{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:05:34Z","timestamp":1784592334151,"version":"3.55.0"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T00:00:00Z","timestamp":1784505600000},"content-version":"vor","delay-in-days":19,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFC2307704"],"award-info":[{"award-number":["2023YFC2307704"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"CAMS Innovation Fund for Medical Sciences","award":["2024-I2M-TS-030I\/2023-I2M-QJ-018"],"award-info":[{"award-number":["2024-I2M-TS-030I\/2023-I2M-QJ-018"]}]},{"name":"Yunnan Revitalization Talent Support Program"},{"name":"Science and Technology Plan-biological medical special project of Yunnan Province","award":["202302AA310005"],"award-info":[{"award-number":["202302AA310005"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Inducing strong and targeted humoral immune responses, a central goal of next-generation vaccine development, involves a complex series of interconnected steps. However, a comprehensive study that addresses and integrates all steps is still missing. Here, we present a study that addresses these steps to elicit precise neutralizing antibody (nAb) responses targeting focused epitope motifs. We present a new protein design model called EpiTopoFold for constructing a topology that precisely accommodates and stabilizes the epitope motif. After ten thousand in silico simulations, we designed a de novo antigen L9, which structurally incorporated three epitope motifs from the receptor-binding domain (RBD) of the coronavirus spike protein. To enhance antigen immunogenicity, we displayed L9 on the surface of yeast (Saccharomyces cerevisiae). In mice, the yeast-displayed L9 induced stronger and broader neutralizing responses than the alum-adjuvanted L9 or commercial RBD protein. Together, this work establishes a proof-of-concept framework that integrates intelligent antigen design with yeast surface display to enable robust and targeted nAb responses for next-generation vaccine development.<\/jats:p>","DOI":"10.1093\/bib\/bbag393","type":"journal-article","created":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T23:18:33Z","timestamp":1784589513000},"source":"Crossref","is-referenced-by-count":0,"title":["Intelligent antigen design combined with yeast display enhances targeted immune responses"],"prefix":"10.1093","volume":"27","author":[{"given":"Yun-Fei","family":"Ma","sequence":"first","affiliation":[{"name":"Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College , No. 935, Jiaoling Road, Kunming, Yunnan Province 650118 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guang-Hui","family":"Yin","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College , No. 935, Jiaoling Road, Kunming, Yunnan Province 650118 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Pan","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College , No. 935, Jiaoling Road, Kunming, Yunnan Province 650118 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5581-9046","authenticated-orcid":false,"given":"Ye","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College , No. 935, Jiaoling Road, Kunming, Yunnan Province 650118 ,","place":["China"]},{"name":"State Key Laboratory of Respiratory Health and Multimorbidity , No. 9 Dongdan San Tiao, Dongcheng District, Beijing 100190 ,","place":["China"]},{"name":"Key Laboratory of Pathogen Infection Prevention and Control (Peking Union Medical College), Ministry of Education , No. 9 Dongdan San Tiao, Dongcheng District, Beijing 100190 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,7,20]]},"reference":[{"key":"2026072019182759300_ref1","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1016\/j.tim.2017.09.009","article-title":"Clinical potential of prefusion RSV F-specific antibodies","volume":"26","author":"Rossey","year":"2018","journal-title":"Trends Microbiol"},{"key":"2026072019182759300_ref2","doi-asserted-by":"publisher","first-page":"2447615","DOI":"10.1080\/22221751.2024.2447615","article-title":"Enhanced antibody response to the conformational non-RBD region via DNA prime-protein boost elicits broad cross-neutralization against SARS-CoV-2 variants","volume":"14","author":"Ma","year":"2025","journal-title":"Emerg 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