{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T21:50:21Z","timestamp":1773784221160,"version":"3.50.1"},"reference-count":52,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2022,5,11]],"date-time":"2022-05-11T00:00:00Z","timestamp":1652227200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["P50HD068157"],"award-info":[{"award-number":["P50HD068157"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["R03HD064628"],"award-info":[{"award-number":["R03HD064628"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32170858"],"award-info":[{"award-number":["32170858"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31970791"],"award-info":[{"award-number":["31970791"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2018YFC1003500"],"award-info":[{"award-number":["2018YFC1003500"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,7,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Although hundreds of knockout mice show infertility as a major phenotype, the causative genic mutations of male infertility in humans remain rather limited. Here, we report the identification of a missense mutation (D136G) in the X-linked TAF7L gene as a potential cause of oligozoospermia in men. The human aspartate (D136) is evolutionally conserved across species, and its change to glycine (G) is predicted to be detrimental. Genetic complementation experiments in budding yeast demonstrate that the conserved aspartate or its analogous asparagine (N) residue in yeast TAF7 is essential for cell viability and thus its mutation to G is lethal. Although the corresponding D144G substitution in the mouse Taf7l gene does not affect male fertility, RNA-seq analyses reveal alterations in transcriptomic profiles in the Taf7l (D144G) mutant testes. These results support TAF7L mutation as a risk factor for oligozoospermia in humans.<\/jats:p>","DOI":"10.1093\/biolre\/ioac093","type":"journal-article","created":{"date-parts":[[2022,5,12]],"date-time":"2022-05-12T14:01:36Z","timestamp":1652364096000},"page":"157-167","source":"Crossref","is-referenced-by-count":6,"title":["Genetic characterization of a missense mutation in the X-linked <i>TAF7L<\/i> gene identified in an oligozoospermic man"],"prefix":"10.1093","volume":"107","author":[{"given":"Li","family":"Ling","sequence":"first","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine , Nanjing Medical University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fangfang","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine , Nanjing Medical University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pinglan","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine , Nanjing Medical University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert D","family":"Oates","sequence":"additional","affiliation":[{"name":"Department of Urology , Boston University Medical Center, Boston, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sherman","family":"Silber","sequence":"additional","affiliation":[{"name":"Infertility Center of St. Louis , St. Luke's Hospital, St. Louis, MO, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cornelia","family":"Kurischko","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences , University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francis C","family":"Luca","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences , University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N Adrian","family":"Leu","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences , University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinwen","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine , Nanjing Medical University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiuling","family":"Yue","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine , Nanjing Medical University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helen","family":"Skaletsky","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute , Whitehead Institute, and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura G","family":"Brown","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute , Whitehead Institute, and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steve G","family":"Rozen","sequence":"additional","affiliation":[{"name":"Centre for Computational Biology , Duke-NUS Graduate Medical School, Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David C","family":"Page","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute , Whitehead Institute, and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P Jeremy","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences , University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ke","family":"Zheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine , Nanjing Medical University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,5,11]]},"reference":[{"issue":"Suppl","key":"2022072512084633100_ref1","first-page":"s41","article-title":"Genetic dissection of mammalian fertility pathways","volume":"4","author":"Matzuk","year":"2002","journal-title":"Nat