{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T05:18:29Z","timestamp":1777526309745,"version":"3.51.4"},"reference-count":56,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T00:00:00Z","timestamp":1667347200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"KNOW Consortium \u201cHealthy Animal\u2014Safe Food,\u201d MS&HE Decision","award":["05-1\/KNOW2\/2015"],"award-info":[{"award-number":["05-1\/KNOW2\/2015"]}]},{"DOI":"10.13039\/501100004383","name":"Institute of Animal Reproduction and Food Research of Polish Academy of Sciences","doi-asserted-by":"publisher","award":["2\/FBW\/2022"],"award-info":[{"award-number":["2\/FBW\/2022"]}],"id":[{"id":"10.13039\/501100004383","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004442","name":"National Science Centre, Poland","doi-asserted-by":"publisher","award":["2017\/27\/B\/NZ9\/03014"],"award-info":[{"award-number":["2017\/27\/B\/NZ9\/03014"]}],"id":[{"id":"10.13039\/501100004442","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>During early pregnancy, porcine conceptuses (the embryos with associated membranes) secrete estradiol-17\u03b2 (E2)\u2014their major signal for maternal recognition of pregnancy\u2014and prostaglandin E2 (PGE2). Both hormones induce prominent changes of the endometrial transcriptome in vivo. Studies on endometrial pathologies have shown that E2 affects gene expression by epigenetic mechanisms related to DNA methylation. Herein, we determined the effects of E2 and PGE2 alone, and a combined E2\u2009+\u2009PGE2 treatment administered into the uterine lumen in vivo on the expression and activity of DNA-methyltransferases (DNMTs) and on CpG methylation patterns of selected genes in porcine endometrium. To compare the effect of treatment with the physiological effect of pregnancy, endometria from day 12 pregnant\/cyclic gilts were included. Both E2 and PGE2 significantly reduced the expression of DNMTs. Likewise, the expressions of DNMT1 and DNMT3A were decreased on day 12 of pregnancy compared to the estrous cycle. DNMT activity increased in endometrial samples following E2 treatment and in gilts on day 12 of pregnancy. Treatment with E2 alone and\/or simultaneously with PGE2 altered endometrial DNA methylation of CpG sites of ADAMTS20, ADH1C, BGN, PSAT1, and WNT5A. Different CpG methylation patterns of ADAMTS20, BGN, DMBT1, RASSF1, and WNT5A were found in the endometrium on day 12 of pregnancy compared to day 12 of the estrous cycle. Significant correlations were detected between CpG methylation and gene expression for ADAMTS20, ADH1C, BGN, DMBT1, PSAT1, and WNT5A. Our results indicate that CpG methylation induced by embryonic signals may contribute to regulating endometrial gene expression during pregnancy establishment.<\/jats:p>","DOI":"10.1093\/biolre\/ioac193","type":"journal-article","created":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T16:01:37Z","timestamp":1667404897000},"page":"150-168","source":"Crossref","is-referenced-by-count":2,"title":["Novel role for conceptus signals in mRNA expression regulation by DNA methylation in porcine endometrium during early pregnancy"],"prefix":"10.1093","volume":"108","author":[{"given":"Piotr","family":"Kaczynski","sequence":"first","affiliation":[{"name":"Institute of Animal Reproduction and Food Research, Polish Academy of Sciences , Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vera","family":"van der Weijden","sequence":"additional","affiliation":[{"name":"Institute of Agricultural Sciences ETH Zurich, Animal Physiology, , Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ewelina","family":"Goryszewska-Szczurek","sequence":"additional","affiliation":[{"name":"Institute of Animal Reproduction and Food Research, Polish Academy of Sciences , Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"Baryla","sequence":"additional","affiliation":[{"name":"Institute of Animal Reproduction and Food Research, Polish Academy of Sciences , Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanne E","family":"Ulbrich","sequence":"additional","affiliation":[{"name":"Institute of Agricultural Sciences ETH Zurich, Animal Physiology, , Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnieszka","family":"Waclawik","sequence":"additional","affiliation":[{"name":"Institute of Animal Reproduction and Food Research, Polish Academy of Sciences , Olsztyn, 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