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In general, cholesterol acceptors of albumin and \u03b2-cyclodextrins are used to induce capacitation during in vitro fertilization. Previously, we reported that methyl-\u03b2-cyclodextrin (MBCD), which is composed of seven glucoses, had a higher ability to induce capacitation than bovine serum albumin (BSA) in frozen\u2013thawed mouse sperm. Comparison of albumin and cyclodextrins is helpful for understanding the mechanism of capacitation. In this study, we examined the effects of albumin, MBCD, and a different type of cyclodextrin, dimethyl-\u03b1-cyclodextrin (DMACD), which is composed of six glucoses, on several events of sperm capacitation. We showed that DMACD induced sperm capacitation and promoted fertilization ability. The time required to increase the fertilization rate differed among BSA, MBCD, and DMACD. BSA and MBCD enhanced cholesterol and phospholipid efflux, whereas DMACD enhanced only phospholipid efflux. BSA, MBCD, and DMACD increased sperm membrane fluidity, rearrangement of the lipid raft, and the acrosome reaction. These findings suggest that phospholipid efflux is a novel trigger of capacitation. Increasing the choice of sperm capacitation inducers may be useful for improving in vitro fertilization (IVF) techniques not only in mice, but also in various species in which it has been difficult to produce embryos by IVF.<\/jats:p>","DOI":"10.1093\/biolre\/ioad013","type":"journal-article","created":{"date-parts":[[2023,2,2]],"date-time":"2023-02-02T00:21:14Z","timestamp":1675297274000},"page":"671-681","source":"Crossref","is-referenced-by-count":5,"title":["Dimethyl-\u03b1-cyclodextrin induces capacitation by removing phospholipids from the plasma membrane of mouse sperm"],"prefix":"10.1093","volume":"108","author":[{"given":"Satohiro","family":"Nakao","sequence":"first","affiliation":[{"name":"Kumamoto University Division of Reproductive Engineering, Center for Animal Resources and Development, , Kumamoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kotono","family":"Ito","sequence":"additional","affiliation":[{"name":"Kumamoto University Division of Reproductive Engineering, Center for Animal Resources and Development, , Kumamoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhito","family":"Sakoh","sequence":"additional","affiliation":[{"name":"Kumamoto University Division of Reproductive Engineering, Center for Animal Resources and Development, , Kumamoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Takemoto","sequence":"additional","affiliation":[{"name":"Kumamoto University Division of Reproductive Engineering, Center for Animal Resources and Development, , Kumamoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitomi","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Institute for Life and Medical Sciences, Kyoto University Laboratory of Integrative Biological Science, , Kyoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gen","family":"Kondoh","sequence":"additional","affiliation":[{"name":"Institute for Life and Medical Sciences, Kyoto University Laboratory of Integrative Biological Science, , Kyoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsumi","family":"Irie","sequence":"additional","affiliation":[{"name":"Kumamoto University Department of Pharmaceutical Packaging Technology, Global Center for Natural Resources Science, Faculty of Life Sciences, , Kumamoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naomi","family":"Nakagata","sequence":"additional","affiliation":[{"name":"Kumamoto University Division of Reproductive Biotechnology and Innovation, Center for Animal Resources and Development, , Kumamoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toru","family":"Takeo","sequence":"additional","affiliation":[{"name":"Kumamoto University Division of Reproductive Engineering, Center for Animal Resources and Development, , Kumamoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,2,1]]},"reference":[{"key":"2023041705311061700_","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1038\/168697b0","article-title":"Fertilizing capacity of spermatozoa deposited into the fallopian tubes","volume":"168","author":"Chang","year":"1951","journal-title":"Nature"},{"key":"2023041705311061700_","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1172\/JCI0216392","article-title":"The role of cholesterol efflux in regulating the fertilization potential of mammalian spermatozoa","volume":"110","author":"Travis","year":"2002","journal-title":"J Clin Invest"},{"key":"2023041705311061700_","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1095\/biolreprod32.1.145","article-title":"Albumin-mediated changes in sperm sterol content during capacitation","volume":"32","author":"Go","year":"1985","journal-title":"Biol Reprod"},{"key":"2023041705311061700_","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1002\/mrd.1120200209","article-title":"Identification of sterol acceptors that stimulate cholesterol efflux from human spermatozoa during in vitro capacitation","volume":"20","author":"Langlais","year":"1988","journal-title":"Gamete Res"},{"key":"2023041705311061700_","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1042\/bj3350433","article-title":"Effects of cholesterol depletion by cyclodextrin on the sphingolipid microdomains of the plasma membrane","volume":"335","author":"Ilangumaran","year":"1998","journal-title":"Biochem J"},{"key":"2023041705311061700_","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/0005-2736(79)90260-8","article-title":"Studies on the mechanism of capacitation. 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