Cell Biol"},{"key":"2022072512084633100_ref2","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1038\/s41585-018-0003-3","article-title":"Genetics of male infertility","volume":"15","author":"Krausz","year":"2018","journal-title":"Nat Rev Urol"},{"key":"2022072512084633100_ref3","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1093\/humupd\/dmaa034","article-title":"Molecular genetics of infertility: loss-of-function mutations in humans and corresponding knockout\/mutated mice","volume":"27","author":"Jiao","year":"2021","journal-title":"Hum Reprod Update"},{"key":"2022072512084633100_ref4","doi-asserted-by":"crossref","first-page":"1967","DOI":"10.1038\/s41436-020-0916-0","article-title":"A genomics approach to male infertility","volume":"22","author":"Alhathal","year":"2020","journal-title":"Genet Med"},{"key":"2022072512084633100_ref5","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1093\/humrep\/13.suppl_1.33","article-title":"Epidemiology and aetiology of male infertility","volume":"13","author":"Irvine","year":"1998","journal-title":"Hum Reprod"},{"key":"2022072512084633100_ref6","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1111\/cge.12900","article-title":"Single gene defects leading to sperm quantitative anomalies","volume":"91","author":"Mitchell","year":"2017","journal-title":"Clin Genet"},{"key":"2022072512084633100_ref7","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1007\/s00439-019-02101-w","article-title":"The X chromosome and male infertility","volume":"140","author":"Vockel","year":"2021","journal-title":"Hum Genet"},{"key":"2022072512084633100_ref8","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1038\/nm.f.1895","article-title":"The biology of infertility: research advances and clinical challenges","volume":"14","author":"Matzuk","year":"2008","journal-title":"Nat Med"},{"key":"2022072512084633100_ref9","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/humupd\/dmab030","article-title":"A systematic review of the validated monogenic causes of human male infertility: 2020 update and a discussion of emerging gene-disease relationships","volume":"28","author":"Houston","year":"2021","journal-title":"Hum Reprod Update"},{"key":"2022072512084633100_ref10","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1007\/s00439-020-02159-x","article-title":"A framework for high-resolution phenotyping of candidate male infertility mutants: from human to mouse","volume":"140","author":"Houston","year":"2021","journal-title":"Hum Genet"},{"key":"2022072512084633100_ref11","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1038\/86927","article-title":"An abundance of X-linked genes expressed in spermatogonia","volume":"27","author":"Wang","year":"2001","journal-title":"Nat Genet"},{"key":"2022072512084633100_ref12","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1126\/science.7824954","article-title":"Cloning of an intrinsic human TFIID subunit that interacts with multiple transcriptional activators","volume":"267","author":"Chiang","year":"1995","journal-title":"Science"},{"key":"2022072512084633100_ref13","doi-asserted-by":"crossref","first-page":"1847","DOI":"10.1242\/jcs.00391","article-title":"The intracellular localisation of TAF7L, a paralogue of transcription factor TFIID subunit TAF7, is developmentally regulated during male germ-cell differentiation","volume":"116","author":"Pointud","year":"2003","journal-title":"J Cell Sci"},{"key":"2022072512084633100_ref14","doi-asserted-by":"crossref","first-page":"2582","DOI":"10.1128\/MCB.01722-06","article-title":"Abnormal sperm in mice lacking the Taf7l gene","volume":"27","author":"Cheng","year":"2007","journal-title":"Mol Cell Biol"},{"key":"2022072512084633100_ref15","doi-asserted-by":"crossref","first-page":"16886","DOI":"10.1073\/pnas.1317034110","article-title":"Taf7l cooperates with Trf2 to regulate spermiogenesis","volume":"110","author":"Zhou","year":"2013","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2022072512084633100_ref16","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1101\/gad.1613608","article-title":"Meiotic failure in male mice lacking an X-linked factor","volume":"22","author":"Yang","year":"2008","journal-title":"Genes Dev"},{"key":"2022072512084633100_ref17","doi-asserted-by":"crossref","first-page":"79","DOI":"10.2164\/jandrol.109.008193","article-title":"Regulation of male fertility by X-linked genes","volume":"31","author":"Zheng","year":"2010","journal-title":"J Androl"},{"key":"2022072512084633100_ref18","doi-asserted-by":"crossref","first-page":"e00170","DOI":"10.7554\/eLife.00170","article-title":"Dual functions of TAF7L in adipocyte differentiation","volume":"2","author":"Zhou","year":"2013","journal-title":"Elife"},{"key":"2022072512084633100_ref19","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1111\/j.1439-0272.2007.00789.x","article-title":"Mutation analysis of the X-chromosome linked, testis-specific TAF7L gene in spermatogenic