{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T17:25:30Z","timestamp":1775064330191,"version":"3.50.1"},"reference-count":25,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2023,8,2]],"date-time":"2023-08-02T00:00:00Z","timestamp":1690934400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008695","name":"Japan Foundation for Applied Enzymology","doi-asserted-by":"publisher","award":["2022-12"],"award-info":[{"award-number":["2022-12"]}],"id":[{"id":"10.13039\/100008695","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Futaba Foundation","award":["223074"],"award-info":[{"award-number":["223074"]}]},{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","award":["JPMJPR2143"],"award-info":[{"award-number":["JPMJPR2143"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]},{"name":"The Ministry of Education, Culture, Sports, Science and Technology (MEXT)\/Japan Society for the Promotion of Science (JSPS) KAKENHI","award":["JP21H0503"],"award-info":[{"award-number":["JP21H0503"]}]},{"name":"The Ministry of Education, Culture, Sports, Science and Technology (MEXT)\/Japan Society for the Promotion of Science (JSPS) KAKENHI","award":["JP21K19263"],"award-info":[{"award-number":["JP21K19263"]}]},{"name":"The Ministry of Education, Culture, Sports, Science and Technology (MEXT)\/Japan Society for the Promotion of Science (JSPS) KAKENHI","award":["JP21H00231"],"award-info":[{"award-number":["JP21H00231"]}]},{"name":"The Ministry of Education, Culture, Sports, Science and Technology (MEXT)\/Japan Society for the Promotion of Science (JSPS) KAKENHI","award":["JP21H02487"],"award-info":[{"award-number":["JP21H02487"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,10,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The mammalian epididymis is the organ for functional sperm maturation. In rodents, the initial segment, the most proximal region of the epididymis, plays a critical role in sperm maturation. The luminal epithelial differentiation and the following gene expression of the initial segment are regulated by the lumicrine signaling, a testis-epididymis transluminal secreted signaling. Adhesion G protein-coupled receptor G2 (ADGRG2) is expressed in the efferent duct and the initial segment epididymis. In the preceding study, Adgrg2 ablation decreased the expression of several genes expressed in the initial segment. Such downregulated genes include those known to be regulated by lumicrine signaling, suggesting the involvement of ADGRG2 in lumicrine signaling. The present study examined whether ADGRG2 is associated with the lumicrine signaling regulating epididymal initial segment differentiation and gene expression. Adgrg2-null mice were generated by CRISPR\/CAS9-mediated genome editing. The postnatal differentiation of the Adgrg2-null male epididymal initial segment was histologically comparable with that of control wild-type animals. The RNA-seq of Adgrg2-null mice was performed together with those of efferent duct-ligated and W\/Wv mice in both of which lumicrine signaling is defective. The comparative transcriptome analyses clarified that the expressions of genes expressed in the initial segment and regulated by lumicrine signaling were decreased by Adgrg2 nullification. However, the extent of such downregulations observed in Adgrg2-null epididymis was not so prominent compared with those of lumicrine signaling deficient Nell2\u2212\/\u2212, efferent duct-ligated, or W\/Wv mice. Collectively, these findings indicate that ADGRG2 is dispensable for the lumicrine regulation of epididymal initial segment differentiation.<\/jats:p>","DOI":"10.1093\/biolre\/ioad087","type":"journal-article","created":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T20:48:19Z","timestamp":1690922899000},"page":"474-481","source":"Crossref","is-referenced-by-count":7,"title":["Adhesion G protein-coupled receptor G2 is dispensable for lumicrine signaling regulating epididymal initial segment differentiation and gene expression"],"prefix":"10.1093","volume":"109","author":[{"given":"Daiji","family":"Kiyozumi","sequence":"first","affiliation":[{"name":"Nagoya University Research Institute of Environmental Medicine, , Nagoya, Aichi , Japan"},{"name":"PRESTO, Japan Science and Technology Agency , Kawaguchi, Saitama , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahito","family":"Ikawa","sequence":"additional","affiliation":[{"name":"Osaka University Research 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