{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T08:14:11Z","timestamp":1780733651899,"version":"3.54.1"},"reference-count":96,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2022,4,2]],"date-time":"2022-04-02T00:00:00Z","timestamp":1648857600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81971439"],"award-info":[{"award-number":["81971439"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Key R&D Program","award":["2021YFC2700200"],"award-info":[{"award-number":["2021YFC2700200"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,7,25]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Testis, the only organ responsible for generating sperm, is by far the organ with the largest variety of proteins and tissue-specific proteins in humans. In testis, spermatogenesis is a multi-step complex process well-accepted that protein and mRNA are decoupled in certain stages of spermatogenesis. With the fast development of mass spectrometry-based proteomics, it is possible to systemically study protein abundances and modifications in testis and sperm to help us understand the molecular mechanisms of spermatogenesis. This review provides an overview of the recent progress of proteomics analysis on spermatogenesis, including protein expression and multiple post-translational modifications, such as phosphorylation, glycosylation, ubiquitylation, and acetylation.<\/jats:p>","DOI":"10.1093\/biolre\/ioac065","type":"journal-article","created":{"date-parts":[[2022,4,3]],"date-time":"2022-04-03T01:31:03Z","timestamp":1648949463000},"page":"109-117","source":"Crossref","is-referenced-by-count":16,"title":["Recent progress of proteomic analysis on spermatogenesis"],"prefix":"10.1093","volume":"107","author":[{"given":"Haotian","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine , Department of Histology and Embryology, Nanjing Medical University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenghao","family":"Situ","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine , Department of Histology and Embryology, Nanjing Medical University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuejiang","family":"Guo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine , Department of Histology and Embryology, Nanjing Medical University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,4,2]]},"reference":[{"key":"2022072511584691100_ref1","doi-asserted-by":"crossref","first-page":"1651","DOI":"10.1002\/prca.200780120","article-title":"Analysis of the human testis proteome by mass spectrometry and bioinformatics","volume":"2","author":"Guo","year":"2008","journal-title":"Proteomics Clin Appl"},{"key":"2022072511584691100_ref2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1152\/physrev.00013.2015","article-title":"Spermatogenesis: the commitment to meiosis","volume":"96","author":"Griswold","year":"2016","journal-title":"Physiol Rev"},{"key":"2022072511584691100_ref3","doi-asserted-by":"crossref","first-page":"12451","DOI":"10.1073\/pnas.92.26.12451","article-title":"Premature translation of protamine 1 mRNA causes precocious nuclear condensation and arrests spermatid differentiation in mice","volume":"92","author":"Lee","year":"1995","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2022072511584691100_ref4","doi-asserted-by":"crossref","first-page":"2176","DOI":"10.1126\/science.1070963","article-title":"Unique chromatin remodeling and transcriptional regulation in spermatogenesis","volume":"296","author":"Sassone-Corsi","year":"2002","journal-title":"Science"},{"key":"2022072511584691100_ref5","doi-asserted-by":"crossref","first-page":"1566","DOI":"10.1016\/j.cell.2019.11.022","article-title":"A translation-activating function of MIWI\/piRNA during mouse spermiogenesis","volume":"179","author":"Dai","year":"2019","journal-title":"Cell"},{"key":"2022072511584691100_ref6","doi-asserted-by":"crossref","first-page":"2309","DOI":"10.1093\/humrep\/dem115","article-title":"The relationship between perceived stigma, disclosure patterns, support and distress in new attendees at an infertility clinic","volume":"22","author":"Slade","year":"2007","journal-title":"Hum Reprod"},{"key":"2022072511584691100_ref7","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1093\/humupd\/dmv016","article-title":"Infertility around the globe: new thinking on gender, reproductive technologies and global movements in the 21st century","volume":"21","author":"Inhorn","year":"2015","journal-title":"Hum Reprod Update"},{"key":"2022072511584691100_ref8","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/S0140-6736(20)32667-2","article-title":"Male