{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T00:04:59Z","timestamp":1765670699256,"version":"3.48.0"},"reference-count":9,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100021073","name":"Rosalind Franklin Institute","doi-asserted-by":"publisher","award":["BB\/V018523\/1"],"award-info":[{"award-number":["BB\/V018523\/1"]}],"id":[{"id":"10.13039\/100021073","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Motivation<\/jats:title>\n                    <jats:p>Nanobodies are single domain antibodies derived from the unique heavy chain-only immunoglobulins of the camelid family, and have broad applications in biosciences, including as imaging and diagnostic agents. The generation of nanobodies is a multi-stage experimental process for which we have developed AntigenApp a laboratory data management system for nanobody generation and sequence analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>AntigenApp is a web application and database built to capture data generated during all steps of the nanobody discovery pipeline and to provide built-in analysis capabilities of the experimental results. This includes enzyme-linked immunosorbent assay optical density measurements, sequencing results, nanobody domain labelling, and search\/clustering capabilities using protein BLAST. The tool incorporates a standardized naming convention that is automatically applied to nanobodies stored in the database and we envisage the tool being of interest to the wider nanobody community for in-house use.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Availability and implementation<\/jats:title>\n                    <jats:p>AntigenApp is available from https:\/\/github.com\/rosalindfranklininstitute\/antigen-app under the Apache 2.0 licence and published https:\/\/doi.org\/10.5281\/zenodo.17397055.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btaf642","type":"journal-article","created":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T13:13:06Z","timestamp":1764335586000},"source":"Crossref","is-referenced-by-count":0,"title":["AntigenApp: a laboratory data management system for nanobody generation and sequence analysis"],"prefix":"10.1093","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6950-8908","authenticated-orcid":false,"given":"Alexander L R","family":"Lubbock","sequence":"first","affiliation":[{"name":"The Rosalind Franklin Institute, Harwell Science Campus , Didcot, OX11 0QX,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lauren E -A","family":"Eyssen","sequence":"additional","affiliation":[{"name":"The Rosalind Franklin Institute, Harwell Science Campus , Didcot, OX11 0QX,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelly","family":"Parker","sequence":"additional","affiliation":[{"name":"The Rosalind Franklin Institute, Harwell Science Campus , Didcot, OX11 0QX,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Basham","sequence":"additional","affiliation":[{"name":"The Rosalind Franklin Institute, Harwell Science 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