{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T17:03:27Z","timestamp":1790269407606,"version":"4.1.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Quinuclidine derivatives are promising pharmaceutical agents. Herein, we screened for microorganisms capable of converting 3-formylquinuclidine and found that Brevibacterium aurantiacum NBRC 12171 converted 3-formylquinuclidine into quinuclidin-3-ylmethanamine. Through enzyme purification and the heterologous expression of candidate genes, the L-alanine-3-formylquinuclidine aminotransferase gene was identified. Using purified recombinant L-alanine-3-formylquinuclidine aminotransferase, we identified L-alanine as an amino donor for the conversion of 3-formylquinuclidine to quinuclidin-3-ylmethanamine. These findings provide a potential biocatalytic approach for the sustainable production of quinuclidine-based pharmaceutical building blocks.<\/jats:p>","DOI":"10.1093\/bbb\/zbag143","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T11:52:33Z","timestamp":1790164353000},"source":"Crossref","is-referenced-by-count":0,"title":["Gene identification of L-alanine-3-formylquinuclidine aminotransferase from\n                    <i>Brevibacterium aurantiacum<\/i>\n                    NBRC 12171"],"prefix":"10.1093","author":[{"given":"Yuki","family":"Okumura","sequence":"first","affiliation":[{"name":"Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University , Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Jomen","sequence":"additional","affiliation":[{"name":"Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University , Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michiki","family":"Takeuchi","sequence":"additional","affiliation":[{"name":"Faculty of Molecular Chemistry and Engineering, Kyoto Institute of Technology , Hashikami-cho, Matsugasaki, Sakyo-ku, Kyoto ,","place":["Japan"]},{"name":"Industrial Microbiology, Graduate School of Agriculture, Kyoto University , Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoko","family":"Kozono","sequence":"additional","affiliation":[{"name":"Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University , Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryotaro","family":"Hara","sequence":"additional","affiliation":[{"name":"Industrial Microbiology, Graduate School of Agriculture, Kyoto University , Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomotsune","family":"Onaga","sequence":"additional","affiliation":[{"name":"Yuki Gosei Kogyo Co., Ltd. , 10-4, Nihonbashi-Ningyocho 3-chome, Chuo-ku, Tokyo ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuji","family":"Noda","sequence":"additional","affiliation":[{"name":"Yuki Gosei Kogyo Co., Ltd. , 10-4, Nihonbashi-Ningyocho 3-chome, Chuo-ku, Tokyo ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seiichiro","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Yuki Gosei Kogyo Co., Ltd. , 10-4, Nihonbashi-Ningyocho 3-chome, Chuo-ku, Tokyo ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Ueda","sequence":"additional","affiliation":[{"name":"Industrial Microbiology, Graduate School of Agriculture, Kyoto University , Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Ogawa","sequence":"additional","affiliation":[{"name":"Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University , Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,24]]},"container-title":["Bioscience, Biotechnology, and Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/bbb\/advance-article-pdf\/doi\/10.1093\/bbb\/zbag143\/71382484\/zbag143.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bbb\/advance-article-pdf\/doi\/10.1093\/bbb\/zbag143\/71382484\/zbag143.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T16:22:43Z","timestamp":1790266963000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bbb\/advance-article\/doi\/10.1093\/bbb\/zbag143\/8833855"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,24]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1093\/bbb\/zbag143","relation":{},"ISSN":["1347-6947"],"issn-type":[{"value":"1347-6947","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,24]]},"article-number":"zbag143"}}