{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T19:08:45Z","timestamp":1770491325598,"version":"3.49.0"},"reference-count":77,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2022,4,29]],"date-time":"2022-04-29T00:00:00Z","timestamp":1651190400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U54MD007601"],"award-info":[{"award-number":["U54MD007601"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"UTokyo Global Activity Support Program for Young Researchers"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,9,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Prssly (Protease, serine-like, Chr Y) and Teyorf1 (Testis expressed, chromosome Y open reading frame 1) are two acquired single-copy genes located on the distal tip of the non-pairing short arm of the mouse Y chromosome adjacent to telomeric sequence. Both genes lack X chromosome-linked homologues and are expressed in testicular germ cells. We first performed analysis of Prssly and Teyorf1 genomic sequences and demonstrated that previously reported Prssly sequence is erroneous and the true Prssly sequence is longer and encodes a larger protein than previously estimated. We also confirmed that both genes encode pseudogenes that are not expressed in testes. Next, using CRISPR\/Cas9 genome targeting, we generated Prssly and Teyorf1 knockout (KO) mice and characterized their phenotype. To create Prssly KO mice, we targeted the conserved exon 5 encoding a trypsin domain typical for serine proteases. The targeting was successful and resulted in a frame shift mutation that introduced a premature stop codon, with the Prssly KO males retaining only residual transcript expression in testes. The Teyorf1 targeting removed the entire open reading frame of the gene, which resulted in no transcript expression in KO males. Both Prssly KO and Teyorf1 KO males were fertile and had normal testis size and normal sperm number, motility, and morphology. Our findings show that Prssly and Teyorf1 transcripts with potential to encode proteins are dispensable for male fertility.<\/jats:p>","DOI":"10.1093\/biolre\/ioac084","type":"journal-article","created":{"date-parts":[[2022,4,29]],"date-time":"2022-04-29T10:36:12Z","timestamp":1651228572000},"page":"752-764","source":"Crossref","is-referenced-by-count":11,"title":["Two acquired mouse Y chromosome-linked genes,<i>Prssl<\/i>y and<i>Teyorf1<\/i>, are dispensable for male fertility"],"prefix":"10.1093","volume":"107","author":[{"given":"Hayden","family":"Holmlund","sequence":"first","affiliation":[{"name":"Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii , Honolulu, HI , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhiro","family":"Yamauchi","sequence":"additional","affiliation":[{"name":"Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii , Honolulu, HI , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerald","family":"Durango","sequence":"additional","affiliation":[{"name":"Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii , Honolulu, HI , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wataru","family":"Fujii","sequence":"additional","affiliation":[{"name":"Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii , Honolulu, HI , USA"},{"name":"Graduate School of Agricultural and Life Sciences, University of Tokyo , Tokyo , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika A","family":"Ward","sequence":"additional","affiliation":[{"name":"Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii , Honolulu, HI , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,4,29]]},"reference":[{"key":"2022091511312830400_ref1","doi-asserted-by":"crossref","first-page":"800","DOI":"10.1016\/j.cell.2014.09.052","article-title":"Sequencing the mouse y chromosome reveals convergent gene acquisition and amplification on both sex chromosomes","volume":"159","author":"Soh","year":"2014","journal-title":"Cell"},{"key":"2022091511312830400_ref2","doi-asserted-by":"crossref","first-page":"e0145398","DOI":"10.1371\/journal.pone.0145398","article-title":"Mouse Y-encoded transcription factor Zfy2 is essential for sperm head remodelling and sperm tail development","volume":"11","author":"Vernet","year":"2016","journal-title":"PLoS One"},{"key":"2022091511312830400_ref3","doi-asserted-by":"crossref","first-page":"e1005476","DOI":"10.1371\/journal.pgen.1005476","article-title":"Mouse Y-encoded 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