{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T22:58:23Z","timestamp":1777157903261,"version":"3.51.4"},"reference-count":59,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2022,10,29]],"date-time":"2022-10-29T00:00:00Z","timestamp":1667001600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,2,13]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Membrane fusion in sperm cells is crucial for acrosomal exocytosis and must be preserved to ensure fertilizing capacity. Evolutionarily conserved protein machinery regulates acrosomal exocytosis. Molecular chaperones play a vital role in spermatogenesis and post-testicular maturation. Cysteine string protein (CSP) is a member of the Hsp40 co-chaperones, and the participation of molecular chaperones in acrosomal exocytosis is poorly understood. In particular, the role of CSP in acrosomal exocytosis has not been reported so far. Using western blot and indirect immunofluorescence, we show that CSP is present in human sperm, is palmitoylated, and predominantly bound to membranes. Moreover, using functional assays and transmission electron microscopy, we report that blocking the function of CSP avoided the assembly of trans-complexes and inhibited exocytosis. In summary, here, we describe the presence of CSP in human sperm and show that this protein has an essential role in membrane fusion during acrosomal exocytosis mediating the trans-SNARE complex assembly between the outer acrosomal and plasma membranes. In general, understanding CSP\u2019s role is critical in identifying new biomarkers and generating new rational-based approaches to treat male infertility.<\/jats:p>","DOI":"10.1093\/biolre\/ioac196","type":"journal-article","created":{"date-parts":[[2022,10,29]],"date-time":"2022-10-29T13:38:10Z","timestamp":1667050690000},"page":"229-240","source":"Crossref","is-referenced-by-count":8,"title":["The molecular chaperone cysteine string protein is required for monomeric SNARE proteins to assemble in<i>trans<\/i>-complexes during human sperm acrosomal exocytosis"],"prefix":"10.1093","volume":"108","author":[{"given":"Karina","family":"Flores-Montero","sequence":"first","affiliation":[{"name":"Universidad Nacional de Cuyo Instituto de Histolog\u00eda y Embriolog\u00eda de Mendoza \u2013 Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, , Mendoza, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Victoria","family":"Berberi\u00e1n","sequence":"additional","affiliation":[{"name":"Universidad Nacional de Cuyo Instituto de Histolog\u00eda y Embriolog\u00eda de Mendoza \u2013 Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, , Mendoza, Argentina"},{"name":"Universidad Nacional de Cuyo Facultad de Ciencias Exactas y Naturales, , Mendoza, Argentina"},{"name":"Universidad Nacional de Cuyo Instituto de Ciencias B\u00e1sicas \u2013 Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, , Mendoza, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis Segundo","family":"Mayorga","sequence":"additional","affiliation":[{"name":"Universidad Nacional de Cuyo Instituto de Histolog\u00eda y Embriolog\u00eda de Mendoza \u2013 Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, , Mendoza, Argentina"},{"name":"Universidad Nacional de Cuyo Facultad de Ciencias Exactas y Naturales, , Mendoza, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia Nora","family":"Tomes","sequence":"additional","affiliation":[{"name":"Universidad Nacional de Cuyo Instituto de Histolog\u00eda y Embriolog\u00eda de Mendoza \u2013 Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, , Mendoza, Argentina"},{"name":"Universidad Nacional de Cuyo Facultad de Ciencias Exactas y Naturales, , Mendoza, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Celeste","family":"Ruete","sequence":"additional","affiliation":[{"name":"Universidad Nacional de Cuyo Instituto de Histolog\u00eda y Embriolog\u00eda de Mendoza \u2013 Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, , Mendoza, Argentina"},{"name":"Universidad Nacional de Cuyo Facultad de Ciencias M\u00e9dicas, , Mendoza, 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