{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T01:38:26Z","timestamp":1792201106354,"version":"4.4.0"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2020,3,13]],"date-time":"2020-03-13T00:00:00Z","timestamp":1584057600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100011773","name":"Regional Council of Burgundy","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011773","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,5,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>At labor, the myometrium is infiltrated by a massive influx of macrophages that secrete high levels of pro-inflammatory cytokines inducing the expression of specific labor-associated markers. However, the interactions between myocytes and macrophages and the role of macrophages in the myometrium at labor remain to be elucidated. In this work, we studied the role of myometrium-infiltrated macrophages and their interaction with myocytes in lipopolysaccharide-induced preterm labor. A co-culture model of human primary myometrial cells and macrophages was developed and validated. Collagen lattices were used to evaluate myocyte contraction. Differentiation steps were assessed by (i) phalloidin and vinculin staining for cytoskeleton reorganization, (ii) gap junction protein alpha 1 expression and scrape loading\/dye transfer with Lucifer Yellow for gap junction intercellular communication, and (iii) calcium imaging for cell excitability. We demonstrated that macrophages favored lipopolysaccharide-induced contraction and early differentiation of myometrial cells. Transwell assays showed that previous activation of macrophages by lipopolysaccharide was essential for this differentiation and that macrophage\/myocyte interactions involved macrophage release of reactive oxygen species (ROS). The effects of macrophage-released ROS in myometrial cell transactivation were mimicked by H2O2, suggesting that superoxide anion is a major intermediate messenger in macrophage\/myocyte crosstalk during labor. These novel findings provide the foundation for innovative approaches to managing preterm labor, specifically the use of antioxidants to inhibit the initial stages of labor before the contractile phenotype has been acquired. In addition, the co-culture model developed by our team could be used in future research to decipher pathophysiological signaling pathways or screen\/develop new tocolytics.<\/jats:p>","DOI":"10.1093\/biolre\/ioaa032","type":"journal-article","created":{"date-parts":[[2020,3,13]],"date-time":"2020-03-13T09:27:32Z","timestamp":1584091652000},"page":"1326-1339","source":"Crossref","is-referenced-by-count":26,"title":["Macrophage-induced reactive oxygen species promote myometrial contraction and labor-associated mechanisms\u2020"],"prefix":"10.1093","volume":"102","author":[{"given":"Maeva","family":"Wendremaire","sequence":"first","affiliation":[{"name":"Institut National de la Sant\u00e9c et de la Recherche M\u00e9dicale, Lipides Nutrition Cancer, Dijon, France"},{"name":"Universit\u00e9 de Bourgogne Franche-Comt\u00e9, Lipides Nutrition Cancer, Dijon, France"},{"name":"Laboratoire de Pharmacologie-Toxicologie, CHU Dijon-Bourgogne, Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarik","family":"Hadi","sequence":"first","affiliation":[{"name":"Institut National de la Sant\u00e9c et de la Recherche M\u00e9dicale, Lipides Nutrition Cancer, Dijon, France"},{"name":"Universit\u00e9 de Bourgogne Franche-Comt\u00e9, Lipides Nutrition Cancer, Dijon, France"},{"name":"Department of Cardiac Surgery, NYU Langone Medical Center, New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Pezze","sequence":"first","affiliation":[{"name":"Institut National de la Sant\u00e9c et de la Recherche M\u00e9dicale, Lipides Nutrition Cancer, Dijon, France"},{"name":"Universit\u00e9 de Bourgogne Franche-Comt\u00e9, Lipides Nutrition Cancer, Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Barrichon","sequence":"first","affiliation":[{"name":"Institut National de la Sant\u00e9c et de la Recherche M\u00e9dicale, Lipides Nutrition Cancer, Dijon, France"},{"name":"Universit\u00e9 de Bourgogne Franche-Comt\u00e9, Lipides Nutrition Cancer, Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana","family":"Lopez","sequence":"first","affiliation":[{"name":"Institut National de la Sant\u00e9c et de la Recherche M\u00e9dicale, Lipides Nutrition Cancer, Dijon, France"},{"name":"Universit\u00e9 de Bourgogne Franche-Comt\u00e9, Lipides Nutrition Cancer, Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrice","family":"Neiers","sequence":"first","affiliation":[{"name":"Centre des Sciences du Go\u00fbt et de l'Alimentation, INRA, CNRS, Universit\u00e9 de Bourgogne Franche-Comt\u00e9, Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Sagot","sequence":"first","affiliation":[{"name":"Service de Gyn\u00e9cologie-Obst\u00e9trique, CHU Dijon-Bourgogne, Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Garrido","sequence":"first","affiliation":[{"name":"Institut National de la Sant\u00e9c et de la Recherche M\u00e9dicale, Lipides Nutrition Cancer, Dijon, France"},{"name":"Universit\u00e9 de Bourgogne Franche-Comt\u00e9, Lipides Nutrition Cancer, Dijon, France"},{"name":"Centre Georges Fran\u00e7ois Leclerc, Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Lirussi","sequence":"first","affiliation":[{"name":"Institut National de la Sant\u00e9c et de la Recherche M\u00e9dicale, Lipides Nutrition Cancer, Dijon, France"},{"name":"Universit\u00e9 de Bourgogne Franche-Comt\u00e9, Lipides Nutrition Cancer, Dijon, France"},{"name":"Laboratoire de Pharmacologie-Toxicologie, CHU Dijon-Bourgogne, Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,3,13]]},"reference":[{"key":"2020052802500120400_ref1","doi-asserted-by":"crossref","first-page":"S2","DOI":"10.1186\/1742-4755-10-S1-S2","article-title":"Born too soon preterm birth action group. 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