infertility","volume":"397","author":"Agarwal","year":"2021","journal-title":"Lancet"},{"key":"2022072511584691100_ref9","doi-asserted-by":"crossref","first-page":"1786","DOI":"10.1093\/humrep\/dex234","article-title":"The International Glossary on Infertility and Fertility Care, 2017","volume":"32","author":"Zegers-Hochschild","year":"2017","journal-title":"Hum Reprod"},{"key":"2022072511584691100_ref10","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.rbmo.2017.12.005","article-title":"A search for molecular mechanisms underlying male idiopathic infertility","volume":"36","author":"Bracke","year":"2018","journal-title":"Reprod Biomed Online"},{"key":"2022072511584691100_ref11","doi-asserted-by":"crossref","first-page":"5301","DOI":"10.1038\/s41467-020-19045-9","article-title":"A high-stringency blueprint of the human proteome","volume":"11","author":"Adhikari","year":"2020","journal-title":"Nat Commun"},{"key":"2022072511584691100_ref12","doi-asserted-by":"crossref","DOI":"10.1007\/s12038-020-00099-2","article-title":"Evaluation of post-translational modifications in histone proteins: a review on histone modification defects in developmental and neurological disorders","volume":"45","author":"Ramazi","year":"2020","journal-title":"J Biosci"},{"key":"2022072511584691100_ref13","doi-asserted-by":"crossref","first-page":"92","DOI":"10.2174\/1389203721666200726223336","article-title":"Mass spectrometric (MS) analysis of proteins and peptides","volume":"22","author":"Jayathirtha","year":"2021","journal-title":"Curr Protein Pept Sci"},{"key":"2022072511584691100_ref14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-3-319-06068-2_1","article-title":"Mass spectrometry for proteomics-based investigation","volume":"806","author":"Woods","year":"2014","journal-title":"Adv Exp Med Biol"},{"key":"2022072511584691100_ref15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-3-030-15950-4_1","article-title":"Mass spectrometry for proteomics-based investigation","volume":"1140","author":"Woods","year":"2019","journal-title":"Adv Exp Med Biol"},{"key":"2022072511584691100_ref16","doi-asserted-by":"crossref","first-page":"4007","DOI":"10.1016\/S0021-9258(19)41496-8","article-title":"High resolution two-dimensional electrophoresis of proteins","volume":"250","author":"O'Farrell","year":"1975","journal-title":"J Biol Chem"},{"key":"2022072511584691100_ref17","doi-asserted-by":"crossref","DOI":"10.1016\/j.jpba.2020.113609","article-title":"Application for proteomics analysis technology in studying animal-derived traditional Chinese medicine: a review","volume":"191","author":"Tang","year":"2020","journal-title":"J Pharm Biomed Anal"},{"key":"2022072511584691100_ref18","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.jchromb.2008.01.029","article-title":"Recent development of multi-dimensional chromatography strategies in proteome research","volume":"866","author":"Tang","year":"2008","journal-title":"J Chromatogr B Analyt Technol Biomed Life Sci"},{"key":"2022072511584691100_ref19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.aca.2012.04.010","article-title":"Recent advances on multidimensional liquid chromatography-mass spectrometry for proteomics: from qualitative to quantitative analysis\u2014a review","volume":"731","author":"Wu","year":"2012","journal-title":"Anal Chim Acta"},{"key":"2022072511584691100_ref20","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1007\/s002160051561","article-title":"Nano-electrospray ionization mass spectrometry: addressing analytical problems beyond routine","volume":"366","author":"Karas","year":"2000","journal-title":"Fresenius J Anal Chem"},{"key":"2022072511584691100_ref21","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1007\/s00216-007-1486-6","article-title":"Quantitative mass spectrometry in proteomics: a critical review","volume":"389","author":"Bantscheff","year":"2007","journal-title":"Anal Bioanal Chem"},{"key":"2022072511584691100_ref22","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1515\/hsz-2017-0104","article-title":"Strategies in relative and absolute quantitative mass spectrometry based proteomics","volume":"398","author":"Lindemann","year":"2017","journal-title":"Biol Chem"},{"key":"2022072511584691100_ref23","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1126\/science.1124619","article-title":"Mass spectrometry and protein analysis","volume":"312","author":"Domon","year":"2006","journal-title":"Science"},{"key":"2022072511584691100_ref24","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.jpba.2015.04.013","article-title":"Stable