{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:14:49Z","timestamp":1787008489205,"version":"build-2736575974"},"reference-count":61,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2021,2,8]],"date-time":"2021-02-08T00:00:00Z","timestamp":1612742400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Cecilia Valencia\u2019s Doctoral Fellowship"},{"name":"Directorate of Research of Universidad de La Frontera"},{"name":"ANID\/CONICYT Chile","award":["FONDECYT 1181453"],"award-info":[{"award-number":["FONDECYT 1181453"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,5,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The present study evaluated the mechanism by which protein synthesis inhibitors activate bovine oocytes. The aim was to analyze the dynamics of MPF and MAPKs. MII oocytes were activated with ionomycin (Io), ionomycin+anisomycin (ANY) and ionomycin+cycloheximide (CHX) and by in vitro fertilization (IVF). The expression of cyclin B1, p-CDK1, p-ERK1\/2, p-JNK, and p-P38 were evaluated by immunodetection and the kinase activity of ERK1\/2 was measured by enzyme assay. Evaluations at 1, 4, and 15\u00a0hours postactivation (hpa) showed that the expression of cyclin B1 was not modified by the treatments. ANY inactivated MPF by p-CDK1Thr14-Tyr15 at 4\u00a0hpa (P\u00a0&amp;lt;\u00a00.05), CHX increased pre-MPF (p-CDK1Thr161 and p-CDK1Thr14-Tyr15) at 1\u00a0hpa and IVF increased p-CDK1Thr14-Tyr15 at 17\u00a0hours postfertilization (hpf) (P\u00a0&amp;lt;\u00a00.05). ANY and CHX reduced the levels of p-ERK1\/2 at 4\u00a0hpa (P\u00a0&amp;lt;\u00a00.05) and its activity at 4 and 1\u00a0hpa, respectively (P\u00a0&amp;lt;\u00a00.05). Meanwhile, IVF increased p-ERK1\/2 at 6\u00a0hpf (P\u00a0&amp;lt;\u00a00.05); however, its kinase activity decreased at 6\u00a0hpf (P\u00a0&amp;lt;\u00a00.05). p-JNK in ANY, CHX, and IVF oocytes decreased at 4\u00a0hpa (P\u00a0&amp;lt;\u00a00.05). p-P38 was only observed at 1\u00a0hpa, with no differences between treatments. In conclusion, activation of bovine oocytes by ANY, CHX, and IVF inactivates MPF by CDK1-dependent specific phosphorylation without cyclin B1 degradation. ANY or CHX promoted this inactivation, which seemed to be more delayed in the physiological activation (IVF). Both inhibitors modulated MPF activity via an ERK1\/2-independent pathway, whereas IVF activated the bovine oocytes via an ERK1\/2-dependent pathway. Finally, ANY does not activate the JNK and P38 kinase pathways.<\/jats:p>","DOI":"10.1093\/biolre\/ioab019","type":"journal-article","created":{"date-parts":[[2021,2,7]],"date-time":"2021-02-07T14:07:46Z","timestamp":1612706866000},"page":"1126-1138","source":"Crossref","is-referenced-by-count":13,"title":["Activation of bovine oocytes by protein synthesis inhibitors: new findings on the role of MPF\/MAPKs\u2020"],"prefix":"10.1093","volume":"104","author":[{"given":"Cecilia","family":"Valencia","sequence":"first","affiliation":[{"name":"Laboratory of Reproduction, Centre of Reproductive Biotechnology (CEBIOR-BIOREN), Universidad de La Frontera, Temuco, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe Alonso","family":"P\u00e9rez","sequence":"additional","affiliation":[{"name":"Laboratory of Reproduction, Centre of Reproductive Biotechnology (CEBIOR-BIOREN), Universidad de La Frontera, Temuco, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carola","family":"Matus","sequence":"additional","affiliation":[{"name":"Center of Molecular Biology and Pharmacogenetics, Department of Basic Sciences, Faculty of Medicine, Universidad de La Frontera, Temuco, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo","family":"Felmer","sequence":"additional","affiliation":[{"name":"Laboratory of Reproduction, Centre of Reproductive Biotechnology (CEBIOR-BIOREN), Universidad de La Frontera, Temuco, Chile"},{"name":"Department of Agricultural Sciences and Natural Resources, Faculty of Agriculture and Forestry, Universidad de La Frontera, Temuco, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Elena","family":"Arias","sequence":"additional","affiliation":[{"name":"Laboratory of Reproduction, Centre of Reproductive Biotechnology (CEBIOR-BIOREN), Universidad de La Frontera, Temuco, Chile"},{"name":"Department of 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