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Optimizing mRNA-based antigen expression is a crucial focus in this emerging industry. We have developed a bioinformatics tool named AntigenBoost to address the challenge posed by destabilizing dipeptides that hinder ribosomal translation. AntigenBoost identifies these dipeptides within specific antigens and provides a range of potential amino acid substitution strategies using a two-dimensional scoring system. Through a combination of bioinformatics analysis and experimental validation, we significantly enhanced the in vitro expression of mRNA-derived Respiratory Syncytial Virus fusion glycoprotein and Influenza A Hemagglutinin antigen. Notably, a single amino acid substitution improved the immune response in mice, underscoring the effectiveness of AntigenBoost in mRNA vaccine design.<\/jats:p>","DOI":"10.1093\/bib\/bbae468","type":"journal-article","created":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T11:33:57Z","timestamp":1728905637000},"source":"Crossref","is-referenced-by-count":2,"title":["AntigenBoost: enhanced mRNA-based antigen expression through rational amino acid substitution"],"prefix":"10.1093","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-0874-0085","authenticated-orcid":false,"given":"Yumiao","family":"Gao","sequence":"first","affiliation":[{"name":"NanoRibo (Shanghai) Biotechnology Co., Ltd. , No. 1188 Lianhang Road, Minhang District, Shanghai 200003 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siran","family":"Zhu","sequence":"additional","affiliation":[{"name":"NanoRibo (Shanghai) Biotechnology Co., Ltd. , No. 1188 Lianhang Road, 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