{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T08:26:57Z","timestamp":1767860817159,"version":"3.49.0"},"reference-count":56,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2020,8,6]],"date-time":"2020-08-06T00:00:00Z","timestamp":1596672000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"National Transgenic Major Program of China","award":["2016ZX08006003-006"],"award-info":[{"award-number":["2016ZX08006003-006"]}]},{"name":"Key-Area Research and Development Program of Guangdong","award":["2018B020203003"],"award-info":[{"award-number":["2018B020203003"]}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2019A1515011134"],"award-info":[{"award-number":["2019A1515011134"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,10,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Bone morphogenetic protein 15 (BMP15), a member of the transforming growth factor beta superfamily, plays an essential role in ovarian follicular development in mono-ovulatory mammalian species. Studies using a biallelic knockout mouse model revealed that BMP15 potentially has just a minimal impact on female fertility and ovarian follicular development in polyovulatory species. In contrast, our previous study demonstrated that in vivo knockdown of BMP15 significantly affected porcine female fertility, as evidenced by the dysplastic ovaries containing significantly decreased numbers of follicles and an increased number of abnormal follicles. This finding implied that BMP15 plays an important role in the regulation of female fertility and ovarian follicular development in polyovulatory species. To further investigate the regulatory role of BMP15 in porcine ovarian and follicular development, here, we describe the efficient generation of BMP15-edited Yorkshire pigs using CRISPR\/Cas9. Using artificial insemination experiments, we found that the biallelically edited gilts were all infertile, regardless of different genotypes. One monoallelically edited gilt #4 (\u039466 bp\/WT) was fertile and could deliver offspring with a litter size comparable to that of wild-type gilts. Further analysis established that the infertility of biallelically edited gilts was caused by the arrest of follicular development at preantral stages, with formation of numerous structurally abnormal follicles, resulting in streaky ovaries and the absence of obvious estrous cycles. Our results strongly suggest that the role of BMP15 in nonrodent polyovulatory species may be as important as that in mono-ovulatory species.<\/jats:p>","DOI":"10.1093\/biolre\/ioaa138","type":"journal-article","created":{"date-parts":[[2020,8,6]],"date-time":"2020-08-06T18:53:25Z","timestamp":1596740005000},"page":"1054-1068","source":"Crossref","is-referenced-by-count":8,"title":["Efficient generation of bone morphogenetic protein 15-edited Yorkshire pigs using CRISPR\/Cas9\u2020"],"prefix":"10.1093","volume":"103","author":[{"given":"Xuan","family":"Shi","sequence":"first","affiliation":[{"name":"State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Tang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiyuan","family":"Lin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongbo","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yufeng","family":"Qin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinyu","family":"Liang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, P.R. 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