failure","volume":"39","author":"Akinloye","year":"2007","journal-title":"Andrologia"},{"key":"2022072512084633100_ref20","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1093\/molehr\/gal020","article-title":"The role of the testis-specific gene hTAF7L in the aetiology of male infertility","volume":"12","author":"Stouffs","year":"2006","journal-title":"Mol Hum Reprod"},{"key":"2022072512084633100_ref21","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1038\/ng0895-383","article-title":"Diverse spermatogenic defects in humans caused by Y chromosome deletions encompassing a novel RNA-binding protein gene","volume":"10","author":"Reijo","year":"1995","journal-title":"Nat Genet"},{"key":"2022072512084633100_ref22","doi-asserted-by":"crossref","first-page":"1290","DOI":"10.1016\/S0140-6736(96)90938-1","article-title":"Severe oligozoospermia resulting from deletions of azoospermia factor gene on Y chromosome","volume":"347","author":"Reijo","year":"1996","journal-title":"Lancet"},{"key":"2022072512084633100_ref23","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1093\/hmg\/5.7.933","article-title":"Human Y chromosome azoospermia factors (AZF) mapped to different subregions in Yq11","volume":"5","author":"Vogt","year":"1996","journal-title":"Hum Mol Genet"},{"key":"2022072512084633100_ref24","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1038\/ng757","article-title":"The AZFc region of the Y chromosome features massive palindromes and uniform recurrent deletions in infertile men","volume":"29","author":"Kuroda-Kawaguchi","year":"2001","journal-title":"Nat Genet"},{"key":"2022072512084633100_ref25","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1086\/342928","article-title":"Recombination between palindromes P5 and P1 on the human Y chromosome causes massive deletions and spermatogenic failure","volume":"71","author":"Repping","year":"2002","journal-title":"Am J Hum Genet"},{"key":"2022072512084633100_ref26","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1038\/s41386-018-0153-2","article-title":"Sex differences in psychiatric disorders: what we can learn from sex chromosome aneuploidies","volume":"44","author":"Green","year":"2019","journal-title":"Neuropsychopharmacology"},{"key":"2022072512084633100_ref27","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1101\/gr.8.12.1229","article-title":"A DNA polymorphism discovery resource for research on human genetic variation","volume":"8","author":"Collins","year":"1998","journal-title":"Genome Res"},{"key":"2022072512084633100_ref28","doi-asserted-by":"crossref","first-page":"1198","DOI":"10.15252\/emmm.201404967","article-title":"TEX11 is mutated in infertile men with azoospermia and regulates genome-wide recombination rates in mouse","volume":"7","author":"Yang","year":"2015","journal-title":"EMBO Mol Med"},{"key":"2022072512084633100_ref29","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1038\/nmeth0810-575","article-title":"MutationTaster evaluates disease-causing potential of sequence alterations","volume":"7","author":"Schwarz","year":"2010","journal-title":"Nat Methods"},{"key":"2022072512084633100_ref30","first-page":"Unit7.20","article-title":"Predicting functional effect of human missense mutations using PolyPhen-2","volume":"Chapter 7","author":"Adzhubei","year":"2013","journal-title":"Curr Protoc Hum Genet"},{"key":"2022072512084633100_ref31","doi-asserted-by":"crossref","first-page":"2745","DOI":"10.1093\/bioinformatics\/btv195","article-title":"PROVEAN web server: a tool to predict the functional effect of amino acid substitutions and indels","volume":"31","author":"Choi","year":"2015","journal-title":"Bioinformatics"},{"key":"2022072512084633100_ref32","doi-asserted-by":"crossref","first-page":"4882","DOI":"10.1007\/s00415-021-10797-y","article-title":"Shared Individual Formulation Therapy (SIFT): an open-label trial of a new therapy accommodating patient heterogeneity in functional neurological disorder","volume":"268","author":"Gutkin","year":"2021","journal-title":"J Neurol"},{"key":"2022072512084633100_ref33","doi-asserted-by":"crossref","first-page":"2341","DOI":"10.1093\/hmg\/11.19.2341","article-title":"Functional substitution for TAF(II)250 by a retroposed homolog that is expressed in human spermatogenesis","volume":"11","author":"Wang","year":"2002","journal-title":"Hum Mol Genet"},{"key":"2022072512084633100_ref34","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1126\/science.285.5429.901","article-title":"Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis","volume":"285","author":"Winzeler","year":"1999","journal-title":"Science"},{"key":"2022072512084633100_ref35","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1038\/nature00935","article-title":"Functional profiling of the Saccharomyces cerevisiae genome","volume":"418","author":"Giaever","year":"2002","journal-title":"Nature"},{"key":"2022072512084633100_ref36","first-page":"1","article-title":"Guide to yeast genetics and molecular biology","volume":"194","author":"Guthrie","year":"1991","journal-title":"Methods Enzymol"},{"key":"2022072512084633100_ref37","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1101\/SQB.1997.062.01.021","article-title":"Analysis