{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T09:46:21Z","timestamp":1751363181807,"version":"3.37.3"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2021,3,2]],"date-time":"2021-03-02T00:00:00Z","timestamp":1614643200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81471455","81100418"],"award-info":[{"award-number":["81471455","81100418"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,6,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The objective is to investigate the pathophysiological significance of Par3 and integrin \u03b21 with regard to the functionality of the endometrial luminal epithelium (LE). Design: laboratory study; setting: university research laboratory. Analysis involved endometrial aspirates and endometrial adenocarcinoma cells (HEC-1A) and endometrial carcinoma cells (RL95-2). We first examined the expression and localization of Par3 and integrin \u03b21 in HEC-1A cells and RL95-2 cells. Then we knocked down Par3 and integrin \u03b21 in HEC-1A cells and RL95-2 cells, respectively, and found that Par3\/integrin \u03b21 affected embryo adhesion by regulating the intercellular tight junctions\u2019 (TJs\u2019) structure and thus the polarity of the endometrial LE. These findings were also confirmed in the endometrium specimens from human and mice. The main outcome measures were the expression and localization of Par3 and integrin \u03b21 in the endometrial epithelial cell lines and endometrium specimens and the regulations of Par3 and integrin \u03b21 on TJs, polarity, and embryo adhesion. Following the knockdown of Par3 in HEC-1A cells, there was a reduction in the complexity of the TJs and cell polarity, and the adhered blastocysts number was significantly increased. However, the reduction of integrin \u03b21 in RL95-2 cells resulted in effects that directly opposed those following the knockdown of Par3 in HEC-1A cells. Estrogen and progesterone reduced the expression of Par3 and promoted the expression of integrin \u03b21 in HEC-1A cells. Par3\/integrin \u03b21 regulates embryo adhesion by regulating intercellular TJs\u2019 structure and polarity of endometrial LE under the action of ovarian hormones.<\/jats:p>","DOI":"10.1093\/biolre\/ioab033","type":"journal-article","created":{"date-parts":[[2021,3,6]],"date-time":"2021-03-06T20:03:00Z","timestamp":1615060980000},"page":"1228-1238","source":"Crossref","is-referenced-by-count":4,"title":["Par3\/integrin <b>\u03b2<\/b>1 regulates embryo adhesion via changing endometrial luminal epithelium polarity"],"prefix":"10.1093","volume":"104","author":[{"given":"Jiali","family":"Peng","sequence":"first","affiliation":[{"name":"Center of Reproductive Medicine, Renmin Hospital of Wuhan University, Wuhan, Hubei, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoling","family":"Li","sequence":"additional","affiliation":[{"name":"Center of Reproductive Medicine, Renmin Hospital of Wuhan University, Wuhan, Hubei, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Center of Reproductive Medicine, Renmin Hospital of Wuhan University, Wuhan, Hubei, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Hu","sequence":"additional","affiliation":[{"name":"Center of Reproductive Medicine, Renmin Hospital of Wuhan University, Wuhan, Hubei, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunjie","family":"Shang","sequence":"additional","affiliation":[{"name":"Center of Reproductive Medicine, Renmin Hospital of Wuhan University, Wuhan, Hubei, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuchen","family":"Yin","sequence":"additional","affiliation":[{"name":"Center of Reproductive Medicine, Renmin Hospital of Wuhan University, Wuhan, Hubei, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuoni","family":"Xiao","sequence":"additional","affiliation":[{"name":"Center of Reproductive Medicine, Renmin Hospital of Wuhan University, Wuhan, Hubei, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,3,2]]},"reference":[{"key":"2021060706280209500_ref1","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1038\/sj.cr.7290227","article-title":"Uterine receptivity and the plasma membrane transformation","volume":"14","author":"Murphy","year":"2004","journal-title":"Cell Res"},{"key":"2021060706280209500_ref2","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1210\/en.2016-1877","article-title":"The endometrial polarity paradox: differential regulation of polarity within secretory-phase human 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