{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T09:52:40Z","timestamp":1791193960673,"version":"4.1.0"},"reference-count":41,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T00:00:00Z","timestamp":1787961600000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100009027","name":"Advanced Low Carbon Technology Research and Development Program","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009027","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24K01894"],"award-info":[{"award-number":["24K01894"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>A simplified synthetic nitrifying microbial community capable of sequential ammonification and nitrification under organic conditions was constructed using three defined strains: Bacillus badius NBRC 15713, Nitrosomonas europaea NBRC 14298, and Nitrobacter winogradskyi NBRC 14297. This co-culture achieved complete ammonification and nitrification under selected organic nitrogen conditions, with NO\u2083\u207b\u2013N accumulation of approximately 56 mg\u2013N\/L and no residual NO\u2082\u207b\u2013N detected. RT-qPCR analysis showed that changes in the relative abundance of N. europaea and N. winogradskyi were associated with changes in inorganic nitrogen concentrations during cultivation, indicating that the co-culture supported the growth and activity of nitrifying bacteria despite the presence of organic compounds that inhibit nitrification. This defined three-strain community provides a reproducible model for studying sequential microbial nitrogen conversion under organic nitrogen conditions.<\/jats:p>","DOI":"10.1093\/bbb\/zbag131","type":"journal-article","created":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:08:33Z","timestamp":1788048513000},"source":"Crossref","is-referenced-by-count":0,"title":["Development of a defined three-strain nitrifying microbial community for ammonification and nitrification under organic nitrogen conditions"],"prefix":"10.1093","author":[{"given":"Htun","family":"Inzali","sequence":"first","affiliation":[{"name":"Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University , Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sakuntala","family":"Saijai","sequence":"additional","affiliation":[{"name":"Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University , Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wakako","family":"Okada","sequence":"additional","affiliation":[{"name":"Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University , Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Ogawa","sequence":"additional","affiliation":[{"name":"Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University , Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto ,","place":["Japan"]},{"name":"Research Unit for Physiological Chemistry, Kyoto University , Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akinori","family":"Ando","sequence":"additional","affiliation":[{"name":"Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University , 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