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This study evaluated digestion-dependent changes in IgE reactivity and the in vivo behavior of PA from salmon, flounder, and herring.\u00a0In vitro, PA was rapidly cleaved by pepsin\u2013trypsin, but the resulting low-molecular-weight fragments retained substantial IgE reactivity. In mice, orally administered PA was also digested in the gastrointestinal tract, although part of the protein persisted in the small intestine for extended periods. Notably, PA traversed the cytoplasm of intestinal epithelial cells and rapidly appeared in the venous bloodstream, indicating transcellular absorption. These findings were consistent across the three species, suggesting that the persistence of PA\u2019s IgE reactivity despite extensive digestive fragmentation, together with its sustained intestinal uptake, is associated with its strong allergenic potential. This study provides key insights into the mechanisms linking digestive and absorptive processes to PA allergenicity.<\/jats:p>","DOI":"10.1093\/bbb\/zbag140","type":"journal-article","created":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T11:43:44Z","timestamp":1789213424000},"source":"Crossref","is-referenced-by-count":0,"title":["The digestive fate and intestinal transport pathway of parvalbumin underlying its strong allergenicity"],"prefix":"10.1093","author":[{"given":"Nobuhiro","family":"Sudo","sequence":"first","affiliation":[{"name":"Faculty of Fisheries Sciences, Hokkaido University , Minato 3, Hakodate, Hokkaido ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ga-Hyun","family":"Joe","sequence":"additional","affiliation":[{"name":"Faculty of Fisheries Sciences, Hokkaido University , Minato 3, Hakodate, Hokkaido 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