{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T21:33:42Z","timestamp":1792100022957,"version":"4.4.0"},"reference-count":59,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2022,8,17]],"date-time":"2022-08-17T00:00:00Z","timestamp":1660694400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"National Science Centre, Krakow, Poland","award":["2016\/21\/B\/NZ9\/03616"],"award-info":[{"award-number":["2016\/21\/B\/NZ9\/03616"]}]},{"name":"Institute of Animal Reproduction and Food Research"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,12,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>A molecular interaction between maternal endometrium and implanting conceptus can lead to activation of a variety of transcription factors that regulate expression of several genes necessary for the process of embryo implantation. While, signal transducer and activator of transcription 3 (STAT3) is responsible for decidualization and epithelial remodeling in humans and mice, its role in porcine endometrium has not been explored before. In the present study, we observed a pregnancy dependent increase in gene and protein expression of STAT3. Phosphorylated STAT3 was predominantly present in the endometrium of pregnant animals in luminal and glandular epithelium and in the endothelium of blood vessels with a weak staining in stromal cells. Interleukins, IL-1\u03b2 and IL-6, and epidermal growth factor (EGF)-induced STAT3 expression and phosphorylation in endometrial explants collected on Day 13 of the estrous cycle. Biological significance of STAT3 was evaluated by blocking its phosphorylation with STAT3-specific inhibitor, Stattic. Using porcine extracellular matrix (ECM) and adhesion molecule array, EGF was shown to induce changes in gene expression of ECM components: MMP1, MMP3, MMP12, LAMA1, SELL, and ICAM1, which was abrogated in the presence of Stattic. Transcriptional activity of STAT3 was observed in promoter regions of MMP3 and MMP12. Additionally, IL-6-induced STAT3 phosphorylation upregulated VEGF and VCAM1 abundances in endometrial-endothelial cells (EEC). Moreover, IL-6 resulted in an increase in EEC proliferation and capillary formation which was reversed in the presence of Stattic. Results of present study reveal a role for STAT3 phosphorylation in regulating extracellular matrix remodeling and angiogenesis in porcine endometrium to facilitate embryo implantation.<\/jats:p>","DOI":"10.1093\/biolre\/ioac163","type":"journal-article","created":{"date-parts":[[2022,8,17]],"date-time":"2022-08-17T18:57:01Z","timestamp":1660762621000},"page":"1503-1516","source":"Crossref","is-referenced-by-count":14,"title":["STAT3 in porcine endometrium during early pregnancy induces changes in extracellular matrix components and promotes angiogenesis"],"prefix":"10.1093","volume":"107","author":[{"given":"Beenu Moza","family":"Jalali","sequence":"first","affiliation":[{"name":"Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences , Olsztyn , Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pawel","family":"Likszo","sequence":"additional","affiliation":[{"name":"Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences , Olsztyn , Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karolina","family":"Lukasik","sequence":"additional","affiliation":[{"name":"Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences , Olsztyn , Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,8,17]]},"reference":[{"key":"2022121508062733900_ref1","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/j.anireprosci.2004.04.014","article-title":"Conceptus signals for establishment and maintenance of pregnancy","volume":"82-83","author":"Spencer","year":"2004","journal-title":"Anim Reprod Sci"},{"key":"2022121508062733900_ref2","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1186\/2049-1891-5-51","article-title":"Cytokines from 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