isotope labelling methods in mass spectrometry-based quantitative proteomics","volume":"113","author":"Chahrour","year":"2015","journal-title":"J Pharm Biomed Anal"},{"key":"2022072511584691100_ref25","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1038\/msb.2008.61","article-title":"Selected reaction monitoring for quantitative proteomics: a tutorial","volume":"4","author":"Lange","year":"2008","journal-title":"Mol Syst Biol"},{"key":"2022072511584691100_ref26","doi-asserted-by":"crossref","DOI":"10.1155\/2013\/180605","article-title":"Current status and advances in quantitative proteomic mass spectrometry","volume":"2013","author":"Wasinger","year":"2013","journal-title":"Int J Proteomics"},{"key":"2022072511584691100_ref27","doi-asserted-by":"crossref","first-page":"1475","DOI":"10.1074\/mcp.O112.020131","article-title":"Parallel reaction monitoring for high resolution and high mass accuracy quantitative, targeted proteomics","volume":"11","author":"Peterson","year":"2012","journal-title":"Mol Cell Proteomics"},{"key":"2022072511584691100_ref28","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1146\/annurev-anchem-061516-045357","article-title":"Relative and absolute quantitation in mass spectrometry-based proteomics","volume":"11","author":"Ankney","year":"2018","journal-title":"Annu Rev Anal Chem"},{"key":"2022072511584691100_ref29","doi-asserted-by":"crossref","first-page":"7342","DOI":"10.1002\/anie.200501023","article-title":"Protein posttranslational modifications: the chemistry of proteome diversifications","volume":"44","author":"Walsh","year":"2005","journal-title":"Angew Chem Int Ed Engl"},{"key":"2022072511584691100_ref30","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1021\/ar020143l","article-title":"The characterization of protein post-translational modifications by mass spectrometry","volume":"36","author":"Schweppe","year":"2003","journal-title":"Acc Chem Res"},{"key":"2022072511584691100_ref31","doi-asserted-by":"crossref","first-page":"1630","DOI":"10.1038\/nprot.2008.150","article-title":"The SCX\/IMAC enrichment approach for global phosphorylation analysis by mass spectrometry","volume":"3","author":"Villen","year":"2008","journal-title":"Nat Protoc"},{"key":"2022072511584691100_ref32","doi-asserted-by":"crossref","first-page":"3935","DOI":"10.1021\/ac0498617","article-title":"Selective isolation at the femtomole level of phosphopeptides from proteolytic digests using 2D-NanoLC-ESI-MS\/MS and titanium oxide precolumns","volume":"76","author":"Pinkse","year":"2004","journal-title":"Anal Chem"},{"key":"2022072511584691100_ref33","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.aca.2018.09.066","article-title":"Sol-gel preparation of titanium (IV)-immobilized hierarchically porous organosilica hybrid monoliths","volume":"1046","author":"Zhang","year":"2019","journal-title":"Anal Chim Acta"},{"key":"2022072511584691100_ref34","doi-asserted-by":"crossref","first-page":"4655","DOI":"10.1021\/acs.analchem.7b00242","article-title":"Facile preparation of titanium(IV)-immobilized hierarchically porous hybrid monoliths","volume":"89","author":"Zhang","year":"2017","journal-title":"Anal Chem"},{"key":"2022072511584691100_ref35","doi-asserted-by":"crossref","first-page":"868","DOI":"10.1038\/nbt.1654","article-title":"Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling","volume":"28","author":"Xu","year":"2010","journal-title":"Nat Biotechnol"},{"key":"2022072511584691100_ref36","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1016\/j.cell.2010.04.012","article-title":"Precision mapping of an in vivo N-glycoproteome reveals rigid topological and sequence constraints","volume":"141","author":"Zielinska","year":"2010","journal-title":"Cell"},{"key":"2022072511584691100_ref37","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1016\/j.molcel.2006.06.026","article-title":"Substrate and functional diversity of lysine acetylation revealed by a proteomics survey","volume":"23","author":"Kim","year":"2006","journal-title":"Mol Cell"},{"key":"2022072511584691100_ref38","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1038\/nmeth715","article-title":"Identifying and quantifying in vivo methylation sites by heavy methyl SILAC","volume":"1","author":"Ong","year":"2004","journal-title":"Nat Methods"},{"key":"2022072511584691100_ref39","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1074\/mcp.M113.035600","article-title":"Analysis of the human tissue-specific expression by genome-wide