{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T21:16:51Z","timestamp":1781126211212,"version":"3.54.1"},"reference-count":15,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T00:00:00Z","timestamp":1672876800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R03 HD100652"],"award-info":[{"award-number":["R03 HD100652"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R03 HD097384"],"award-info":[{"award-number":["R03 HD097384"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,3,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Forkhead box protein A2 (FOXA2) is a pioneer transcription factor important for epithelial budding and morphogenesis in different organs. It has been used as a specific marker for uterine glandular epithelial cells (GE). FOXA2 has close interactions with estrogen receptor \u03b1 (ER\u03b1). ER\u03b1 binding to Foxa2 gene in the uterus indicates its regulation of Foxa2. The intimate interactions between ER\u03b1 and FOXA2 and their essential roles in early pregnancy led us to investigate the expression of FOXA2 in the female reproductive tract of pre-implantation epiER\u03b1\u2212\/\u2212 (Esr1fl\/flWnt7aCre\/+) mice, in which ER\u03b1 is conditionally deleted in the epithelium of reproductive tract. In the oviduct, FOXA2 is detected in the ciliated epithelial cells of ampulla but absent in the isthmus of day 3.5 post-coitum (D3.5) Esr1fl\/fl control and epiER\u03b1\u2212\/\u2212 mice. In the uterus, FOXA2 expression in the GE appears to be comparable between Esr1fl\/fl and epiER\u03b1\u2212\/\u2212 mice. However, FOXA2 is upregulated in the D0.5 and D3.5 but not PND25-28 epiER\u03b1\u2212\/\u2212 uterine luminal epithelial cells (LE). In the vagina, FOXA2 expression is low in the basal layer and increases toward the superficial layer of the D3.5 Esr1fl\/fl vaginal epithelium, but FOXA2 is detected in the basal, intermediate, and superficial layers, with the strongest FOXA2 expression in the intermediate layers of the D3.5 epiER\u03b1\u2212\/\u2212 vaginal epithelium. This study demonstrates that loss of ER\u03b1 in LE and vaginal basal layer upregulates FOXA2 expression in these epithelial cells during early pregnancy. The mechanisms for epithelial cell-type specific regulation of FOXA2 by ER\u03b1 remain to be elucidated.<\/jats:p>","DOI":"10.1093\/biolre\/ioac225","type":"journal-article","created":{"date-parts":[[2023,1,8]],"date-time":"2023-01-08T03:34:21Z","timestamp":1673148861000},"page":"359-362","source":"Crossref","is-referenced-by-count":10,"title":["Upregulation of FOXA2 in uterine luminal epithelium and vaginal basal epithelium of <i>epiER\u03b1<\/i>\u2212\/\u2212 (<i>Esr1fl\/flWnt7aCre\/+<\/i>) mice"],"prefix":"10.1093","volume":"108","author":[{"given":"Jonathan Matthew","family":"Hancock","sequence":"first","affiliation":[{"name":"Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia , Athens , USA"},{"name":"Interdisciplinary Toxicology Program, University of Georgia , Athens , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuehuan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia , Athens , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taylor Elijah","family":"Martin","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia , Athens , USA"},{"name":"Interdisciplinary Toxicology Program, University of Georgia , Athens , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian Lee","family":"Andersen","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia , Athens , USA"},{"name":"Interdisciplinary Toxicology Program, University of Georgia , Athens , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqin","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology, College of Veterinary Medicine, University 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