{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T12:33:55Z","timestamp":1786365235096,"version":"build-2736575974"},"reference-count":66,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T00:00:00Z","timestamp":1777248000000},"content-version":"vor","delay-in-days":57,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"NextGenEU programme \u201cArtificial Intelligence and Other Digital Technologies to Drive Their Integration into Value Chains\u201d","award":["NextGenEU-C005\/21-ED 2021\/C005\/00149851"],"award-info":[{"award-number":["NextGenEU-C005\/21-ED 2021\/C005\/00149851"]}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011033","name":"Agencia Estatal de Investigaci\u00f3n","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011033","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ayudas para Doctorados Industriales","award":["DIN 2022-012817 to F.A."],"award-info":[{"award-number":["DIN 2022-012817 to F.A."]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Artificial intelligence (AI) integrated with high-throughput assays offers a powerful route to accelerate discovery in relevant biological models. Functional cardiac imaging is a prime application, where deep learning (DL) and explainable AI (xAI) can overcome limitations of traditional phenotyping methods, such as manual analysis, subjective interpretation, and low scalability. In cardiovascular research, the zebrafish model is highly valuable due to its translational relevance and accessibility for high-throughput applications. Here, we present ZeCardioAI, a computational platform combining zebrafish experimental advantages with DL and xAI methodologies. The platform automatically extracts comprehensive cardiac phenotypes from live imaging, achieving high precision while maintaining interpretability, critical for mechanistic insight and translational validation. ZeCardioAI, when applied to zebrafish models of dilated and hypertrophic cardiomyopathy (CM), detected subtle yet clinically relevant phenotypic differences. Machine learning classifiers achieved robust separation of disease from healthy phenotypes, and xAI revealed discriminative features aligning with established clinical markers. Our developments should prove valuable in addressing the unmet medical need in CMs to find new, specific treatments. The platform\u2019s modular architecture supports future adaptation to diverse disease contexts beyond CMs, enabling large-scale, fully automated phenotyping at a throughput unattainable by manual approaches. ZeCardioAI establishes a new standard for AI-powered biological research, offering transformative potential for accelerating drug discovery, advancing precision medicine approaches, and deepening fundamental understanding of complex biological systems across multiple therapeutic areas.<\/jats:p>","DOI":"10.1093\/bib\/bbag197","type":"journal-article","created":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T11:49:26Z","timestamp":1775648966000},"source":"Crossref","is-referenced-by-count":0,"title":["ZeCardioAI: combining zebrafish, AI, and xAI for an in-depth cardiac phenotyping platform"],"prefix":"10.1093","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-4037-3457","authenticated-orcid":false,"given":"Ferran","family":"Arqu\u00e9","sequence":"first","affiliation":[{"name":"Early Drug Discovery Department, ZeCardio Therapeutics, Carrer de Laure\u00e0 Mir\u00f3 , 408, 410, 08980 Barcelona ,","place":["Spain"]},{"name":"Technology and Innovation Department, ZeClinics SL, Carrer de Laure\u00e0 Mir\u00f3 , 408, 410, 08980 Barcelona ,","place":["Spain"]},{"name":"Biomedical Data Science Group, Institut de Salut Global de Barcelona (ISGlobal), Carrer del Doctor Aiguader , 88, 08003 Barcelona ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0239-0343","authenticated-orcid":false,"given":"Carole","family":"Jung","sequence":"additional","affiliation":[{"name":"Technology and Innovation Department, ZeClinics SL, Carrer de Laure\u00e0 Mir\u00f3 , 408, 410, 08980 Barcelona ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6683-9082","authenticated-orcid":false,"given":"Beatriz","family":"Muriel-Moreno","sequence":"additional","affiliation":[{"name":"Technology and Innovation Department, ZeClinics SL, Carrer de Laure\u00e0 Mir\u00f3 , 408, 410, 08980 Barcelona ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8905-7094","authenticated-orcid":false,"given":"Laura","family":"L\u00f3pez-Blanch","sequence":"additional","affiliation":[{"name":"Technology and Innovation Department, ZeClinics SL, Carrer de Laure\u00e0 Mir\u00f3 , 408, 410, 08980 Barcelona ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2825-8172","authenticated-orcid":false,"given":"Eduardo","family":"Saman","sequence":"additional","affiliation":[{"name":"Technology and Innovation Department, ZeClinics SL, Carrer de Laure\u00e0 Mir\u00f3 , 408, 410, 08980 Barcelona ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9000-7403","authenticated-orcid":false,"given":"Davide","family":"D\u2019Amico","sequence":"additional","affiliation":[{"name":"Technology and Innovation Department, ZeClinics SL, Carrer de Laure\u00e0 Mir\u00f3 , 408, 410, 08980 Barcelona ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0426-9862","authenticated-orcid":false,"given":"Javier","family":"Terriente","sequence":"additional","affiliation":[{"name":"Technology and Innovation Department, ZeClinics SL, Carrer de Laure\u00e0 Mir\u00f3 , 408, 410, 08980 Barcelona ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5030-1915","authenticated-orcid":false,"given":"Paula","family":"Petrone","sequence":"additional","affiliation":[{"name":"Biomedical Data Science Group, Institut de Salut Global de Barcelona (ISGlobal), Carrer del Doctor Aiguader , 88, 08003 Barcelona ,","place":["Spain"]},{"name":"Life Sciences - Digital Health Unit, Barcelona Supercomputing Center (BSC-CNS), Pla\u00e7a d\u2019Eusebi G\u00fcell , 1-3, 08034 Barcelona ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2485-0638","authenticated-orcid":false,"given":"Sylvia","family":"Dyballa","sequence":"additional","affiliation":[{"name":"Technology and Innovation Department, ZeClinics SL, Carrer de Laure\u00e0 Mir\u00f3 , 408, 410, 08980 Barcelona ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,4,25]]},"reference":[{"key":"2026081008235670400_ref1","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2022.1042448","article-title":"National, regional, and global cardiomyopathy burden from 1990 to 2019","volume":"9","author":"Cheng","year":"2022","journal-title":"Front Cardiovasc Med"},{"key":"2026081008235670400_ref2","doi-asserted-by":"publisher","first-page":"998","DOI":"10.1016\/S0140-6736(23)01241-2","article-title":"Dilated cardiomyopathy: causes, mechanisms, and current and future treatment 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