integration of transcriptomics and antibody-based proteomics","volume":"13","author":"Fagerberg","year":"2014","journal-title":"Mol Cell Proteomics"},{"key":"2022072511584691100_ref40","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1002\/pmic.201200489","article-title":"Scanning of novel cancer\/testis proteins by human testis proteomic analysis","volume":"13","author":"Liu","year":"2013","journal-title":"Proteomics"},{"key":"2022072511584691100_ref41","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.jprot.2012.12.008","article-title":"In-depth proteomic analysis of the human sperm reveals complex protein compositions","volume":"79","author":"Wang","year":"2013","journal-title":"J Proteomics"},{"key":"2022072511584691100_ref42","doi-asserted-by":"crossref","first-page":"2583","DOI":"10.1111\/jcmm.14149","article-title":"Deficiency of TPPP2, a factor linked to oligoasthenozoospermia, causes subfertility in male mice","volume":"23","author":"Zhu","year":"2019","journal-title":"J Cell Mol Med"},{"key":"2022072511584691100_ref43","doi-asserted-by":"crossref","first-page":"E5370","DOI":"10.1073\/pnas.1621279114","article-title":"TCTE1 is a conserved component of the dynein regulatory complex and is required for motility and metabolism in mouse spermatozoa","volume":"114","author":"Castaneda","year":"2017","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2022072511584691100_ref44","doi-asserted-by":"crossref","first-page":"2040","DOI":"10.1111\/jcmm.16116","article-title":"PRSS55 plays an important role in the structural differentiation and energy metabolism of sperm and is required for male fertility in mice","volume":"25","author":"Zhu","year":"2021","journal-title":"J Cell Mol Med"},{"key":"2022072511584691100_ref45","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1093\/biolre\/ioaa018","article-title":"LYPD4, mouse homolog of a human acrosome protein, is essential for sperm fertilizing ability and male fertility","volume":"102","author":"Wang","year":"2020","journal-title":"Biol Reprod"},{"key":"2022072511584691100_ref46","doi-asserted-by":"crossref","DOI":"10.1093\/database\/baab012","article-title":"Posttranslational modifications in proteins: resources, tools and prediction methods","volume":"2021","author":"Ramazi","year":"2021","journal-title":"Database (Oxford)"},{"key":"2022072511584691100_ref47","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1007\/s00441-015-2249-x","article-title":"Proteomics of post-translational modifications of mammalian spermatozoa","volume":"363","author":"Baker","year":"2016","journal-title":"Cell Tissue Res"},{"key":"2022072511584691100_ref48","first-page":"75","article-title":"Advances in proteomic studies of post-translational modifications during spermatogenesis","volume":"72","author":"Zhu","year":"2020","journal-title":"Sheng Li Xue Bao"},{"key":"2022072511584691100_ref49","doi-asserted-by":"crossref","first-page":"E127","DOI":"10.1038\/ncb0502-e127","article-title":"The origins of protein phosphorylation","volume":"4","author":"Cohen","year":"2002","journal-title":"Nat Cell Biol"},{"key":"2022072511584691100_ref50","doi-asserted-by":"crossref","first-page":"5375","DOI":"10.1021\/pr900659n","article-title":"Effect of peptide-to-TiO2 beads ratio on phosphopeptide enrichment selectivity","volume":"8","author":"Li","year":"2009","journal-title":"J Proteome Res"},{"key":"2022072511584691100_ref51","doi-asserted-by":"crossref","first-page":"3626","DOI":"10.1074\/mcp.M114.039073","article-title":"Systematic analysis of the phosphoproteome and kinase-substrate networks in the mouse testis","volume":"13","author":"Qi","year":"2014","journal-title":"Mol Cell Proteomics"},{"key":"2022072511584691100_ref52","doi-asserted-by":"crossref","first-page":"S132","DOI":"10.1074\/mcp.RA118.001278","article-title":"Human testis phosphoproteome reveals kinases as potential targets in spermatogenesis and testicular cancer","volume":"18","author":"Castillo","year":"2019","journal-title":"Mol Cell Proteomics"},{"key":"2022072511584691100_ref53","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1046\/j.1365-2613.1998.00057.x","article-title":"Stem cells in the testis","volume":"79","author":"Rooij","year":"1998","journal-title":"Int J Exp Pathol"},{"key":"2022072511584691100_ref54","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1074\/mcp.M116.065797","article-title":"The glial cell-derived neurotrophic factor (GDNF)-responsive phosphoprotein landscape identifies raptor phosphorylation required for spermatogonial progenitor cell