{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T15:00:43Z","timestamp":1781967643965,"version":"3.54.5"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2022,4,26]],"date-time":"2022-04-26T00:00:00Z","timestamp":1650931200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Research Area Network, on Sustainable Animal Production","award":["16\/RD\/SusAn\/ERA-NET"],"award-info":[{"award-number":["16\/RD\/SusAn\/ERA-NET"]}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-16-SUSN-0001"],"award-info":[{"award-number":["ANR-16-SUSN-0001"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005416","name":"Research Council of Norway","doi-asserted-by":"publisher","award":["272338 \/ E50"],"award-info":[{"award-number":["272338 \/ E50"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,8,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Sialic acid occupies terminal positions on O-glycans of cervical mucins, where they contribute to the increased viscosity of mucin thereby regulating sperm transport. This study characterized the sialylated cervical mucins from follicular phase mucus of six European ewe breeds with known differences in pregnancy rates following cervical artificial insemination (AI) using frozen\u2013thawed semen at both synchronized and natural estrus cycles. These were Suffolk (low fertility) and Belclare (medium fertility) in Ireland, Ile de France and Romanov (both with medium fertility) in France, and Norwegian White Sheep (NWS) and Fur (both with high fertility) in Norway. Expression of mucin and sialic acid related genes was quantified using RNA-sequencing in cervical tissue from Suffolk, Belclare, Fur, and NWS only. Cervical tissue was also assessed for the percentage of cervical epithelial populated by mucin secreting goblet cells in the same four ewe breeds. Biochemical analysis showed that there was an effect of ewe breed on sialic acid species, which was represented by Suffolk having higher levels of Neu5,9Ac2 compared with NWS (P\u2009&amp;lt;\u20090.05). Suffolk ewes had a lower percentage of goblet cells than Fur and NWS (P\u2009&amp;lt;\u20090.05). Gene expression analysis identified higher expression of MUC5AC, MUC5B, ST6GAL1, and ST6GAL2 and lower expression of ST3GAL3, ST3GAL4, and SIGLEC10 in Suffolk compared with high fertility ewe breeds (P\u2009&amp;lt;\u20090.05). Our results indicate that specific alterations in sialylated mucin composition may be related to impaired cervical sperm transport.<\/jats:p>","DOI":"10.1093\/biolre\/ioac077","type":"journal-article","created":{"date-parts":[[2022,4,26]],"date-time":"2022-04-26T11:12:59Z","timestamp":1650971579000},"page":"419-431","source":"Crossref","is-referenced-by-count":18,"title":["Biochemical and molecular characterization of sialylated cervical mucins in sheep"],"prefix":"10.1093","volume":"107","author":[{"given":"Laura","family":"Abril-Parre\u00f1o","sequence":"first","affiliation":[{"name":"Laboratory of Animal Reproduction, Department of Biological Sciences, School of Natural Sciences, Biomaterials Research Cluster, Bernal Institute, Faculty of Science and Engineering, University of Limerick , Limerick, V94 T9PX , Ireland"},{"name":"Animal & Bioscience Research Department, Teagasc, Animal & Grassland Research and Innovation Centre , Grange, C15 PW93 , Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jack","family":"Morgan","sequence":"additional","affiliation":[{"name":"NIBRT GlycoScience Group, The National Institute for Bioprocessing Research and Training , Blackrock, Dublin, A94 X099 , Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anette","family":"Krogen\u00e6s","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Production Animal Clinical Sciences, Norwegian University of Life Sciences , \u00c5s 5003 1432 , Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Druart","sequence":"additional","affiliation":[{"name":"UMR-PRC, INRA-85, Universit\u00e9 de Tours, IFCE, Department of Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique , Nouzilly 37380 , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Cormican","sequence":"additional","affiliation":[{"name":"Animal & Bioscience Research Department, Teagasc, Animal & Grassland Research and Innovation Centre , Grange, C15 PW93 , Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary E","family":"Gallagher","sequence":"additional","affiliation":[{"name":"UCD Veterinary Sciences Centre, University College Dublin , Belfield, Dublin 4, D04 W6F6 , Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colm","family":"Reid","sequence":"additional","affiliation":[{"name":"UCD Veterinary Sciences Centre, University College Dublin , Belfield, Dublin 4, D04 W6F6 , Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kieran","family":"Meade","sequence":"additional","affiliation":[{"name":"UCD School of Agriculture and Food Science, University College Dublin , Belfield, Dublin 4, D04 W6F6 , Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radka","family":"Saldova","sequence":"additional","affiliation":[{"name":"NIBRT GlycoScience Group, The