{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T17:42:20Z","timestamp":1791222140756,"version":"4.1.0"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2022,5,11]],"date-time":"2022-05-11T00:00:00Z","timestamp":1652227200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82173550"],"award-info":[{"award-number":["82173550"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81973080"],"award-info":[{"award-number":["81973080"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Priority Academic Program for the Development of Jiangsu Higher Education Institutions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,7,25]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Triclosan is a broad-spectrum antibacterial agent and widely exists in environmental media and organisms. Triclosan exposure has been reported to have adverse effects on reproduction including embryo implantation disorder. During the embryo implantation window, it is vital that the endometrium develops into a receptive state under the influence of ovarian hormones. However, the effect of triclosan on embryo implantation and endometrial receptivity remains unclear. In the current study, we found a decreased embryo implantation rate, serum estrogen, and progesterone levels in mice exposed to triclosan from gestation days 0.5 to 5.5. Through RNA sequencing (RNA-seq), we identified nearly 800 differentially expressed genes, which were enriched in various pathways, including uterus development, inflammatory response, and immune system processes. Among those enriched pathways, the tight junction pathway is essential for the establishment of the receptive state of the endometrium. Then, genes involved in the tight junction pathway, including Cldn7, Cldn10, and Crb3, were validated by quantitative real-time polymerase chain reaction and the results were consistent with those from RNA-seq. Through immunofluorescence staining and western blotting, we confirmed that the tight junction protein levels of CLDN7 and CRB3 were increased. All these findings suggest that preimplantation triclosan exposure reduces the rate of embryo implantation through upregulating the expression of the tight junction genes and affecting the receptivity of the endometrium. Our data could be used to determine the sensitive time frame for triclosan exposure and offer a new strategy to prevent implantation failure.<\/jats:p>","DOI":"10.1093\/biolre\/ioac092","type":"journal-article","created":{"date-parts":[[2022,5,12]],"date-time":"2022-05-12T14:00:37Z","timestamp":1652364037000},"page":"349-357","source":"Crossref","is-referenced-by-count":10,"title":["Preimplantation triclosan exposure alters uterine receptivity through affecting tight junction protein"],"prefix":"10.1093","volume":"107","author":[{"given":"Guangzhu","family":"Dong","sequence":"first","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine , Center for Global Health, School of Public Health, , Nanjing , China"},{"name":"Nanjing Medical University , Center for Global Health, School of Public Health, , Nanjing , China"},{"name":"Key Laboratory of Modern Toxicology of Ministry of Education , School of Public Health, , Nanjing , China"},{"name":"Nanjing Medical University , School of Public Health, , Nanjing , China"},{"name":"Baijiahu Community Health Service Center , Nanjing , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rundong","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine , Center for Global Health, School of Public Health, , Nanjing , China"},{"name":"Nanjing Medical University , Center for Global Health, School of Public Health, , Nanjing , China"},{"name":"Key Laboratory of Modern Toxicology of Ministry of Education , School of Public Health, , Nanjing , China"},{"name":"Nanjing Medical University , School of Public Health, , Nanjing , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine , Center for Global Health, School of Public Health, , Nanjing , China"},{"name":"Nanjing Medical University , Center for Global Health, School of Public Health, , Nanjing , China"},{"name":"Key Laboratory of Modern Toxicology of Ministry of Education , School of Public Health, , Nanjing , China"},{"name":"Nanjing Medical University , School of Public Health, , Nanjing , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufeng","family":"Qin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Modern Toxicology of Ministry of Education , School of Public Health, , Nanjing , China"},{"name":"Nanjing Medical University , School