{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T05:49:13Z","timestamp":1790401753279,"version":"4.1.0"},"reference-count":88,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2021,6,8]],"date-time":"2021-06-08T00:00:00Z","timestamp":1623110400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100000024","name":"CIHR","doi-asserted-by":"publisher","award":["84576"],"award-info":[{"award-number":["84576"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Canada Research Chair in Reproductive Toxicology"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,10,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The epididymis is composed of a pseudostratified epithelium that is comprised of various cell types. Studies have shown that rat basal cells share common properties with adult stem cells and begin to differentiate in vitro in response to fibroblast growth factor and 5\u03b1-dihydrotestosterone. The characterization of rat basal cells is therefore necessary to fully understand the role of these cells. The objectives of this study were to assess the ability of single basal cells to develop organoids and to assess their ability to self-renew and differentiate in vitro. We isolated basal cells from the rat epididymis and established three-dimensional cell cultures from the basal and nonbasal cell fractions. Organoids were formed by single adult epididymal basal cells. Organoids were dissociated into single basal cells, which were able to reform new organoids, and were maintained over 10 generations. Long-term culture of organoids revealed that these cells could be differentiated into cells expressing the principal cell markers aquaporin 9 and cystic fibrosis transmembrane conductance regulator. Electron microscopy demonstrated that organoids were composed of several polarized cell types displaying microvilli and the ability to form tight junctions. Additionally, organoids could be formed by basal cells from either the proximal or distal region of the epididymis and are able to secrete clusterin, a protein implicated in the maturation of spermatozoa. These data indicate that rat basal cells can be used to derive epididymal organoids and further support that notion that these may represent a stem cell population in the epididymis.<\/jats:p>","DOI":"10.1093\/biolre\/ioab113","type":"journal-article","created":{"date-parts":[[2021,6,9]],"date-time":"2021-06-09T01:58:16Z","timestamp":1623203896000},"page":"987-1001","source":"Crossref","is-referenced-by-count":26,"title":["Self-renewal and differentiation of rat epididymal basal cells using a novel in vitro organoid model"],"prefix":"10.1093","volume":"105","author":[{"given":"Laurie","family":"Pinel","sequence":"first","affiliation":[{"name":"Laboratory for Reproductive Toxicology, INRS-Centre Armand-Frappier Sant\u00e9 Biotechnologie, University of Quebec, Laval, QC, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel G","family":"Cyr","sequence":"additional","affiliation":[{"name":"Laboratory for Reproductive Toxicology, INRS-Centre Armand-Frappier Sant\u00e9 Biotechnologie, University of Quebec, Laval, QC, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,6,8]]},"reference":[{"key":"2021101306332666300_ref1","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1051\/rnd:19670403","article-title":"Maturation of spermatozoa in the rabbit epididymis: fertilizing ability and embryonic mortality in does inseminated with epididymal spermatozoa","volume":"7","author":"Orgebin-Crist","year":"1967","journal-title":"Ann Biol Anim Biochim Biophys"},{"key":"2021101306332666300_ref2","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1007\/978-1-4615-0679-9_21","volume-title":"The Epididymis: From Molecules to Cinical Practice","author":"Olson","year":"2002"},{"key":"2021101306332666300_ref3","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.theriogenology.2004.09.015","article-title":"Epididymal cell secretory activities and the role of proteins 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