proliferation","volume":"16","author":"Wang","year":"2017","journal-title":"Mol Cell Proteomics"},{"key":"2022072511584691100_ref55","doi-asserted-by":"crossref","first-page":"1835","DOI":"10.1242\/jcs.202721","article-title":"TSSK6 is required for gammaH2AX formation and the histone-to-protamine transition during spermiogenesis","volume":"130","author":"Jha","year":"2017","journal-title":"J Cell Sci"},{"key":"2022072511584691100_ref56","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1074\/mcp.M114.045468","article-title":"Quantitative phosphoproteomics analysis reveals a key role of insulin growth factor 1 receptor (IGF1R) tyrosine kinase in human sperm capacitation","volume":"14","author":"Wang","year":"2015","journal-title":"Mol Cell Proteomics"},{"key":"2022072511584691100_ref57","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1038\/nrm2780","article-title":"Building ubiquitin chains: E2 enzymes at work","volume":"10","author":"Ye","year":"2009","journal-title":"Nat Rev Mol Cell Biol"},{"key":"2022072511584691100_ref58","doi-asserted-by":"crossref","DOI":"10.1002\/pmic.202100025","article-title":"Systematic analysis of the ubiquitome in mouse testis","volume":"21","author":"Guo","year":"2021","journal-title":"Proteomics"},{"key":"2022072511584691100_ref59","doi-asserted-by":"crossref","first-page":"1144","DOI":"10.1074\/mcp.M112.020123","article-title":"Integrative proteomic and transcriptomic analyses reveal multiple post-transcriptional regulatory mechanisms of mouse spermatogenesis","volume":"12","author":"Gan","year":"2013","journal-title":"Mol Cell Proteomics"},{"key":"2022072511584691100_ref60","doi-asserted-by":"crossref","first-page":"10457","DOI":"10.1007\/s00253-018-9430-6","article-title":"Glycosylation control technologies for recombinant therapeutic proteins","volume":"102","author":"Gupta","year":"2018","journal-title":"Appl Microbiol Biotechnol"},{"key":"2022072511584691100_ref61","doi-asserted-by":"crossref","first-page":"8983","DOI":"10.1073\/pnas.0603248103","article-title":"Essential and mutually compensatory roles of {alpha}-mannosidase II and {alpha}-mannosidase IIx in N-glycan processing in vivo in mice","volume":"103","author":"Akama","year":"2006","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2022072511584691100_ref62","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1095\/biolreprod.114.121095","article-title":"Dynamic changes in equatorial segment protein 1 (SPESP1) glycosylation during mouse spermiogenesis","volume":"92","author":"Suryavathi","year":"2015","journal-title":"Biol Reprod"},{"key":"2022072511584691100_ref63","doi-asserted-by":"crossref","first-page":"3877","DOI":"10.1021\/acs.jproteome.9b00795","article-title":"Biological functions and large-scale profiling of protein glycosylation in human semen","volume":"19","author":"Lan","year":"2020","journal-title":"J Proteome Res"},{"key":"2022072511584691100_ref64","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1007\/s00018-003-2339-x","article-title":"The in vivo role of alpha-mannosidase IIx and its role in processing of N-glycans in spermatogenesis","volume":"60","author":"Fukuda","year":"2003","journal-title":"Cell Mol Life Sci"},{"key":"2022072511584691100_ref65","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1016\/S0304-4165(02)00407-5","article-title":"In vivo role of alpha-mannosidase IIx: ineffective spermatogenesis resulting from targeted disruption of the Man2a2 in the mouse","volume":"1573","author":"Fukuda","year":"2002","journal-title":"Biochim Biophys Acta"},{"key":"2022072511584691100_ref66","doi-asserted-by":"crossref","first-page":"5750","DOI":"10.1021\/pr400753f","article-title":"Mapping of the N-linked glycoproteome of human spermatozoa","volume":"12","author":"Wang","year":"2013","journal-title":"J Proteome Res"},{"key":"2022072511584691100_ref67","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1093\/humupd\/5.4.302","article-title":"The glycocalyx of the sperm surface","volume":"5","author":"Schroter","year":"1999","journal-title":"Hum Reprod Update"},{"key":"2022072511584691100_ref68","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1002\/pmic.201400203","article-title":"Proteomic analysis of N-glycosylation of human seminal plasma","volume":"15","author":"Yang","year":"2015","journal-title":"Proteomics"},{"key":"2022072511584691100_ref69","article-title":"Lysine acetylation of cytoskeletal proteins: emergence of an actin code","volume":"219","author":"Mu","year":"2020","journal-title":"J Cell