"award-info":[{"award-number":["2024ZYC-Z16"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>This study investigated the mechanism of pirfenidone (PFD) inhibiting the metastasis of lung cancer in vivo. A nude mouse lung cancer model was established and treated with PFD (200 and 400 mg\/kg); lung metastatic tumor weight was recorded, Lyve-1 (lymphatic marker), LC3I and LC3II (autophagy protein), and polo-like kinase 1 (PLK1) were detected. In human lung lymphatic endothelial cells (HMVEC-LLy), PFD\u2019s effects on migration, apoptosis, lymphangiogenesis, and LC3II\/LC3I\/PLK1 levels were tested. PLK1-overexpressed HMVEC-LLy cells were treated with 400 \u03bcg\/mL PFD to explore its mechanism. PFD inhibited the weight of lung metastases and downregulated the expression levels of Lyve-1, LC3II\/LC3I, and PLK1. In cell experiments, PFD was found to inhibit HMVEC-LLy migration and lymph angiogenesis, and promote apoptosis, as well as downregulate LC3II\/LC3I and PLK1 protein levels, and these effects could be reversed by PLK1 overexpression. PFD may inhibit lung cancer metastasis in vivo by regulating lymphangiogenesis through PLK1 signaling.<\/jats:p>","DOI":"10.1093\/bbb\/zbag054","type":"journal-article","created":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T11:21:28Z","timestamp":1776684088000},"page":"877-885","source":"Crossref","is-referenced-by-count":0,"title":["Effect of pirfenidone on the regulation of lymph angiogenesis by PLK1 signaling to inhibit metastasis of lung cancer\n                    <i>in vivo<\/i>"],"prefix":"10.1093","volume":"90","author":[{"given":"Zehai","family":"Xia","sequence":"first","affiliation":[{"name":"The Affiliated Hospital of Hangzhou Normal University Department of Pulmonary and Critical Care Medicine, , Hangzhou, Zhejiang ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quan","family":"Wang","sequence":"additional","affiliation":[{"name":"Clinical Medical College, Hangzhou Normal University , Hangzhou, Zhejiang 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