{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T14:22:24Z","timestamp":1792160544082,"version":"4.4.0"},"reference-count":76,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2024,8,12]],"date-time":"2024-08-12T00:00:00Z","timestamp":1723420800000},"content-version":"vor","delay-in-days":18,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,7,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Pseudomonas aeruginosa is a complex nosocomial infectious agent responsible for numerous illnesses, with its growing resistance variations complicating treatment development. Studies have emphasized the importance of virulence factors OprE and OprF in pathogenesis, highlighting their potential as vaccine candidates. In this study, B-cell, MHC-I, and MHC-II epitopes were identified, and molecular linkers were active to join these epitopes with an appropriate adjuvant to construct a vaccine. Computational tools were employed to forecast the tertiary framework, characteristics, and also to confirm the vaccine\u2019s composition. The potency was weighed through population coverage analysis and immune simulation. This project aims to create a multi-epitope vaccine to reduce P. aeruginosa\u2013related illness and mortality using immunoinformatics resources. The ultimate complex has been determined to be stable, soluble, antigenic, and non-allergenic upon inspection of its physicochemical and immunological properties. Additionally, the protein exhibited acidic and hydrophilic characteristics. The Ramachandran plot, ProSA-web, ERRAT, and Verify3D were employed to ensure the final model\u2019s authenticity once the protein\u2019s three-dimensional structure had been established and refined. The vaccine model showed a significant binding score and stability when interacting with MHC receptors. Population coverage analysis indicated a global coverage rate of 83.40%, with the USA having the highest coverage rate, exceeding 90%. Moreover, the vaccine sequence underwent codon optimization before being cloned into the Escherichia coli plasmid vector pET-28a (+) at the EcoRI and EcoRV restriction sites. Our research has developed a vaccine against P. aeruginosa that has strong binding affinity and worldwide coverage, offering an acceptable way to mitigate nosocomial infections.<\/jats:p>","DOI":"10.1093\/bib\/bbae401","type":"journal-article","created":{"date-parts":[[2024,8,12]],"date-time":"2024-08-12T12:26:33Z","timestamp":1723465593000},"source":"Crossref","is-referenced-by-count":20,"title":["A computational approach to developing a multi-epitope vaccine for combating <i>Pseudomonas aeruginosa<\/i>\u2013induced pneumonia and sepsis"],"prefix":"10.1093","volume":"25","author":[{"given":"Suronjit Kumar","family":"Roy","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Biotechnology, Khwaja Yunus Ali University , Sirajganj 6751, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1545-5521","authenticated-orcid":false,"given":"Mohammad Shahangir","family":"Biswas","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biotechnology, Khwaja Yunus Ali University , Sirajganj 6751, Bangladesh"},{"name":"Department of Public Health, Daffodil International University , Dhaka 1216, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md","family":"Foyzur Raman","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biotechnology, Khwaja Yunus Ali University , Sirajganj 6751, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rubait","family":"Hasan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biotechnology, Khwaja Yunus Ali University , Sirajganj 6751, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahidur","family":"Rahmann","sequence":"additional","affiliation":[{"name":"Institute of Biological Science, Rajshahi University , Motihar, Rajshahi 6205, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md Moyen","family":"Uddin PK","sequence":"additional","affiliation":[{"name":"Riceland Healthcare , 538 Broadway Ave, Winnie, TX 77665, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,8,12]]},"reference":[{"key":"2024081906315601700_ref1","first-page":"e0189621","article-title":"Clinical and economic impact of antibiotic resistance in developing countries: a systematic review and meta-analysis","author":"Founou","year":"2017"},{"key":"2024081906315601700_ref2","doi-asserted-by":"crossref","first-page":"403107","DOI":"10.3389\/fmicb.2019.00539","article-title":"Emerging strategies to combat ESKAPE pathogens in the era of antimicrobial resistance: a review","volume":"10","author":"Mulani","year":"2019","journal-title":"Front Microbiol"},{"key":"2024081906315601700_ref3","article-title":"Articles discovery, research, and development of 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