of Public Health, , Nanjing , China"},{"name":"Department of Microbes and Infection , School of Public Health, , Nanjing , China"},{"name":"Nanjing Medical University , School of Public Health, , Nanjing , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuncheng","family":"Lu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine , Center for Global Health, School of Public Health, , Nanjing , China"},{"name":"Nanjing Medical University , Center for Global Health, School of Public Health, , Nanjing , China"},{"name":"Key Laboratory of Modern Toxicology of Ministry of Education , School of Public Health, , Nanjing , China"},{"name":"Nanjing Medical University , School of Public Health, , Nanjing , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinru","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine , Center for Global Health, School of Public Health, , Nanjing , China"},{"name":"Nanjing Medical University , Center for Global Health, School of Public Health, , Nanjing , China"},{"name":"Key Laboratory of Modern Toxicology of Ministry of Education , School of Public Health, , Nanjing , China"},{"name":"Nanjing Medical University , School of Public Health, , Nanjing , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yankai","family":"Xia","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine , Center for Global Health, School of Public Health, , Nanjing , China"},{"name":"Nanjing Medical University , Center for Global Health, School of Public Health, , Nanjing , China"},{"name":"Key Laboratory of Modern Toxicology of Ministry of Education , School of Public Health, , Nanjing , China"},{"name":"Nanjing Medical University , School of Public Health, , Nanjing , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guizhen","family":"Du","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine , Center for Global Health, School of Public Health, , Nanjing , China"},{"name":"Nanjing Medical University , Center for Global Health, School of Public Health, , Nanjing , China"},{"name":"Key Laboratory of Modern Toxicology of Ministry of Education , School of Public Health, , Nanjing , China"},{"name":"Nanjing Medical University , School of Public Health, , Nanjing , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,5,11]]},"reference":[{"key":"2022072512085861200_ref1","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1038\/nrm3758","article-title":"Molecular mechanisms of epithelial-mesenchymal transition","volume":"15","author":"Lamouille","year":"2014","journal-title":"Nat Rev Mol Cell Biol"},{"key":"2022072512085861200_ref2","first-page":"519","article-title":"Homocysteine and folate levels as risk factors for recurrent early pregnancy loss","volume":"95","author":"Nelen","year":"2000","journal-title":"Obstet Gynecol"},{"key":"2022072512085861200_ref3","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1080\/14789450.2018.1464915","article-title":"Does the dysregulation of matrix metalloproteinases contribute to recurrent implantation failure?","volume":"15","author":"Benkhalifa","year":"2018","journal-title":"Expert Rev Proteomics"},{"key":"2022072512085861200_ref4","doi-asserted-by":"crossref","first-page":"e81","DOI":"10.1016\/j.fertnstert.2007.12.036","article-title":"Science linking environmental contaminant exposures with fertility and reproductive health impacts in the adult female","volume":"89","author":"Mendola","year":"2008","journal-title":"Fertil Steril"},{"key":"2022072512085861200_ref5","doi-asserted-by":"crossref","first-page":"114763","DOI":"10.1016\/j.envpol.2020.114763","article-title":"Exposure of U.S. population to endocrine disruptive chemicals (parabens, benzophenone-3, bisphenol-A and triclosan) and their associations with female infertility","volume":"265","author":"Arya","year":"1987","journal-title":"Environ Pollut"},{"key":"2022072512085861200_ref6","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1289\/ehp.11200","article-title":"In vitro biologic activities of the antimicrobials triclocarban, its analogs, and triclosan in bioassay screens: receptor-based bioassay screens","volume":"116","author":"Ahn","year":"2008","journal-title":"Environ Health Perspect"},{"key":"2022072512085861200_ref7","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1080\/10937404.2017.1399306","article-title":"Triclosan exposure, transformation, and human health effects","volume":"20","author":"Weatherly","year":"2017","journal-title":"J Toxicol Environ Health B Crit