{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T03:31:27Z","timestamp":1788492687360,"version":"build-2803163510"},"reference-count":73,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2023,3,24]],"date-time":"2023-03-24T00:00:00Z","timestamp":1679616000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Inner Mongolia Autonomous Region Science and Technology Major Project","award":["2022ZD0008"],"award-info":[{"award-number":["2022ZD0008"]}]},{"name":"Inner Mongolia Autonomous Region Science and Technology Major Project","award":["2021ZD0008"],"award-info":[{"award-number":["2021ZD0008"]}]},{"name":"Inner Mongolia Autonomous Region Open Competition Projects","award":["2022JBGS0025"],"award-info":[{"award-number":["2022JBGS0025"]}]},{"name":"Natural Key R&D Project of China","award":["2020YFA0113200"],"award-info":[{"award-number":["2020YFA0113200"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32272877"],"award-info":[{"award-number":["32272877"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31970814"],"award-info":[{"award-number":["31970814"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,6,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The regulation of mammalian early-embryonic development is a complex, coordinated process that involves widespread transcriptomic and epigenetic remodeling. The main cause of developmental failure in preimplantation embryos after in vitro fertilization is the irreversible arrested-at-cleavage stage. To deepen our understanding of this embryonic block, we profiled a single-cell multi-omics map of copy number variations (CNVs), the transcriptome, the DNA methylome, and the chromatin state of bovine eight-cell embryos with a two-cell fate that either arrested or developed into blastocysts. To do this, we sequenced a biopsied blastomere and tracked the developmental potential of the remaining cells. Aneuploid embryos inferred by CNVs from DNA- and RNA-library data tended to lose their developmental potency. Analysis of distinct genomic regions of DNA methylation and chromatin accessibility revealed that enrichment of gene function and signaling pathways, such as the MAPK signaling pathway, was altered in arrested euploid eight-cell embryos compared with blastocyst-developed euploid eight-cell embryos. Moreover, the RNA expression and chromatin accessibility of embryonic genome activation-associated genes were lower in arrested euploid embryos than in blastocyst-developed embryos. Taken together, our results indicate that the developmental block of eight-cell embryos can be caused by multiple molecular layers, including CNVs, abnormality of DNA methylation and chromatin accessibility, and insufficient expression of embryonic genome activation-associated genes. Our integrated and comprehensive data set provides a valuable resource to further dissect the exact mechanisms underlying the arrest of bovine eight-cell embryos in vitro.<\/jats:p>","DOI":"10.1093\/biolre\/ioad038","type":"journal-article","created":{"date-parts":[[2023,3,24]],"date-time":"2023-03-24T15:46:53Z","timestamp":1679672813000},"page":"871-886","source":"Crossref","is-referenced-by-count":10,"title":["Dissecting the molecular features of bovine-arrested eight-cell embryos using single-cell multi-omics sequencing"],"prefix":"10.1093","volume":"108","author":[{"given":"Jingyao","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, College of Animal Science and Technology, China Agricultural University , Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingji","family":"Lyu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, College of Animal Science and Technology, China Agricultural University , Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, College of Animal Science and Technology, China Agricultural University , Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuoran","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, College of Animal Science and Technology, China Agricultural University , Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruoyu","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, College of Animal Science and Technology, China Agricultural University , Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xunzhe","family":"Yin","sequence":"additional","affiliation":[{"name":"School of Municipal and Environmental Engineering, Shandong Jianzhu University , Jinan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University , Hohhot, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Gao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, College of Animal Science and Technology, China Agricultural University , Beijing, China"},{"name":"Beijing Jingwa Agricultural Science & Technology Innovation Center , Beijing 101202, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,3,24]]},"reference":[{"key":"2023061501033659500_ref1","doi-asserted-by":"crossref","first-page":"2832","DOI":"10.1093\/humrep\/deaa250","article-title":"20 years of the European IVF-monitoring consortium registry: what have we learned? 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