of Fgf8 gene function in vertebrate development","volume":"62","author":"Lewandoski","year":"1997","journal-title":"Cold Spring Harb Symp Quant Biol"},{"key":"2022072512084633100_ref38","first-page":"50648","article-title":"Separation of spermatogenic cell types using STA-PUT velocity sedimentation","volume":"80","author":"Bryant","year":"2013","journal-title":"J Vis Exp"},{"key":"2022072512084633100_ref39","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"fastp: an ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"2022072512084633100_ref40","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.3317","volume":"12","author":"Kim","year":"2015","journal-title":"Nat Methods"},{"key":"2022072512084633100_ref41","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/nbt.3122","volume":"33","author":"Pertea","year":"2015","journal-title":"Nat Biotechnol"},{"key":"2022072512084633100_ref42","doi-asserted-by":"crossref","first-page":"W12","DOI":"10.1093\/nar\/gkx428","article-title":"CPC2: a fast and accurate coding potential calculator based on sequence intrinsic features","volume":"45","author":"Kang","year":"2017","journal-title":"Nucleic Acids Res"},{"key":"2022072512084633100_ref43","doi-asserted-by":"crossref","first-page":"e166","DOI":"10.1093\/nar\/gkt646","article-title":"Utilizing sequence intrinsic composition to classify protein-coding and long non-coding transcripts","volume":"41","author":"Sun","year":"2013","journal-title":"Nucleic Acids Res"},{"key":"2022072512084633100_ref44","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1038\/nature11928","article-title":"Circular RNAs are a large class of animal RNAs with regulatory potency","volume":"495","author":"Memczak","year":"2013","journal-title":"Nature"},{"key":"2022072512084633100_ref45","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol"},{"key":"2022072512084633100_ref46","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"edgeR: a Bioconductor package for differential expression analysis of digital gene expression data","volume":"26","author":"Robinson","year":"2010","journal-title":"Bioinformatics"},{"key":"2022072512084633100_ref47","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/0378-1119(94)90583-5","article-title":"The CCG1\/TAFII250 gene is mutated in thermosensitive G1 mutants of the BHK21 cell line derived from golden hamster","volume":"141","author":"Hayashida","year":"1994","journal-title":"Gene"},{"key":"2022072512084633100_ref48","doi-asserted-by":"crossref","first-page":"1984","DOI":"10.1128\/MCB.06305-11","article-title":"The general transcription factor TAF7 is essential for embryonic development but not essential for the survival or differentiation of mature T cells","volume":"32","author":"Gegonne","year":"2012","journal-title":"Mol Cell Biol"},{"key":"2022072512084633100_ref49","doi-asserted-by":"crossref","first-page":"eaaz6830","DOI":"10.1126\/science.aaz6830","article-title":"Mammalian in vitro gametogenesis","volume":"374","author":"Saitou","year":"2021","journal-title":"Science"},{"key":"2022072512084633100_ref50","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1186\/s13059-020-01994-5","article-title":"Reverse-genetics studies of lncRNAs-what we have learnt and paths forward","volume":"21","author":"Gao","year":"2020","journal-title":"Genome Biol"},{"key":"2022072512084633100_ref51","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1093\/jmcb\/mjy003","article-title":"Mouse knockout models reveal largely dispensable but context-dependent functions of lncRNAs during development","volume":"10","author":"Han","year":"2018","journal-title":"J Mol Cell Biol"},{"key":"2022072512084633100_ref52","doi-asserted-by":"crossref","first-page":"eabb7426","DOI":"10.1126\/sciadv.abb7426","article-title":"Circular RNAs from BOULE play conserved roles in protection against stress-induced fertility decline","volume":"6","author":"Gao","year":"2020","journal-title":"Sci Adv"}],"container-title":["Biology of Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/biolreprod\/advance-article-pdf\/doi\/10.1093\/biolre\/ioac093\/44055478\/ioac093.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/biolreprod\/article-pdf\/107\/1\/157\/45056160\/ioac093.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/biolreprod\/article-pdf\/107\/1\/157\/45056160\/ioac093.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,25]],"date-time":"2022-07-25T12:10:53Z","timestamp":1658751053000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/biolreprod\/article\/107\/1\/157\/6583998"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,11]]},"references-count":52,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2022,5,11]]},"published-print":{"date-parts":[[2022,7,25]]}},"URL":"https:\/\/doi.org\/10.1093\/biolre\/ioac093","relation":{},"ISSN":["0006-3363","1529-7268"],"issn-type":[{"value":"0006-3363","type":"print"},{"value":"1529-7268","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2022,7]]},"published":{"date-parts":[[2022,5,11]]}}}