{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T22:39:03Z","timestamp":1792795143928,"version":"4.5.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T00:00:00Z","timestamp":1792713600000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Plant growth-promoting bacteria are gaining significant attention as promising biofertilizers. However, the inconsistency between in vitro plant growth-promoting traits and actual field performance remains a challenge, driven partly by a limited understanding of in situ colonization. This study characterized the colonization patterns of Citrobacter sedlakii CESi7, a novel plant growth-promoting bacterium, isolated from oil palm waste compost, during Brassica rapa cultivation. The in situ behavior of CESi7 was observed in both sterilized medium and non-sterilized soil using fluorescence in situ hybridization with a strain-targeting probe. The results revealed that CESi7 can establish both epiphytic and endophytic populations that transiently colonize roots. In a sterilized medium, CESi7 was widely distributed throughout the root tissues. Conversely, in non-sterilized soil, the bacterium formed dense aggregates specifically at the root tips. This study provides direct microscopic evidence of the colonization strategy of CESi7, offering crucial insights for its development as an effective biofertilizer.<\/jats:p>","DOI":"10.1093\/bbb\/zbag158","type":"journal-article","created":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T11:39:31Z","timestamp":1792669171000},"source":"Crossref","is-referenced-by-count":0,"title":["Fluorescence\n                    <i>in situ<\/i>\n                    hybridization reveals endophytic and epiphytic root colonization of the novel plant growth-promoting bacterium\n                    <i>Citrobacter sedlakii<\/i>\n                    CESi7"],"prefix":"10.1093","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-9778-4786","authenticated-orcid":false,"given":"Hiromu","family":"Inoue","sequence":"first","affiliation":[{"name":"Faculty of Agriculture, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University Laboratory of Soil and Environmental Microbiology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, , Fukuoka ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manami","family":"Maeda","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University Laboratory of Soil and Environmental Microbiology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, , Fukuoka ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomonori","family":"Koga","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University Laboratory of Soil and Environmental Microbiology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, , Fukuoka ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5988-5033","authenticated-orcid":false,"given":"Zahra","family":"Salman","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University Laboratory of Soil and Environmental Microbiology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, , Fukuoka ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clament Fui Seung","family":"Chin","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Faculty of Sustainable Agriculture, Universiti Malaysia Sabah , Sabah ,","place":["Malaysia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7900-9678","authenticated-orcid":false,"given":"Mohd Huzairi","family":"Mohd Zainudin","sequence":"additional","affiliation":[{"name":"Laboratory of Sustainable Animal Production and Biodiversity, Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia , Selangor ,","place":["Malaysia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8015-8654","authenticated-orcid":false,"given":"Norhayati Bt","family":"Ramli","sequence":"additional","affiliation":[{"name":"Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia Environmental Biotechnology Research Group, Department of Bioprocess Technology, , Selangor ,","place":["Malaysia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohd Ali","family":"Hassan","sequence":"additional","affiliation":[{"name":"Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia Environmental Biotechnology Research Group, Department of Bioprocess Technology, , Selangor ,","place":["Malaysia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3245-7227","authenticated-orcid":false,"given":"Yukihiro","family":"Tashiro","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University Laboratory of Soil and Environmental Microbiology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, , Fukuoka ,","place":["Japan"]},{"name":"Laboratory of Microbial Environmental Protection, Tropical Microbiology Unit, Center for International Education and Research of Agriculture, Faculty of Agriculture, Kyushu University , Fukuoka ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji","family":"Sakai","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University Laboratory of Soil and Environmental Microbiology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, , Fukuoka ,","place":["Japan"]},{"name":"Laboratory of Microbial Environmental Protection, Tropical Microbiology Unit, Center for International Education and Research of Agriculture, Faculty of Agriculture, Kyushu University , Fukuoka ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,10,23]]},"container-title":["Bioscience, Biotechnology, and Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/bbb\/advance-article-pdf\/doi\/10.1093\/bbb\/zbag158\/72447270\/zbag158.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bbb\/advance-article-pdf\/doi\/10.1093\/bbb\/zbag158\/72447270\/zbag158.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:37:03Z","timestamp":1792791423000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bbb\/advance-article\/doi\/10.1093\/bbb\/zbag158\/8994303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,23]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1093\/bbb\/zbag158","relation":{},"ISSN":["1347-6947"],"issn-type":[{"value":"1347-6947","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,23]]},"article-number":"zbag158"}}