Rev"},{"key":"2022072512085861200_ref8","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1016\/j.chemosphere.2011.03.042","article-title":"Biodegradation of triclosan and formation of methyl-triclosan in activated sludge under aerobic conditions","volume":"84","author":"Chen","year":"2011","journal-title":"Chemosphere"},{"key":"2022072512085861200_ref9","doi-asserted-by":"crossref","first-page":"115117","DOI":"10.1016\/j.envpol.2020.115117","article-title":"Triclosan and triclocarbon in maternal-fetal serum, urine, and amniotic fluid samples and their implication for prenatal exposure","volume":"266","author":"Bai","year":"1987","journal-title":"Environ Pollut"},{"key":"2022072512085861200_ref10","doi-asserted-by":"crossref","first-page":"1682","DOI":"10.1016\/j.chemosphere.2011.08.009","article-title":"Triclosan in individual human milk samples from Australia","volume":"85","author":"Toms","year":"2011","journal-title":"Chemosphere"},{"key":"2022072512085861200_ref11","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.cca.2016.12.024","article-title":"Triclosan\/triclocarban levels in maternal and umbilical blood samples and their association with fetal malformation","volume":"466","author":"Wei","year":"2017","journal-title":"Clinica Chimica Acta"},{"key":"2022072512085861200_ref12","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1289\/ehp.10768","article-title":"Urinary concentrations of triclosan in the U.S. population: 2003-2004","volume":"116","author":"Calafat","year":"2008","journal-title":"Environ Health Perspect"},{"key":"2022072512085861200_ref13","doi-asserted-by":"crossref","first-page":"S24","DOI":"10.1097\/EDE.0000000000001011","article-title":"Triclosan and female reproductive health: a preconceptional cohort study","volume":"30","author":"Zhu","year":"2019","journal-title":"Epidemiology"},{"key":"2022072512085861200_ref14","doi-asserted-by":"crossref","first-page":"e019707","DOI":"10.1136\/bmjopen-2017-019707","article-title":"Environmental exposure to triclosan and polycystic ovary syndrome: a cross-sectional study in China","volume":"8","author":"Ye","year":"2018","journal-title":"BMJ Open"},{"key":"2022072512085861200_ref15","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1055\/s-0038-1676489","article-title":"Associations between maternal exposure to bisphenol A or triclosan and gestational hypertension and preeclampsia: the MIREC study","volume":"36","author":"Camara","year":"2019","journal-title":"Am J Perinatol"},{"key":"2022072512085861200_ref16","doi-asserted-by":"crossref","first-page":"18252","DOI":"10.1038\/srep18252","article-title":"Triclosan causes spontaneous abortion accompanied by decline of estrogen sulfotransferase activity in humans and mice","volume":"5","author":"Wang","year":"2015","journal-title":"Sci Rep"},{"key":"2022072512085861200_ref17","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1016\/j.reprotox.2012.09.008","article-title":"Disruption of blastocyst implantation by triclosan in mice: impacts of repeated and acute doses and combination with bisphenol-a","volume":"34","author":"Crawford","year":"2012","journal-title":"Reprod Toxicol"},{"key":"2022072512085861200_ref18","doi-asserted-by":"crossref","first-page":"12993","DOI":"10.1007\/s11356-020-11287-w","article-title":"Triclosan exposure and in vitro fertilization treatment outcomes in women undergoing in vitro fertilization","volume":"28","author":"Radwan","year":"2021","journal-title":"Environ Sci Pollut Res Int"},{"key":"2022072512085861200_ref19","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1530\/REP-16-0501","article-title":"Urinary triclosan concentrations and early outcomes of in vitro fertilization-embryo transfer","volume":"153","author":"Hua","year":"2017","journal-title":"Reproduction"},{"key":"2022072512085861200_ref20","doi-asserted-by":"crossref","first-page":"184845","DOI":"10.1155\/2015\/184845","article-title":"Preimplantation exposure to bisphenol A and triclosan may lead to implantation failure in humans","volume":"2015","author":"Yuan","year":"2015","journal-title":"Biomed Res Int"},{"key":"2022072512085861200_ref21","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1053\/j.gastro.2011.10.025","article-title":"Inflammation and disruption of the mucosal architecture in claudin-7-deficient mice","volume":"142","author":"Ding","year":"2012","journal-title":"Gastroenterology"},{"key":"2022072512085861200_ref22","doi-asserted-by":"crossref","first-page":"6958","DOI":"10.1038\/onc.2008.346","article-title":"Transcriptional regulation of cell