{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T17:19:39Z","timestamp":1788196779598,"version":"build-2803163510"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2021,11,2]],"date-time":"2021-11-02T00:00:00Z","timestamp":1635811200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20190081"],"award-info":[{"award-number":["BK20190081"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31771654"],"award-info":[{"award-number":["31771654"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81701446"],"award-info":[{"award-number":["81701446"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2016YFA0500902"],"award-info":[{"award-number":["2016YFA0500902"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,1,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Infertility affects 10\u201315% of families worldwide. However, the pathogenesis of female infertility caused by abnormal early embryonic development is not clear. A recent study showed that poly(A)binding protein nuclear 1-like (PABPN1L) recruited BTG anti-proliferation factor 4 (BTG4) to mRNA 3\u2032-poly(A) tails and was essential for maternal mRNA degradation. Here, we generated a PABPN1L-antibody and found \u201cring-like\u201d PABPN1L aggregates in the cytoplasm of MII oocytes. PABPN1L\u2013EGFP proteins spontaneously formed \u201cring-like\u201d aggregates in vitro. This phenomenon is similar with CCR4\u2013NOT catalytic subunit, CCR4-NOT transcription complex subunit 7 (CNOT7), when it starts deadenylation process in vitro. We constructed two mouse model (Pabpn1l\u2212\/\u2212 and Pabpn1l \u00a0tm1a\/tm1a) simulating the intron 1\u2013exon 2 abnormality of human PABPN1L and found that the female was sterile and the male was fertile. Using RNA-Seq, we observed a large-scale up-regulation of RNA in zygotes derived from Pabpn1l\u2212\/\u2212 MII oocytes. We found that 9222 genes were up-regulated instead of being degraded in the Pabpn1l\u2212\u2640\/+\u2642zygote. Both the Btg4 and CCR4-NOT transcription complex subunit 6 like (Cnot6l) genes are necessary for the deadenylation process and Pabpn1l\u2212\/\u2212 resembled both the Btg4 and Cnot6l knockouts, where 71.2% genes stabilized in the Btg4\u2212\u2640\/+\u2642 zygote and 84.2% genes stabilized in the Cnot6l\u2212\u2640\/+\u2642zygote were also stabilized in Pabpn1l\u2212\u2640\/+\u2642 zygote. BTG4\/CNOT7\/CNOT6L was partially co-located with PABPN1L in MII oocytes. The above results suggest that PABPN1L is widely associated with CCR4\u2013NOT-mediated maternal mRNA degradation and PABPN1L variants on intron 1\u2013exon 2 could be a genetic marker of female infertility.<\/jats:p>","DOI":"10.1093\/biolre\/ioab203","type":"journal-article","created":{"date-parts":[[2021,11,2]],"date-time":"2021-11-02T11:55:57Z","timestamp":1635854157000},"page":"83-94","source":"Crossref","is-referenced-by-count":2,"title":["PABPN1L assemble into \u201cring-like\u201d aggregates in the cytoplasm of MII oocytes and is associated with female infertility\u2020"],"prefix":"10.1093","volume":"106","author":[{"given":"Ying","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianhao","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingcong","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaodan","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zerui","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jintao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuqin","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junqiang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiufeng","family":"Ling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxi","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,11,2]]},"reference":[{"key":"2022011317375119500_ref1","doi-asserted-by":"crossref","first-page":"e1005601","DOI":"10.1371\/journal.pgen.1005601","article-title":"Evidence 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