Biol"},{"key":"2022072511584691100_ref70","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1038\/417455a","article-title":"HDAC6 is a microtubule-associated deacetylase","volume":"417","author":"Hubbert","year":"2002","journal-title":"Nature"},{"key":"2022072511584691100_ref71","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/S1097-2765(03)00038-8","article-title":"The human Sir2 ortholog, SIRT2, is an NAD+-dependent tubulin deacetylase","volume":"11","author":"North","year":"2003","journal-title":"Mol Cell"},{"key":"2022072511584691100_ref72","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1083\/jcb.200205057","article-title":"The human silent information regulator (Sir)2 homologue hSIRT3 is a mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase","volume":"158","author":"Schwer","year":"2002","journal-title":"J Cell Biol"},{"key":"2022072511584691100_ref73","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.jprot.2014.07.002","article-title":"Insights into the lysine acetylproteome of human sperm","volume":"109","author":"Sun","year":"2014","journal-title":"J Proteomics"},{"key":"2022072511584691100_ref74","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2021.769167","article-title":"The role of E3 ubiquitin ligases and deubiquitinases in inflammatory bowel disease: friend or foe?","volume":"12","author":"Zou","year":"2021","journal-title":"Front Immunol"},{"key":"2022072511584691100_ref75","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1002\/msb.201304521","article-title":"Evolution and functional cross-talk of protein post-translational modifications","volume":"9","author":"Beltrao","year":"2013","journal-title":"Mol Syst Biol"},{"key":"2022072511584691100_ref76","doi-asserted-by":"crossref","DOI":"10.1016\/j.mcpro.2021.100129","article-title":"Decoding post-translational modification crosstalk with proteomics","volume":"20","author":"Leutert","year":"2021","journal-title":"Mol Cell Proteomics"},{"key":"2022072511584691100_ref77","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.apsb.2020.09.007","article-title":"Phosphorylation regulates cullin-based ubiquitination in tumorigenesis","volume":"11","author":"Chen","year":"2021","journal-title":"Acta Pharm Sin B"},{"key":"2022072511584691100_ref78","doi-asserted-by":"crossref","first-page":"3155","DOI":"10.1073\/pnas.1817281116","article-title":"Calcineurin dephosphorylates Kelch-like 3, reversing phosphorylation by angiotensin II and regulating renal electrolyte handling","volume":"116","author":"Ishizawa","year":"2019","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2022072511584691100_ref79","doi-asserted-by":"crossref","first-page":"20603","DOI":"10.1073\/pnas.1102572108","article-title":"Cullin 3 mediates SRC-3 ubiquitination and degradation to control the retinoic acid response","volume":"108","author":"Ferry","year":"2011","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2022072511584691100_ref80","doi-asserted-by":"crossref","DOI":"10.1016\/j.jprot.2019.103478","article-title":"Proteomics analysis of asthenozoospermia and identification of glucose-6-phosphate isomerase as an important enzyme for sperm motility","volume":"208","author":"Guo","year":"2019","journal-title":"J Proteomics"},{"key":"2022072511584691100_ref81","doi-asserted-by":"crossref","DOI":"10.1002\/prca.201900007","article-title":"Proteomic analysis of Dpy19l2-deficient human globozoospermia reveals multiple molecular defects","volume":"13","author":"Guo","year":"2019","journal-title":"Proteomics Clin Appl"},{"key":"2022072511584691100_ref82","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1002\/prca.201200078","article-title":"Differential proteomic profiling in human spermatozoa that did or did not result in pregnancy via IVF and AID","volume":"7","author":"Zhu","year":"2013","journal-title":"Proteomics Clin Appl"},{"key":"2022072511584691100_ref83","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1016\/j.fertnstert.2018.01.043","article-title":"Evaluation of the azoospermic male: a committee opinion","volume":"109","author":"Practice Committee of the American Society for Reproductive Medicine in collaboration with the Society for Male Reproduction and Urology","year":"2018","journal-title":"Fertil Steril"},{"key":"2022072511584691100_ref84","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1093\/humrep\/dey072","article-title":"Exosomal microRNAs in seminal plasma are markers of the origin of azoospermia and can predict the presence of sperm in testicular tissue","volume":"33","author":"Barcelo","year":"2018","journal-title":"Hum