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Such defects may also involve the population of small non-coding RNAs (sncRNAs), which are important during early embryonic development. The objective of this study was to examine dynamic changes in relative abundance of sncRNAs during the maternal-to-embryonic transition (MET) in bovine embryos produced by scNT as compared to IVF by using RNA sequencing. When comparing populations of miRNA in scNT versus IVF embryos, only miR-2340, miR-345, and miR34a were differentially expressed in morulae, though many more miRNAs were differentially expressed when comparing across developmental stages. Also of interest, distinct populations of piwi-interacting like RNAs (pilRNAs) were identified in bovine embryos prior to and during embryonic genome activation (EGA) as compared bovine embryos post-EGA and differentiated cells. Overall, sncRNA sequencing analysis of preimplantation embryos revealed largely similar profiles of sncRNAs for IVF and scNT embryos at the 2-cell, 8-cell, morula, and blastocyst stages of development. However, these sncRNA profiles, including miRNA, piRNA, and tRNA fragments, were notably distinct prior to and after completion of the MET.<\/jats:p>","DOI":"10.1093\/biolre\/ioab107","type":"journal-article","created":{"date-parts":[[2021,6,4]],"date-time":"2021-06-04T15:56:38Z","timestamp":1622822198000},"page":"918-933","source":"Crossref","is-referenced-by-count":14,"title":["Dynamics of small non-coding RNAs in bovine scNT embryos through the maternal-to-embryonic transition"],"prefix":"10.1093","volume":"105","author":[{"given":"Jocelyn M","family":"Cuthbert","sequence":"first","affiliation":[{"name":"Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stewart J","family":"Russell","sequence":"additional","affiliation":[{"name":"CReATe Fertility Centre, Toronto, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina A","family":"Polejaeva","sequence":"additional","affiliation":[{"name":"Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinggang","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth L","family":"White","sequence":"additional","affiliation":[{"name":"Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abby D","family":"Benninghoff","sequence":"additional","affiliation":[{"name":"Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT, 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