polarity in EMT and cancer","volume":"27","author":"Moreno-Bueno","year":"2008","journal-title":"Oncogene"},{"key":"2022072512085861200_ref23","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.tem.2019.11.008","article-title":"Uterine luminal epithelium as the transient gateway for embryo implantation","volume":"31","author":"Ye","year":"2020","journal-title":"Trends Endocrinol Metab"},{"key":"2022072512085861200_ref24","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.reprotox.2017.02.009","article-title":"Perinatal administration of bisphenol A alters the expression of tight junction proteins in the uterus and reduces the implantation rate","volume":"69","author":"Mart\u00ednez-Pe\u00f1a","year":"2017","journal-title":"Reprod Toxicol"},{"key":"2022072512085861200_ref25","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.jhazmat.2012.09.038","article-title":"Effects of DEHP on endometrial receptivity and embryo implantation in pregnant mice","volume":"241\u2013242","author":"Li","year":"2012","journal-title":"J Hazard Mater"},{"key":"2022072512085861200_ref26","doi-asserted-by":"crossref","first-page":"745","DOI":"10.18502\/ijrm.v16i12.3680","article-title":"Effects of benzo(a)pyrene on the endometrial receptivity and embryo implantation in mice: an experimental study","volume":"16","author":"Mardanshahi","year":"2018","journal-title":"Int J Reprod Biomed"},{"key":"2022072512085861200_ref27","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1023\/A:1015455709833","article-title":"\u201cPinopodes\u201d and implantation","volume":"3","author":"Lopata","year":"2002","journal-title":"Rev Endocr Metab Disord"},{"key":"2022072512085861200_ref28","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1038\/nrm.2016.80","article-title":"Tight junctions: from simple barriers to multifunctional molecular gates","volume":"17","author":"Zihni","year":"2016","journal-title":"Nat Rev Mol Cell Biol"},{"key":"2022072512085861200_ref29","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1159\/000147282","article-title":"Tight junctions of human uterine epithelial cells change during the menstrual cycle: a morphometric study","volume":"144","author":"Murphy","year":"1992","journal-title":"Acta Anat"},{"key":"2022072512085861200_ref30","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1038\/sj.cr.7290227","article-title":"Uterine receptivity and the plasma membrane transformation","volume":"14","author":"Murphy","year":"2004","journal-title":"Cell Res"},{"key":"2022072512085861200_ref31","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1007\/s00418-012-1052-y","article-title":"Claudin 7 is reduced in uterine epithelial cells during early pregnancy in the rat","volume":"139","author":"Poon","year":"2013","journal-title":"Histochem Cell Biol"},{"key":"2022072512085861200_ref32","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/j.lfs.2016.01.027","article-title":"Assessment of estradiol-induced gene regulation and proliferation in an immortalized mouse immature Sertoli cell line","volume":"148","author":"Kumar","year":"2016","journal-title":"Life Sci"},{"key":"2022072512085861200_ref33","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.freeradbiomed.2016.01.010","article-title":"Activation of classical estrogen receptor subtypes reduces tight junction disruption of brain endothelial cells under ischemia\/reperfusion injury","volume":"92","author":"Shin","year":"2016","journal-title":"Free Radic Biol Med"}],"container-title":["Biology of Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/biolreprod\/advance-article-pdf\/doi\/10.1093\/biolre\/ioac092\/43913467\/ioac092.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/biolreprod\/article-pdf\/107\/1\/349\/45056005\/ioac092.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/biolreprod\/article-pdf\/107\/1\/349\/45056005\/ioac092.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T12:51:22Z","timestamp":1700571082000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/biolreprod\/article\/107\/1\/349\/6584000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,11]]},"references-count":33,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2022,5,11]]},"published-print":{"date-parts":[[2022,7,25]]}},"URL":"https:\/\/doi.org\/10.1093\/biolre\/ioac092","relation":{},"ISSN":["0006-3363","1529-7268"],"issn-type":[{"value":"0006-3363","type":"print"},{"value":"1529-7268","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2022,7]]},"published":{"date-parts":[[2022,5,11]]}}}