National Institute for Bioprocessing Research and Training , Blackrock, Dublin, A94 X099 , Ireland"},{"name":"UCD School of Medicine, College of Health and Agricultural Science, University College Dublin , Belfield, Dublin 4, D07 A8NN , Ireland"},{"name":"C\u00daRAM, SFI Research Centre for Medical Devices, National University of Ireland , Galway, H91 W2TY , Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean","family":"Fair","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Reproduction, Department of Biological Sciences, School of Natural Sciences, Biomaterials Research Cluster, Bernal Institute, Faculty of Science and Engineering, University of Limerick , Limerick, V94 T9PX , Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,4,26]]},"reference":[{"key":"2022081707383029700_ref1","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S0015-0282(16)44539-5","article-title":"A new quantitative test for sperm penetration into cervical mucus","volume":"33","author":"Katz","year":"1980","journal-title":"Fertil Steril"},{"key":"2022081707383029700_ref2","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1146\/annurev.physiol.70.113006.100702","article-title":"Structure and function of the polymeric mucins in airways mucus","volume":"70","author":"Thornton","year":"2008","journal-title":"Annu Rev Physiol"},{"key":"2022081707383029700_ref3","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1152\/physiolgenomics.00088.2012","article-title":"Molecular aspects of mucin biosynthesis and mucus formation in the bovine cervix during the periestrous period","volume":"44","author":"Pluta","year":"2012","journal-title":"Physiol Genomics"},{"key":"2022081707383029700_ref4","doi-asserted-by":"crossref","first-page":"D1245","DOI":"10.2741\/Gipson","article-title":"Mucins of the human endocervix","volume":"6","author":"Gipson","year":"2001","journal-title":"Front Biosci"},{"key":"2022081707383029700_ref5","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1074\/mcp.M600439-MCP200","article-title":"Large scale identification of proteins, mucins, and their O-glycosylation in the endocervical mucus during the menstrual cycle","volume":"6","author":"Andersch-Bj\u00f6rkman","year":"2007","journal-title":"Mol Cell Proteomics"},{"key":"2022081707383029700_ref6","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a014159","article-title":"Perspectives on mucus properties and formation--lessons from the biochemical world","volume":"2","author":"Ambort","year":"2012","journal-title":"Cold Spring Harb Perspect 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extracellular traps in bovine in vitro model","volume":"10","author":"Bornh\u00f6fft","year":"2019","journal-title":"Front Immunol"},{"key":"2022081707383029700_ref11","doi-asserted-by":"crossref","first-page":"R1","DOI":"10.1530\/REP-18-0595","article-title":"The biological mechanisms regulating sperm selection by the ovine cervix","volume":"158","author":"Fair","year":"2019","journal-title":"Reproduction"},{"key":"2022081707383029700_ref12","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/S0378-4320(00)00155-X","article-title":"Storage of ram semen","volume":"62","author":"Salamon","year":"2000","journal-title":"Anim Reprod Sci"},{"key":"2022081707383029700_ref13","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/1751-0147-49-26","article-title":"Comparison of fertility results after vaginal insemination using different thawing procedures and packages for frozen ram semen","volume":"49","author":"Paulenz","year":"2007","journal-title":"Acta Vet 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mucus","volume":"172","author":"Maddison","year":"2016","journal-title":"Anim Reprod Sci"},{"key":"2022081707383029700_ref29","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1530\/REP-17-0475","article-title":"Siglec expression on the surface of human, bull and ram sperm","volume":"155","author":"Alkhodair","year":"2018","journal-title":"Reproduction"},{"key":"2022081707383029700_ref30","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1095\/biolreprod.115.137810","article-title":"Sialylation facilitates the maturation of mammalian sperm and affects its survival in female uterus","volume":"94","author":"Ma","year":"2016","journal-title":"Biol Reprod"},{"key":"2022081707383029700_ref31","doi-asserted-by":"crossref","first-page":"233","DOI":"10.4103\/2096-2924.224914","article-title":"Effects of cryopreservation on human sperm glycocalyx","volume":"1","author":"Wu","year":"2017","journal-title":"Reprod Dev 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responses","volume":"323","author":"Chen","year":"2009","journal-title":"Science"},{"key":"2022081707383029700_ref35","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1038\/cr.2015.108","article-title":"Siglec1 suppresses antiviral innate immune response by inducing TBK1 degradation via the ubiquitin ligase TRIM27","volume":"25","author":"Zheng","year":"2015","journal-title":"Cell Res"}],"container-title":["Biology of 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