Reprod"},{"key":"2022072511584691100_ref85","doi-asserted-by":"crossref","first-page":"1736935","DOI":"10.1080\/20013078.2020.1736935","article-title":"Unravelling the proteomic landscape of extracellular vesicles in prostate cancer by density-based fractionation of urine","volume":"9","author":"Dhondt","year":"2020","journal-title":"J Extracell Vesicles"},{"key":"2022072511584691100_ref86","doi-asserted-by":"crossref","DOI":"10.1002\/ctm2.423","article-title":"Quantitative proteomic biomarkers from extracellular vesicles of human seminal plasma in the differential diagnosis of azoospermia","volume":"11","author":"Yao","year":"2021","journal-title":"Clin Transl Med"},{"key":"2022072511584691100_ref87","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1038\/d41586-021-01994-w","article-title":"Single-cell analysis enters the multiomics age","volume":"595","author":"Perkel","year":"2021","journal-title":"Nature"},{"key":"2022072511584691100_ref88","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1038\/s41422-018-0074-y","article-title":"Single-cell RNA-seq uncovers dynamic processes and critical regulators in mouse spermatogenesis","volume":"28","author":"Chen","year":"2018","journal-title":"Cell Res"},{"key":"2022072511584691100_ref89","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1016\/j.stem.2018.08.007","article-title":"Single-cell RNA sequencing analysis reveals sequential cell fate transition during human spermatogenesis","volume":"23","author":"Wang","year":"2018","journal-title":"Cell Stem Cell"},{"key":"2022072511584691100_ref90","doi-asserted-by":"crossref","DOI":"10.1002\/cpz1.118","article-title":"CyTOF: an emerging technology for single-cell proteomics in the mouse","volume":"1","author":"Tracey","year":"2021","journal-title":"Curr Protoc"},{"key":"2022072511584691100_ref91","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1038\/s41467-018-03367-w","article-title":"Nanodroplet processing platform for deep and quantitative proteome profiling of 10-100 mammalian cells","volume":"9","author":"Zhu","year":"2018","journal-title":"Nat Commun"},{"key":"2022072511584691100_ref92","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1186\/s13059-018-1547-5","article-title":"SCoPE-MS: mass spectrometry of single mammalian cells quantifies proteome heterogeneity during cell differentiation","volume":"19","author":"Budnik","year":"2018","journal-title":"Genome Biol"},{"key":"2022072511584691100_ref93","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1038\/s41570-020-0162-7","article-title":"Single-cell analysis targeting the proteome","volume":"4","author":"Labib","year":"2020","journal-title":"Nat Rev Chem"},{"key":"2022072511584691100_ref94","first-page":"194","article-title":"The ICSI procedure from past to future: a systematic review of the more controversial aspects","volume":"22","author":"Rubino","year":"2016","journal-title":"Hum Reprod Update"},{"key":"2022072511584691100_ref95","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1093\/humupd\/dmv042","article-title":"Sperm selection in natural conception: what can we learn from mother nature to improve assisted reproduction outcomes?","volume":"21","author":"Sakkas","year":"2015","journal-title":"Hum Reprod Update"},{"key":"2022072511584691100_ref96","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1016\/S1472-6483(10)61191-1","article-title":"How to improve IVF-ICSI outcome by sperm selection","volume":"12","author":"Berkovitz","year":"2006","journal-title":"Reprod Biomed Online"}],"container-title":["Biology of Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/biolreprod\/advance-article-pdf\/doi\/10.1093\/biolre\/ioac065\/43447725\/ioac065.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/biolreprod\/article-pdf\/107\/1\/109\/45055942\/ioac065.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/biolreprod\/article-pdf\/107\/1\/109\/45055942\/ioac065.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,21]],"date-time":"2024-09-21T13:55:56Z","timestamp":1726926956000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/biolreprod\/article\/107\/1\/109\/6562766"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,2]]},"references-count":96,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2022,4,2]]},"published-print":{"date-parts":[[2022,7,25]]}},"URL":"https:\/\/doi.org\/10.1093\/biolre\/ioac065","relation":{},"ISSN":["0006-3363","1529-7268"],"issn-type":[{"value":"0006-3363","type":"print"},{"value":"1529-7268","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2022,7]]},"published":{"date-parts":[[2022,4,2]]}}}