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Upon ejaculation, they must immediately start producing sufficient energy to maintain motility and support capacitation. While this increased energy demand during capacitation is well established, it remains unclear how mouse sperm modify their metabolism to meet this need. We now show that capacitating mouse sperm enhance glucose uptake, identifying glucose uptake as a functional marker of capacitation. Using an extracellular flux analyzer, we show that glycolysis and oxidative phosphorylation increase during capacitation. Furthermore, this increase in oxidative phosphorylation is dependent on glycolysis, providing experimental evidence for a link between glycolysis and oxidative phosphorylation in mouse sperm.<\/jats:p>","DOI":"10.1093\/biolre\/ioaa114","type":"journal-article","created":{"date-parts":[[2020,7,2]],"date-time":"2020-07-02T10:44:03Z","timestamp":1593686643000},"page":"791-801","source":"Crossref","is-referenced-by-count":90,"title":["Metabolic changes in mouse sperm during capacitation\u2020"],"prefix":"10.1093","volume":"103","author":[{"given":"Melanie","family":"Balbach","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Weill Cornell Medical College, New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Gracia","family":"Gervasi","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, Integrated Science Building (ISB), University of Massachusetts, Amherst, MA, 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