{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T13:33:20Z","timestamp":1782135200207,"version":"3.54.5"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2023,5,8]],"date-time":"2023-05-08T00:00:00Z","timestamp":1683504000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100021171","name":"Guangdong Basic and Applied Basic Research Foundation","doi-asserted-by":"publisher","award":["2021A1515011264"],"award-info":[{"award-number":["2021A1515011264"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Guangdong Basic and Applied Basic Research Foundation","doi-asserted-by":"publisher","award":["2023A1515012011"],"award-info":[{"award-number":["2023A1515012011"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Guangzhou Science and Technology Planning Project","award":["2023A04J0150"],"award-info":[{"award-number":["2023A04J0150"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31600969"],"award-info":[{"award-number":["31600969"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006469","name":"Macao Science and Technology Development Fund","doi-asserted-by":"publisher","award":["001\/2020\/ALC"],"award-info":[{"award-number":["001\/2020\/ALC"]}],"id":[{"id":"10.13039\/501100006469","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,7,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Aerobic exercises could improve the sperm motility of obese individuals. However, the underlying mechanism has not been fully elucidated, especially the possible involvement of the epididymis in which sperm acquire their fertilizing capacity. This study aims to investigate the benefit effect of aerobic exercises on the epididymal luminal milieu of obese rats. Sprague\u2013Dawley male rats were fed on a normal or high-fat diet (HFD) for 10 weeks and then subjected to aerobic exercises for 12\u00a0weeks. We verified that TRPA1 was located in the epididymal epithelium. Notably, aerobic exercises reversed the downregulated TRPA1 in the epididymis of HFD-induced obese rats, thus improving sperm fertilizing capacity and Cl\u2212 concentration in epididymal milieu. Ussing chamber experiments showed that cinnamaldehyd (CIN), agonist of TRPA1, stimulated an increase of the short-circuit current (ISC) in rat cauda epididymal epithelium, which was subsequently abolished by removing the ambient Cl\u2212 and HCO3\u2212. In vivo data revealed that aerobic exercises increased the CIN-stimulated Cl\u2212 secretion rate of epididymal epithelium in obese rats. Pharmacological experiments revealed that blocking cystic fibrosis transmembrane regulator (CFTR) and Ca2+-activated Cl\u2212 channel (CaCC) suppressed the CIN-stimulated anion secretion. Moreover, CIN application in rat cauda epididymal epithelial cells elevated intracellular Ca2+ level, and thus activate CACC. Interfering with the PGHS2-PGE2-EP2\/EP4-cAMP pathway suppressed CFTR-mediated anion secretion. This study demonstrates that TRPA1 activation can stimulate anion secretion via CFTR and CaCC, which potentially forming an appropriate microenvironment essential for sperm maturation, and aerobic exercises can reverse the downregulation of TRPA1 in the epididymal epithelium of obese rats.<\/jats:p>","DOI":"10.1093\/biolre\/ioad050","type":"journal-article","created":{"date-parts":[[2023,5,8]],"date-time":"2023-05-08T13:04:30Z","timestamp":1683551070000},"page":"53-64","source":"Crossref","is-referenced-by-count":2,"title":["Aerobic exercises regulate the epididymal anion homeostasis of high-fat diet-induced obese rats through TRPA1-mediated Cl\u2212 and HCO3\u2212 secretion"],"prefix":"10.1093","volume":"109","author":[{"given":"Dong-Dong","family":"Gao","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Physical Activity and Health Promotion , Scientific Research Center, , Guangzhou, Guangdong , China"},{"name":"Guangzhou Sport University , Scientific 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Promotion , Scientific Research Center, , Guangzhou, Guangdong , China"},{"name":"Guangzhou Sport University , Scientific Research Center, , Guangzhou, Guangdong , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Lin","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Physical Activity and Health Promotion , Scientific Research Center, , Guangzhou, Guangdong , China"},{"name":"Guangzhou Sport University , Scientific Research Center, , Guangzhou, Guangdong , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lian-Meng","family":"Guo","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Physical Activity and Health Promotion , Scientific Research Center, , Guangzhou, Guangdong , China"},{"name":"Guangzhou Sport University , Scientific Research Center, , Guangzhou, Guangdong , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Hao","family":"Liang","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Physical Activity and Health Promotion , Scientific Research Center, , Guangzhou, Guangdong , China"},{"name":"Guangzhou Sport University , Scientific Research Center, , Guangzhou, Guangdong , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Hui","family":"Zhong","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Physical Activity and Health Promotion , Scientific Research Center, , Guangzhou, Guangdong , China"},{"name":"Guangzhou Sport University , Scientific Research Center, , Guangzhou, Guangdong , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing-Wen","family":"Liao","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Physical Activity and Health Promotion , Scientific Research Center, , Guangzhou, Guangdong , China"},{"name":"Guangzhou Sport University , Scientific Research Center, , Guangzhou, Guangdong , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun-Hao","family":"Huang","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Physical Activity and Health Promotion , Scientific Research Center, , Guangzhou, Guangdong , China"},{"name":"Guangzhou Sport University , Scientific Research Center, , Guangzhou, Guangdong , China"},{"name":"Dr Neher\u2019s Biophysics Laboratory for Innovative Drug Discovery , State Key Laboratory of Quality Research in Chinese Medicine, , Macau , China"},{"name":"Macau University of Science and Technology , State Key Laboratory of Quality Research in Chinese Medicine, , Macau , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Hu","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Physical Activity and 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