{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T17:56:11Z","timestamp":1783619771228,"version":"3.55.0"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2021,8,5]],"date-time":"2021-08-05T00:00:00Z","timestamp":1628121600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>In cattle, uterine luminal fluid (ULF) is the main source of molecules that support embryo development and survival during the peri-implantation period. Our overarching hypothesis is that peri-estrus changes in uterine function, including ULF accumulation and absorption, are uneven among individuals, and affect ULF composition and fertility. Our objectives were (1) to characterize temporal and spatial changes in ULF volume, endometrial and luteal blood perfusion, endometrial and luteal size, and circulating progesterone concentrations during the peri-estrus period in beef heifers and (2) to associate such changes with the metabolite composition in the ULF, 4 days after estrus (d 0). Fourteen Bos indicus heifer that presented a PGF2\u03b1 responsive CL received 500\u00a0\u03bcg PGF2\u03b1 analog i.m. and were examined daily by rectal B-mode and pulse-wave color-Doppler ultrasonography until the fifth day after estrus (d 5). The composition of the ULF was analyzed by targeted mass spectrometry on d 4. Multivariate analyses clustered heifers according to ovarian, uterine, and hormonal variables in clusters A (n\u2009=\u20095) and B (n\u2009=\u20098 heifers). Concentrations of Pro, Ala, Leu, Gly, Val, Lys, Ile, Phe, Asp, Orn, Tyr, Arg, Trp, Suc, Cit, ADMA, the sum of essential Amino Acids (AA), sum of nonessential AA, sum of aromatic AA, and total AA were greater in cluster A (FDR\u2009\u2264\u20090.05). ULF volume dynamics and uterine, ovarian, and hormonal variables during the peri-estrus period presented a concerted variation among heifers within clusters, which was associated with the ULF composition 4\u00a0days after estrus.<\/jats:p>","DOI":"10.1093\/biolre\/ioab149","type":"journal-article","created":{"date-parts":[[2021,8,5]],"date-time":"2021-08-05T12:12:25Z","timestamp":1628165545000},"page":"1140-1153","source":"Crossref","is-referenced-by-count":18,"title":["Peri-estrus ovarian, uterine, and hormonal variables determine the uterine luminal fluid metabolome in beef heifers"],"prefix":"10.1093","volume":"105","author":[{"given":"Felipe A C C","family":"Silva","sequence":"first","affiliation":[{"name":"Department of Animal Sciences, University of Florida, Gainesville, FL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela F","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction, School of Veterinary Medicine and Animal Sciences, University of S\u00e3o Paulo, Pirassununga, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruna S","family":"Vieira","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction, School of Veterinary Medicine and Animal Sciences, University of S\u00e3o Paulo, Pirassununga, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adomar L","family":"Neto","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction, School of Veterinary Medicine and Animal Sciences, University of S\u00e3o Paulo, Pirassununga, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia C","family":"Rocha","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, University of Florida, Gainesville, FL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edson G","family":"Lo Turco","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Urology, Federal University of S\u00e3o Paulo, S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guilherme P","family":"Nogueira","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, S\u00e3o Paulo State University, Ara\u00e7atuba, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guilherme","family":"Pugliesi","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction, School of Veterinary Medicine and Animal Sciences, University of S\u00e3o Paulo, Pirassununga, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Binelli","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, University of Florida, Gainesville, FL, 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