{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:14:49Z","timestamp":1787008489984,"version":"build-2736575974"},"reference-count":193,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2022,10,3]],"date-time":"2022-10-03T00:00:00Z","timestamp":1664755200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100008708","name":"Australian Wool Innovation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008708","id-type":"DOI","asserted-by":"publisher"}]},{"name":"McCaughey Memorial Institute"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,2,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Cryopreserved ram spermatozoa are limited in their capacity to traverse the ovine cervix and achieve fertilization. This altered interaction may be related to modified molecular communication between frozen-thawed ram spermatozoa, seminal plasma, and the female tract. As such, this review aims to identify the biological processes which underpin sperm maturation and transport throughout the female reproductive tract to elucidate factors which may alter this natural process in cryopreserved ram spermatozoa. We also assess critical barriers to ram spermatozoa specific to the ovine cervix and the role of seminal plasma in mitigating these barriers. Transcriptomics is explored as a new approach to understand the sperm\u2013cervix interaction. Recent studies have demonstrated that both spermatozoa and seminal plasma contain a complex profile of coding and non-coding RNAs. These molecular species have clear links with functional fertility, and mounting evidence suggests they may be altered by cryopreservation. Emerging in vitro cell culture models are also investigated as a \u201cnext step\u201d in studying this interaction, utilizing transcriptomics to identify subtle changes in female tract gene expression in response to spermatozoa. The application of such models is proposed as an exciting opportunity to investigate the unique challenges faced by cryopreserved spermatozoa traversing the ovine cervix prior to fertilization.<\/jats:p>","DOI":"10.1093\/biolre\/ioac188","type":"journal-article","created":{"date-parts":[[2022,10,3]],"date-time":"2022-10-03T13:59:09Z","timestamp":1664805549000},"page":"183-196","source":"Crossref","is-referenced-by-count":9,"title":["Molecular insights to the sperm\u2013cervix interaction and the consequences for cryopreserved sperm"],"prefix":"10.1093","volume":"108","author":[{"given":"Sophie","family":"Warr","sequence":"first","affiliation":[{"name":"The University of Sydney School of Life and Environmental Sciences, Faculty of Science, , Sydney, NSW , Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taylor","family":"Pini","sequence":"additional","affiliation":[{"name":"The University of Queensland School of Veterinary Science, , Gatton, QLD , Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon P","family":"de Graaf","sequence":"additional","affiliation":[{"name":"The University of Sydney School of Life and Environmental Sciences, Faculty of Science, , Sydney, NSW , Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica P","family":"Rickard","sequence":"additional","affiliation":[{"name":"The University of Sydney School of Life and Environmental Sciences, Faculty of Science, , Sydney, NSW , Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,10,3]]},"reference":[{"key":"2023021512493354900_","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/S0378-4320(00)00155-X","article-title":"Storage of ram semen","volume":"62","author":"Salamon","year":"2000","journal-title":"Anim Reprod Sci"},{"key":"2023021512493354900_","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0378-4320(94)01328-J","article-title":"Frozen storage of ram semen II. 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